FB2024_03 , released June 25, 2024
Allele: Dmel\Bap55LL05955
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General Information
Symbol
Dmel\Bap55LL05955
Species
D. melanogaster
Name
FlyBase ID
FBal0258201
Feature type
allele
Associated gene
Dmel\Bap55
Associated Insertion(s)
PBac{SAstopDsRed}Bap55LL05955
Carried in Construct
Also Known As
LL05955
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
piggyBac activity
(Tea and Luo, 2011)
Progenitor genotype
Associated Insertion(s)
PBac{SAstopDsRed}Bap55LL05955
Cytology
Description

Insertion of PBac{SAstopDsRed} into the coding sequence (2nd exon) of Bap55.

(Tea and Luo, 2011)
Allele components
Component
Use(s)
Inserted element
PBac{SAstopDsRed}
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In Bap55LL05955 mutants, PBac{GH146-GAL4.B}-marked DL1 olfactory projection neurons mistarget their dendrites to the dorsolateral glomerulus DA41 in the anterior of the antennal lobe with 100% penetrance instead of the DL1 glomerulus in the posterior of the antennal lobe as in wild type. However, arborization of mutant axons is not obviously altered.

      PBac{GH146-GAL4.B}-marked anterodorsal projection neuron neuroblast clones in Bap55LL05955 mutants have a mild reduction in cell number and their dendrites are abnormally clustered in the anterior dorsal region of the antennal lobe, including the DA41 glomerulus.

      PBac{GH146-GAL4.B}-marked lateral projection neuron neuroblast clones in Bap55LL05955 mutants display a severe reduction in cell number and the remaining dendrites are unable to target to many glomeruli throughout the antennal lobe.

      PBac{GH146-GAL4.B}-marked ventral projection neuron neuroblast clones in Bap55LL05955 mutants display a mild reduction in cell number and a reduced dendrite mass throughout the antennal lobe.

      Expression of Bap55Scer\UAS.cTa using Scer\GAL4GH146.PB in a single cell clone in a Bap55LL05955 background results in a de novo phenotype in 2/21 cases: PBac{GH146-GAL4.B}-marked projection neuron dendrites target to neither DA41 nor DL1 glomeruli but to the DM6 glomerulus at the anterior medial region of the antennal lobe. In addition, the axon shows a mistargeting phenotype, extending ventrally to the lateral horn.

      (Tea and Luo, 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\ACTL6AScer\UAS.cTa or Hsap\ACTL6BScer\UAS.cTa using Scer\GAL4GH146.PB in a single cell clone rescues the DL1 dendrite mistargeting phenotype of Bap55LL05955 mutants in most cases. However, expression of Hsap\ACTL6AScer\UAS.cTa or Hsap\ACTL6BScer\UAS.cTa in 6/19 and 2/32 cases, respectively, causes a de novo projection neuron dendrite and axon mistargeting phenotype.

      (Tea and Luo, 2011)
      Complementation and Rescue Data
      Rescued by

      Bap55LL05955 is rescued by Bap55UAS.cTa/Scer\GAL4GH146.PB

      (Tea and Luo, 2011)
      Comments

      Expression of Bap55Scer\UAS.cTa using Scer\GAL4GH146.PB in a single cell clone fully rescues the DL1 dendrite mistargeting phenotype of Bap55LL05955 mutants in 19/21 cases.

      (Tea and Luo, 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Precise excision of the PBac{SAstopDsRed} element reverts the Bap55LL05955 dendrite mistargeting phenotype.

      (Tea and Luo, 2011)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Bap55-
      (Tea and Luo, 2011)
      Bap55LL05955
      (Tian and Smith-Bolton, 2021)
      LL05955
      (Tea and Luo, 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)