FB2024_03 , released June 25, 2024
Allele: Dmel\mir-315UAS.DsRed(Unk)
Open Close
General Information
Symbol
Dmel\mir-315UAS.DsRed(Unk)
Species
D. melanogaster
Name
FlyBase ID
FBal0258690
Feature type
allele
Associated gene
Dmel\mir-315
Associated Insertion(s)
Carried in Construct
P{UAS-mir-315.DsRed}
Key Links
Transgenic product class
wild_type
(Silver et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Silver et al., 2007)
Mutagen
in vitro construct
(Silver et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-mir-315.DsRed}
Cytology
Description

UAS regulatory sequences drive expression of mir-315 inserted into the 3' UTR of DsRed(Unk).

(Silver et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mir-315
Tagged with
DsRed(Unk)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mis-expression of mir-315Scer\UAS.T:Disc\RFP-DsRed2 in the wing disc under the control of Scer\GAL4ptc-559.1 results in a dramatic double-wing pouch disk. Most animals expressing mir-315Scer\UAS.T:Disc\RFP-DsRed2 under the control of Scer\GAL4ptc-559.1 die during early pupal development, but culturing them at 18[o]C permits some to survive to pharate stages. Dissection of these animals from their pupal cases reveals transformations of scutellar tissue into ectopic wings.

      Expression of mir-315Scer\UAS.T:Disc\RFP-DsRed2 under the control of Scer\GAL4Bx-MS1096 induces lethality.

      (Silver et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of mir-315Scer\UAS.T:Disc\RFP-DsRed2 with NotumEPg31259 under the control of Scer\GAL4ptc-559.1 suppresses the lethality resulting from the expression of NotumEPg31259 alone.

      Activation of NotumEPg31259 by using Scer\GAL4ptc-559.1 significantly suppresses the notum-to_wing transformation and wing pouch duplication induced by mir-315Scer\UAS.T:Disc\RFP-DsRed2.

      When expressed in the presence (or absence) of co-expressed mir-315Scer\UAS.T:Disc\RFP-DsRed2 by using Scer\GAL4ptc-559.1, AxnScer\UAS.T:Avic\GFP induces embryo lethality.

      Co-expression of AxnScer\UAS.T:Avic\GFP with mir-315Scer\UAS.T:Disc\RFP-DsRed2 under the control of Scer\GAL4Bx-MS1096 partially suppresses the lethality induced by mir-315Scer\UAS.T:Disc\RFP-DsRed2 expression.

      (Silver et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      mir-315Scer\UAS.T:Disc\RFP-DsRed2
      mir-315UAS.DsRed(Unk)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)