FlyBase Allele Report: Hsap\APP[Aβ42.UAS.cBa]

General Information

Symbol

Hsap\APP^{Aβ42.UAS.cBa}

Species

H. sapiens

Name

FlyBase ID

FBal0256662

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-APP.Aβ42.B}

Also Known As

UAS-Aβ42

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Singh and Mahoney, 2011.2.17)

Progenitor genotype

Carried in construct

P{UAS-APP.Aβ42.B}

Cytology

Description

UASt regulatory sequences drive expression of the 42 amino acid Aβ fragment from Hsap\APP tagged at the N-terminal end with a secretion signal peptide from aos.

(Chouhan et al., 2016)

UAS drives expression of the 42 amino acid Aβ fragment from Hsap\APP.

(Singh and Mahoney, 2011.2.17)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Wu et al., 2017, Yang et al., 2017, Chouhan et al., 2016, Lee et al., 2016)

model of Alzheimer's disease 1

CEA

(Mukhtar et al., 2024, Maggiore et al., 2022, Kim et al., 2021, Moulton et al., 2021, Nikookar et al., 2021, Wang and Davis, 2021, Abtahi et al., 2020, Haghi et al., 2020, Lee et al., 2020, Zhang et al., 2020, Hwang et al., 2019, Mattioli et al., 2019)

CEC

(Khatoon et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Alzheimer's disease

is exacerbated by sra^EY07182

(Lee et al., 2016)

is ameliorated by Hsap\CCN2^UAS.cYa

(Yang et al., 2017)

is ameliorated by Hsap\CCN2^repo.PY

(Yang et al., 2017)

model of Alzheimer's disease 1

is exacerbated by CG7896^KK105990

(Kim et al., 2021)

is ameliorated by CG5567^GD11694

(Kim et al., 2021)

is ameliorated by CG8888^EY12413

(Kim et al., 2021)

is ameliorated by CG6154^MI08916

(Kim et al., 2021)

is ameliorated by CG7137^GD12147

(Kim et al., 2021)

is ameliorated by Cont^GD12610

(Kim et al., 2021)

is exacerbated by Cox11^KK100158

(Kim et al., 2021)

is ameliorated by sro^GD7469

(Kim et al., 2021)

is ameliorated by Dgkε^ox-1

(Kim et al., 2021)

is exacerbated by E2f1^KK100304

(Kim et al., 2021)

is ameliorated by Hydr2^MI08405

(Kim et al., 2021)

is ameliorated by Thg^GD11217

(Kim et al., 2021)

is exacerbated by VhaM9.7-a^KK107389

(Kim et al., 2021)

is ameliorated by kek3^GD1733

(Kim et al., 2021)

is ameliorated by kek5^MI01444

(Kim et al., 2021)

is exacerbated by plx^KK100306

(Kim et al., 2021)

is ameliorated by Parp1^HMC04658

(Maggiore et al., 2022)

is ameliorated by Tip60^UAS.cLa

(Zhang et al., 2020)

is ameliorated by Acer^Δ168

(Lee et al., 2020)

is exacerbated by Pten^UAS.cUa

(Hwang et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage, with Scer\GAL4^elav-C155

(Wu et al., 2017)

abnormal jump response, with Scer\GAL4^elav.PLu

(Khatoon et al., 2019)

abnormal learning | third instar larval stage, with Scer\GAL4^elav-C155

(Zhang et al., 2020)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Zhang et al., 2020, Wu et al., 2017)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^elav.PU

(Yang et al., 2017)

abnormal locomotor behavior | day 15 of adulthood, with Scer\GAL4^elav.PLu

(Khatoon et al., 2019)

abnormal locomotor behavior | larval stage, with Scer\GAL4^elav-C155

(Zhang et al., 2020)

abnormal memory | adult stage | progressive, with Scer\GAL4^elav.PU

(Yang et al., 2017)

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav-C155

(Wu et al., 2017)

abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4^ninaE.PT

(Chouhan et al., 2016)

abnormal neuroanatomy | larval stage, with Scer\GAL4^GMR.PU

(Lee et al., 2016)

abnormal neurophysiology | adult stage, with Scer\GAL4^ninaE.PT

(Chouhan et al., 2016)

abnormal oxidative stress response | adult stage, with Scer\GAL4^elav-C155

(Wu et al., 2017)

abnormal short-term memory | adult stage, with Scer\GAL4^elav-C155

(Zhang et al., 2020)

abnormal short-term memory | third instar larval stage, with Scer\GAL4^elav-C155

(Zhang et al., 2020)

increased cell death | adult stage, with Scer\GAL4^elav.PLu

(Khatoon et al., 2019)

increased cell death | adult stage, with Scer\GAL4^elav-C155

(Zhang et al., 2020, Wu et al., 2017)

increased cell death | larval stage, with Scer\GAL4^GMR.PU

(Lee et al., 2016)

short lived, with Scer\GAL4^elav.PLu

(Khatoon et al., 2019)

short lived, with Scer\GAL4^elav.PU

(Mukhtar et al., 2024)

short lived, with Scer\GAL4^elav-C155

(Zhang et al., 2020, Wu et al., 2017)

visible | adult stage, with Scer\GAL4^GMR.PU

(Park et al., 2021, Lee et al., 2016)

