FlyBase Allele Report: Dmel\Cbl[KG03080]

General Information

Symbol

Dmel\Cbl^KG03080

Species

D. melanogaster

Name

FlyBase ID

FBal0134284

Feature type

allele

Associated gene

Dmel\Cbl

Associated Insertion(s)

P{SUPor-P}Cbl^KG03080

Carried in Construct

Also Known As

kg03080

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}Cbl^KG03080

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:8,425,107..8,425,107

(Yu et al., 2012, Dworkin and Gibson, 2006, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

hemocyte | third instar larval stage

(Kim et al., 2017)

wing

(Dworkin and Gibson, 2006)

Detailed Description

Statement

Reference

Cbl^KG03080 homozygous mutant third instar larvae have significantly increased concentration of circulating hemocytes compared to wild-type controls due to an increase in their mitotic activity (higher percentage of pH3-positive hemocytes). Primary hemocytes from these mutant larvae show strongly reduced internalization of sSpi-GFP protein (spi encodes the principal ligand of the EGFR pathway) at high sSpi-GFP conditions (treatment with 30 ng/ml sSpi-GFP), as compared to controls.

(Kim et al., 2017)

Homozygous Cbl^KG03080 flies show poor survival to adulthood.

Less than half of Cbl^EY11427/Cbl^KG03080 mutant males survive to adulthood.

Pupation is delayed by approximately 24 hours in Cbl^EY11427/Cbl^KG03080 larvae compared with wild-type controls.

Cbl^EY11427/Cbl^KG03080 males show increased sensitivity to starvation, exhibiting an approximately 20% decrease in the median survival time upon food deprivation.

Cbl^EY11427/Cbl^KG03080 males display a dramatically shortened lifespan, with an approximately 67% reduction in the median lifespan compared with wild-type controls.

(Yu et al., 2012)

Heterozygotes show a direct effect on the shape of the wing.

(Dworkin and Gibson, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Cbl^KG03080/Cbl[+] is a non-enhancer of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

NOT Suppressor of

Statement

Reference

Cbl^KG03080/Cbl[+] is a non-suppressor of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Cbl^KG03080/Cbl[+] is a non-enhancer of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

NOT Suppressor of

Statement

Reference

Cbl^KG03080/Cbl[+] is a non-suppressor of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cbl^KG03080 is rescued by Scer\GAL4^crq.PO/Cbl^L.UAS

(Kim et al., 2017)

Not rescued by

Cbl^KG03080 is not rescued by Scer\GAL4^crq.PO/Cbl^L.Δ70Z.UAS

(Kim et al., 2017)

Comments

The increased concentration of circulating hemocytes in Cbl^KG03080 homozygous mutant third instar larvae along with the strongly reduced internalization of sSpi-GFP protein (spi encodes the principal ligand of the EGFR pathway) at high sSpi-GFP conditions (treatment with 30 ng/ml sSpi-GFP) seen in primary hemocytes isolated form the mutant larvae is fully rescued by expression of Cbl^L.Scer\UAS but not Cbl^{L.Δ70Z.Scer\UAS} under the control of Scer\GAL4^crq.PO in the Cbl^KG03080 mutants.

(Kim et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

111572

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{SUPor-P}Cbl^KG03080 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Cbl^KG03080

(Oishi et al., 2006)

D-cbl^KG03080

(Kim et al., 2017)

KG03080

(Dworkin et al., 2011)

cbl^kg03080

(Dworkin and Gibson., 2006)

dCbl^KG

(Yu et al., 2012)

kg03080

(Dworkin and Gibson, 2006)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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