The concentration of circulating hemocytes in third instar larvae expressing CblL.Δ70Z.Scer\UAS under the control of Scer\GAL4crq.PO is comparable to that in wild-type controls.
Expression of CblL.Δ70Z.Scer\UAS in the follicle cells under the control of Scer\GAL4EQ1 results in a dorsalised eggshell phenotype, characterised by an expanded dorsal midline.
Expression of CblL.Δ70Z.Scer\UAS in the embryo under the control of Scer\GAL4GR1 results in a dorsalised embryo, with severely disrupted ventral denticle belts.
Expression of CblL.Δ70Z.Scer\UAS in the eye under the control of Scer\GAL4GMR.PF results in a rough-eye phenotype.
Expression of CblL.Δ70Z.Scer\UAS in the wing under the control of Scer\GAL4Bx-MS1096 results in an extra-vein phenotype.
CblL.Δ70Z.UAS, Scer\GAL4crq.PO is a non-suppressor of increased cell number | third instar larval stage phenotype of EgfrUAS.cBa, Scer\GAL4crq.PO
CblL.Δ70Z.UAS, Scer\GAL4EQ1 has dorsal appendage phenotype, suppressible by drk[+]/drke0A
CblL.Δ70Z.UAS, Scer\GAL4crq.PO is a non-suppressor of hemocyte | increased number | third instar larval stage phenotype of EgfrUAS.cBa, Scer\GAL4crq.PO
The increased concentration of circulating hemocytes observed in third instar larvae expressing EgfrScer\UAS.cBa under the control of Scer\GAL4crq.PO is not suppressed by co-expression of CblL.Δ70Z.Scer\UAS.
The dorsalised eggshell phenotype observed when CblL.Δ70Z.Scer\UAS is expressed under the control of Scer\GAL4EQ1 is suppressed in a drke0A/+ background.
Scer\GAL4crq.PO/CblL.Δ70Z.UAS fails to rescue CblKG03080
The increased concentration of circulating hemocytes in CblKG03080 homozygous mutant third instar larvae along with the strongly reduced internalization of sSpi-GFP protein (spi encodes the principal ligand of the EGFR pathway) at high sSpi-GFP conditions (treatment with 30 ng/ml sSpi-GFP) seen in primary hemocytes isolated form the mutant larvae cannot be rescued by expression of CblL.Δ70Z.Scer\UAS under the control of Scer\GAL4crq.PO in the CblKG03080 mutants.