Phenotype Manifest In

adult brain, with Scer\GAL4^elav.PU

(Yang et al., 2017)

adult brain, with Scer\GAL4^elav-C155

(Wu et al., 2017)

adult head, with Scer\GAL4^elav.PLu

(Khatoon et al., 2019)

adult Kenyon cell, with Scer\GAL4^elav-C155

(Zhang et al., 2020)

axon | third instar larval stage, with Scer\GAL4^GMR.PU

(Lee et al., 2016)

embryonic/larval brain, with Scer\GAL4^GMR.PU

(Lee et al., 2016)

embryonic/larval plasmatocyte | adult stage, with Scer\GAL4^elav-C155

(Wu et al., 2017)

eye, with Scer\GAL4^GMR.PU

(Park et al., 2021, Lee et al., 2016)

eye disc | larval stage, with Scer\GAL4^GMR.PU

(Lee et al., 2016)

eye photoreceptor cell | adult stage, with Scer\GAL4^ninaE.PT

(Chouhan et al., 2016)

mushroom body, with Scer\GAL4^elav-C155

(Zhang et al., 2020)

neuron | adult stage, with Scer\GAL4^elav.PU

(Yang et al., 2017)

ommatidium | adult stage | progressive, with Scer\GAL4^GMR.PU

(Yang et al., 2017)

photoreceptor neuron | larval stage, with Scer\GAL4^GMR.PU

(Lee et al., 2016)

rhabdomere | adult stage | progressive, with Scer\GAL4^GMR.PU

(Yang et al., 2017)

rhabdomere of eye photoreceptor cell | adult stage | progressive, with Scer\GAL4^ninaE.PT

(Chouhan et al., 2016)

Detailed Description

Statement

Reference

Expression of Hsap\APP^{Aβ42.UAS.cBa} under the control of Scer\GAL4^elav-C155 leads to diffuse amyloid deposits that do not appear in abundance in the mushroom body (MB) until flies are aged to at least 25 days.

Expression of Hsap\APP^{Aβ42.UAS.cBa} under the control of Scer\GAL4^elav-C155 leads to higher level of apoptotic neuronal cell death in the MB Kenyon cell region in the adult (28-days-old) brain.

Expression of Hsap\APP^{Aβ42.UAS.cBa} under the control of Scer\GAL4^elav-C155 leads to defects in both learning (in third instar larvae) and short term memory (in third instar larvae and adults).

Expression of Hsap\APP^{Aβ42.UAS.cBa} under the control of Scer\GAL4^elav-C155 leads to defects in larval locomotion (line crossing, righting, and contraction assays) and adult locomotion (negative geotaxis assay).

(Zhang et al., 2020)

Expression of Hsap\APP^{Aβ42.UAS.cBa} under the control of Scer\GAL4^elav.PLu leads to impaired jumping performance, late onset locomotory deficit (impaired negative geotaxis), increased oxidative stress (in terms of increased ROS level in mitochondria and reduction in mitochondrial membrane potential) and increase in mitochondrial mediated apoptosis when compared to control flies.

(Khatoon et al., 2019)

Adult flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^elav-C155 display a significant increase in vacuolar degeneration and increased apoptosis in the brain, decreased lifespan, show significant recruitment of plasmatocytes to the postero-dorsal site of the brain near the mushroom body lobe (although they do not infiltrate the brain and there is no significant increase in the total population of plasmatocytes), and a reduction in locomotor activity, as compared to controls. These phenotypes become more pronounced in response to infection with the pathogenic enterobacteria Ecc15 or Pseudomonas entomophila, whereas control flies do not exhibit any of these phenotypes upon Ecc15 or Pseudomonas infection. Flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^elav-C155 also show an increase in reactive oxygen species stress, this phenotype becomes more pronounced upon infection with Ecc15, whereas control flies do not exhibit any oxidative stress upon Ecc15 infection.

