FlyBase Allele Report: Dmel\G9a[UAS.cKa]

General Information

Symbol

Dmel\G9a^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kramer

FlyBase ID

FBal0260883

Feature type

allele

Associated gene

Dmel\G9a

Associated Insertion(s)

Carried in Construct

P{UAS-G9a.K}

Also Known As

UAS-EHMT

Key Links

Transgenic product class

cDNA

(Kramer et al., 2011)

wild_type

(Kramer et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kramer et al., 2011)

wild_type

(Kramer et al., 2011)

Mutagen

in vitro construct

(Kramer et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-G9a.K}

Cytology

Description

UAS drives expression of the LD10243 G9a cDNA.

(Kramer et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

G9a

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage, with G9a^rv+

(Kramer et al., 2011)

lethal, with Scer\GAL4^Act.PU

(Merkling et al., 2015)

lethal, with Scer\GAL4^da.PU

(Merkling et al., 2015)

lethal, with Scer\GAL4^Tub.PU

(Merkling et al., 2015)

Phenotype Manifest In

wing vein, with Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

Detailed Description

Statement

Reference

Overexpression of G9a^Scer\UAS.cKa in the wing, under the control of Scer\GAL4^Bx-MS1096, consistently causes extra veins in defined regions of the wing.

(Kleefstra et al., 2012)

Expression of G9a^Scer\UAS.cKa using Scer\GAL4⁴⁷⁷ in a G9a^rv+ background does not increase dendrite branching of type 4 multidendritic neurons.

Expression of G9a^Scer\UAS.cKa using Scer\GAL4^elav.PLu in a G9a^rv+ background has no significant effect on short-term courtship memory. However, G9a^Scer\UAS.cKa expression using Scer\GAL4^7B in this background significantly reduces short-term courtship memory of adult males.

(Kramer et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096, trr^GD4501 has lethal - all die before end of P-stage phenotype

(Kleefstra et al., 2012)

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096, trr^JF03242 has lethal - all die before end of P-stage phenotype

(Kleefstra et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, enhanceable by sba^EY22988, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, enhanceable by EcR^A.UAS, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, enhanceable by EcR^B1.UAS, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, enhanceable by EcR^B2.UAS, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

NOT Enhanced by

Statement

Reference

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, non-enhanceable by CG5026^GD11509, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, non-enhanceable by CG5026^KK102042, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

Suppressed by

Statement

Reference

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, suppressible by EcR[+]/EcR^Q50st

(Kleefstra et al., 2012)

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, suppressible by EcR^M554fs/EcR[+]

(Kleefstra et al., 2012)

NOT suppressed by

Statement

Reference

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, non-suppressible by CG5026^GD11509, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096 has wing vein phenotype, non-suppressible by CG5026^KK102042, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

Other

Statement

Reference

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096, trr^GD4501 has wing disc phenotype

(Kleefstra et al., 2012)

G9a^UAS.cKa, Scer\GAL4^Bx-MS1096, trr^JF03242 has wing disc phenotype

(Kleefstra et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of sba^EY22988 enhances the wing vein phenotype of G9a^Scer\UAS.cKa expression under the control of Scer\GAL4^Bx-MS1096. There is a consistent disruption of vein patterning and a strong increase in ectopic vein formation.

Co-expression of trr^GD4501 with G9a^Scer\UAS.cKa overexpression (both under the control of Scer\GAL4^Bx-MS1096) results in fully penetrant pupal lethality resulting from necrosis of the entire developing wing tissue.

Co-expression of trr^JF03242 with G9a^Scer\UAS.cKa overexpression (both under the control of Scer\GAL4^Bx-MS1096) results in fully penetrant pupal lethality resulting from necrosis of the entire developing wing tissue.

Expression of CG5026^GD11509 has no effect on the G9a^Scer\UAS.cKa wing vein phenotype.

Expression of CG5026^KK102042 has no effect on the G9a^Scer\UAS.cKa wing vein phenotype.

The G9a^Scer\UAS.cKa wing vein phenotype is almost completely suppressed by a heterozygous EcR^M554fs background.

The G9a^Scer\UAS.cKa wing vein phenotype is almost completely suppressed by a heterozygous EcR^Q50st background.

The G9a^Scer\UAS.cKa wing vein phenotype is almost completely suppressed by co-expression of EcR^GD1428.

Co-expression of EcR^A.Scer\UAS enhances the G9a^Scer\UAS.cKa wing vein phenotype.

Co-expression of EcR^B1.Scer\UAS enhances the G9a^Scer\UAS.cKa wing vein phenotype.

Co-expression of EcR^B2.Scer\UAS enhances the G9a^Scer\UAS.cKa wing vein phenotype.

(Kleefstra et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

G9a^UAS.cKa is rescued by G9a^GD9876/Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

Rescues

G9a^UAS.cKa rescues G9a^DD2

(Merkling et al., 2015)

G9a^UAS.cKa/Scer\GAL4^hs.PB rescues G9a^DD1

(Kramer et al., 2011)

G9a^UAS.cKa/Scer\GAL4^elav.PLu rescues G9a^DD2

(Kramer et al., 2011)

G9a^UAS.cKa/Scer\GAL4^7B rescues G9a^DD2

(Kramer et al., 2011)

Partially rescues

G9a^UAS.cKa/Scer\GAL4⁴⁷⁷ partially rescues G9a^DD1

(Kramer et al., 2011)

G9a^UAS.cKa/Scer\GAL4⁴⁷⁷ partially rescues G9a^DD2

(Kramer et al., 2011)

Fails to rescue

G9a^UAS.cKa/Scer\GAL4^repo fails to rescue G9a^DD2

(Merkling et al., 2015)

Scer\GAL4^Hml.PG/G9a^UAS.cKa fails to rescue G9a^DD2

(Merkling et al., 2015)

G9a^UAS.cKa/Scer\GAL4⁴⁷⁷ fails to rescue G9a^DD1

(Kramer et al., 2011)

G9a^UAS.cKa/Scer\GAL4⁴⁷⁷ fails to rescue G9a^DD2

(Kramer et al., 2011)

Comments

The early lethality of DCV infected G9a^DD2 mutants is rescued to control levels by fat body-specific expression of G9a^Scer\UAS.cKa under the control of Scer\GAL4^c564.

Expression of G9a^Scer\UAS.cKa under the control of either Scer\GAL4^Hml.PG or Scer\GAL4^repo fails to rescue the hypersensitivity of G9a^DD2 mutant flies to DCV infection.

(Merkling et al., 2015)

Expression of G9a^Scer\UAS.cKa using Scer\GAL4⁴⁷⁷ in a G9a^DD1 or G9a^DD2 background rescues dendrite branching towards wild type levels.

Expression of G9a^Scer\UAS.cKa using Scer\GAL4⁴⁷⁷ in a G9a^DD1 or G9a^DD2 background fails to restore normal larval locomotor behaviour.

Expression of G9a^Scer\UAS.cKa using Scer\GAL4^elav.PLu or Scer\GAL4^7B restores the short-term courtship memory defect of G9a^DD2 males.

Induced expression of G9a^Scer\UAS.cKa using Scer\GAL4^hs.PB after eclosion completely restores the short-term courtship memory defects shown by G9a^DD1 mutants.

(Kramer et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

G9a^Scer\UAS.cKa

G9a^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kramer

Secondary FlyBase IDs

References (3)

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