FB2024_03 , released June 25, 2024
Allele: Dmel\gwHMS00105
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General Information
Symbol
Dmel\gwHMS00105
Species
D. melanogaster
Name
FlyBase ID
FBal0263110
Feature type
allele
Associated gene
Dmel\gw
Associated Insertion(s)
Carried in Construct
P{TRiP.HMS00105}
Also Known As
HMS00105
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Ni et al., 2011.7.19)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Ni et al., 2011.7.19)
Mutagen
in vitro construct
(Ni et al., 2011.7.19)
Progenitor genotype
Carried in construct
P{TRiP.HMS00105}
Cytology
Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dsRNA-HMS00105
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing gwHMS00105 under the control of Scer\GAL4tim.PE (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency) are arrhythmic for locomotor activity under constant darkness conditions.

      Circadian behaviour under 12 hour light/12 hour dark conditions is altered in flies expressing gwHMS00105 under the control of Scer\GAL4tim.PE (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency) The morning peak of activity is severely blunted, although a robust evening peak is still present. The phase of the evening peak of activity is advanced compared to control flies.

      Flies expressing gwHMS00105 under the control of Scer\GAL4P2.4.Pdf show normal circadian locomotor behaviour under constant darkness conditions.

      (Zhang and Emery, 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of PdfScer\UAS.ML.T:SS-trypsin.T:Hsap\MYC.T:GPI-lynx1 rescues rhythmic locomotor behaviour under constant darkness conditions in 41.0% of flies expressing gwHMS00105 under the control of Scer\GAL4tim.PE (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency).

      Locomotor activity behaviour under 12 hour light/12 hour dark conditions is partially rescued in flies expressing gwHMS00105 under the control of Scer\GAL4tim.PE (in the presence of Dcr-2Scer\UAS.cDa) if they are also mutant for dnc1. The evening peak phase is rescued closer to a wild type value, while the morning peak is not restored. 41.0% of flies expressing gwHMS00105 under the control of Scer\GAL4tim.PE (in the presence of Dcr-2Scer\UAS.cDa) in a dnc1 background are rhythmic for locomotor activity under constant darkness conditions.

      (Zhang and Emery, 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      gwHMS00105 is rescued by gwUAS.RR/Scer\GAL4tim.PE

      (Zhang and Emery, 2013)
      Not rescued by

      gwHMS00105 is not rescued by Scer\GAL4tim.PE/gwAA.UAS.RR

      (Zhang and Emery, 2013)
      Comments

      Co-expression of gwshRNAres.Scer\UAS restores rhythmic locomotor behaviour under constant darkness conditions flies expressing gwHMS00105 under the control of Scer\GAL4tim.PE (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency). The rescued flies often have a long period phenotype. Under 12 hour light/2 hour dark conditions, both the morning peak and the evening peak are normal in phase and amplitude in the rescued flies.

      Co-expression of gwAA.shRNAres.Scer\UAS usually fails to restore rhythmic locomotor behaviour under constant darkness conditions in flies expressing gwHMS00105 under the control of Scer\GAL4tim.PE (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency). The locomotor activity defects seen under 12 hour light/2 hour dark conditions are not rescued.

      (Zhang and Emery, 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      gwHMS00105
      Name Synonyms
      Secondary FlyBase IDs
        References (6)