(Wu et al., 2017)

Expression of Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^elav.PU results in accumulation of Hsap\APP-positive puncta and neuronal loss in the adult brain and the flies also display memory loss (assayed under an olfactory associative learning paradigm) as well as age-progressive decrease in climbing ability, as compared to controls. Scer\GAL4^GMR.PU-driven expression leads to a progressive photoreceptor degeneration in the adult eye manifested by disorganization of ommatidial lattice and rhabdomere loss.

(Yang et al., 2017)

Expression of Hsap\APP^{Aβ42.Scer\UAS.cBa} driven by Scer\GAL4^ninaE.PT (crosses carried out at 18[o]C and shifted to 25[o]C following eclosion) does not significantly affect external eye morphology in adult flies. At 1 and 30 days old (but not 10), electroretinogram (ERG) recordings of light induced potentials from Scer\GAL4^ninaE.PT>Hsap\APP^{Aβ42.Scer\UAS.cBa} flies show a significant increase in light-evoked in depolarization amplitude compared to controls; mutant flies show significant increases in ON transient amplitude at day 1, 10 and 30 and a significant increase in OFF transient amplitude at day 1.

The number and morphology of photoreceptors (retinae show intact ommatidia with 7 visible rhabdomeres) in Scer\GAL4^ninaE.PT>Hsap\APP^{Aβ42.Scer\UAS.cBa} flies is grossly preserved at day 1, similar to controls; with aging (day 10 and 30), progressive vacuolar changes affect the retina and there is a significant decrease in rhabdomere numbers at day 30. Photoreceptor ultrastructure (examined with TEM) in 10 day old Scer\GAL4^ninaE.PT>Hsap\APP^{Aβ42.Scer\UAS.cBa} flies revealed significant subtle defects and irregularities in rhabdomeres (splitting or fraying at edges, vacuolar changes). Mutants at day 10 have similar numbers of mitochondria per ommatidium and percentage of photoreceptors with electron dense vacuoles (telolysosomes) as controls, but have a significant increase in the number of autophagic vacuoles (increased numbers of multivesicular or multilamellar bodies). Scer\GAL4^ninaE.PT>Hsap\APP^{Aβ42.Scer\UAS.cBa} flies do not show significant disruption of photoreceptor synaptic terminal organization and morphology at 10 days old compared to controls.

(Chouhan et al., 2016)

Expression of Hsap\APP^{Aβ42.Scer\UAS} under the control of Scer\GAL4^GMR.PU results in a severe small and rough-eye phenotype in adult flies, increased levels of cell death and neurodegeneration of photoreceptor neurons in larval eye disc as well as photoreceptor targeting defects in the larval brain.

(Lee et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, enhanceable by Cat^KK101528, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by CanA1^JF01871, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by CanB^JF02616, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by sra^EY07182, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has abnormal neuroanatomy | larval stage phenotype, enhanceable by sra^EY07182, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has increased cell death | larval stage phenotype, enhanceable by sra^EY07182, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by sra^UAS.cEa, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Suppressed by

Statement

Reference

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has increased cell death | adult stage phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has abnormal learning | third instar larval stage phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has abnormal short-term memory | third instar larval stage phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has abnormal short-term memory | adult stage phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has abnormal locomotor behavior | larval stage phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has increased cell death | adult stage phenotype, suppressible by egr^GD12658, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has increased cell death | adult stage phenotype, suppressible by egr^KK103432, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage phenotype, suppressible by egr^KK103432, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage phenotype, suppressible by egr^GD12658, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by egr^KK103432, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by egr^GD12658, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by dom^k08108/l(3)hem²

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has abnormal neuroanatomy phenotype, suppressible by dom^k08108/l(3)hem²

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav.PU has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by Hsap\CCN2^UAS.cYa, Scer\GAL4^elav.PU

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav.PU has abnormal memory | adult stage | progressive phenotype, suppressible by Hsap\CCN2^UAS.cYa, Scer\GAL4^elav.PU

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by sra^KO

(Lee et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has adult brain phenotype, enhanceable by Cat^KK101528, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has embryonic/larval plasmatocyte | adult stage phenotype, enhanceable by Cat^KK101528, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by sra^EY07182, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has eye disc | larval stage phenotype, enhanceable by sra^EY07182, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by CanA1^JF01871, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by CanB^JF02616, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has photoreceptor neuron | larval stage phenotype, enhanceable by sra^EY07182, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has axon | third instar larval stage phenotype, enhanceable by sra^EY07182, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has embryonic/larval brain phenotype, enhanceable by sra^EY07182, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by sra^UAS.cEa, Scer\GAL4^GMR.PU

(Lee et al., 2016)

Suppressed by

Statement

Reference

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has adult Kenyon cell phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has mushroom body phenotype, suppressible by Tip60^UAS.cLa, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has adult brain phenotype, suppressible by egr^KK103432, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has adult brain phenotype, suppressible by egr^GD12658, Scer\GAL4^elav-C155

