FB2024_03 , released June 25, 2024
Allele: Dmel\gwAA.UAS.RR
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General Information
Symbol
Dmel\gwAA.UAS.RR
Species
D. melanogaster
Name
FlyBase ID
FBal0286120
Feature type
allele
Associated gene
Dmel\gw
Associated Insertion(s)
Carried in Construct
P{UAS-gw.AA.RR}
Key Links
Transgenic product class
missense_variant
(Zhang and Emery, 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Zhang and Emery, 2013)
Mutagen
in vitro construct
(Zhang and Emery, 2013)
Progenitor genotype
Carried in construct
P{UAS-gw.AA.RR}
Cytology
Description

UASt regulatory sequences drive expression of gw containing a number of synonymous codon mutations such that the binding site for the shRNA encoded by P{TRiP.HMS00105} has been extensively mutated without effecting the amino acid sequence. In addition, the N-terminal glycine-tryptophan (GW) motifs critical for AGO1 binding have been mutated to alanine residues (AA).

(Zhang and Emery, 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
gw
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4tim.PE/gwAA.UAS.RR fails to rescue gwHMS00105

      (Zhang and Emery, 2013)
      Comments

      Co-expression of gwAA.shRNAres.Scer\UAS usually fails to restore rhythmic locomotor behaviour under constant darkness conditions in flies expressing gwHMS00105 under the control of Scer\GAL4tim.PE (in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency). The locomotor activity defects seen under 12 hour light/2 hour dark conditions are not rescued.

      (Zhang and Emery, 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      gwAA.UAS.RR
      gwAA.shRNAres.Scer\UAS
      gwAA.shRNAres.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)