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has adult brain phenotype, suppressible by dom^k08108/l(3)hem²

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155 has embryonic/larval plasmatocyte | adult stage phenotype, suppressible by dom^k08108/l(3)hem²

(Wu et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has ommatidium | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav.PU/Hsap\CCN2^UAS.cYa

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has rhabdomere | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav.PU/Hsap\CCN2^UAS.cYa

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has ommatidium | adult stage | progressive phenotype, suppressible by Hsap\CCN2^repo.PY

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has rhabdomere | adult stage | progressive phenotype, suppressible by Hsap\CCN2^repo.PY

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav.PU has adult brain phenotype, suppressible | partially by Hsap\CCN2^UAS.cYa, Scer\GAL4^elav.PU

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav.PU has neuron | adult stage phenotype, suppressible | partially by Hsap\CCN2^UAS.cYa, Scer\GAL4^elav.PU

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by sra^KO

(Lee et al., 2016)

NOT suppressed by

Statement

Reference

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has ommatidium | adult stage | progressive phenotype, non-suppressible by Mmp1^JF01336/Scer\GAL4^elav.PU/Hsap\CCN2^UAS.cYa

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has rhabdomere | adult stage | progressive phenotype, non-suppressible by Mmp1^JF01336/Scer\GAL4^elav.PU/Hsap\CCN2^UAS.cYa

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has ommatidium | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^elav.PU/Hsap\CCN2^UAS.cYa/Mmp2^JF01337

(Yang et al., 2017)

Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^GMR.PU has rhabdomere | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^elav.PU/Hsap\CCN2^UAS.cYa/Mmp2^JF01337

(Yang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of egr^GD12658 partially suppresses the increased cell death in the brain, the climbing defects, and the shortened lifespan seen in Ecc15-infected flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^elav-C155.

Co-expression of egr^KK103432 partially suppresses the increased cell death in the brain, the climbing defects, and the shortened lifespan seen in Ecc15-infected flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^elav-C155.

The presence of both l(3)hem² and dom^k08108 completely suppresses the shortened lifespan and recruitment of plasmatocytes to the postero-dorsal site of the brain near the mushroom body lobe, and partially suppresses the neurodegeneration seen in Ecc15 infected flies that express Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^elav-C155.

Co-expression of Cat^KK101528 leads to a significant increase in recruitment of plasmatocytes to the brain in flies that express Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^elav-C155.

(Wu et al., 2017)

The Hsap\APP protein accumulations and neuronal loss in adult brain along with the age-progressive memory loss and climbing ability deficit characteristic for adult flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^elav.PU can be at least partially rescued by co-expression of Hsap\CTGF^UAS.cYa.

Similarly, the progressive photoreceptor degeneration in the adult eye observed upon Scer\GAL4^GMR.PU-driven expression of Hsap\APP^{Aβ42.Scer\UAS.cBa} can be significantly suppressed either by co-expression of Hsap\CTGF^UAS.cYa or by combination with Hsap\CTGF^repo.PY (expressed in glial cells) but not by co-expression of Hsap\CTGF^UAS.cYa combined with either Mmp1^JF01336 or Mmp2^JF01337.

(Yang et al., 2017)

The severe rough eye phenotype in adults as well as the increased levels of cell death in the larval eye disc observed in flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PU are exacerbated further by co-expression of sra^EY07182.

The severe small and rough eye phenotype observed in flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PU is exacerbated further by co-expression of sra^Scer\UAS.cEa.

The severe small and rough eye phenotype observed in flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PU is significantly ameliorated by combination with sra^KO in heterozygous state.

The neurodegeneration of photoreceptor neurons in larval eye disc as well as the photoreceptor axon targeting defects in the brain observed in larvae expressing either Hsap\APP^{Aβ42.Scer\UAS} or sra^EY07182 under the control of Scer\GAL4^GMR.PU are significantly enhanced when the two transgenes are co-expressed.

The small and rough eye phenotype observed in flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PU is exacerbated further by co-expression of CanA1^JF01871 RNAi.

The small and rough eye phenotype observed in flies expressing Hsap\APP^{Aβ42.Scer\UAS.cBa} under the control of Scer\GAL4^GMR.PU is exacerbated further by co-expression of CanB^JF02616 RNAi.

(Lee et al., 2016)

Complementation and Rescue Data

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Mutant

Wild-type

Stocks (4)

Bloomington

33769

w¹¹¹⁸; P{UAS-APP.Aβ42.B}m26a

33771

P{ActGFP}38, w¹¹¹⁸; P{UAS-MAPT.VTR}12D P{UAS-APP.Aβ42.B}m26a