FlyBase Allele Report: Dmel\BubR1[KEN.mRFP1]

General Information

Symbol

Dmel\BubR1^KEN.mRFP1

Species

D. melanogaster

Name

FlyBase ID

FBal0247989

Feature type

allele

Associated gene

Dmel\BubR1

Associated Insertion(s)

Carried in Construct

P{BubR1-KEN.mRFP}

Also Known As

bubR1-KEN

Key Links

Transgenic product class

missense_variant

(Rahmani et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Rahmani et al., 2009)

Mutagen

in vitro construct

(Rahmani et al., 2009)

Progenitor genotype

BubR1^mRFP1

(Rahmani et al., 2009)

Carried in construct

P{BubR1-KEN.mRFP}

Cytology

Description

Amino acid replacement: K7A.

(Rahmani et al., 2009)

Two amino acids corresponding to the N-terminal KEN box of BubR1 are mutated in this transgene.

(Rahmani et al., 2009)

Amino acid replacement: E8A.

(Rahmani et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

BubR1

Encoded product / tool

BubR1

Tagged with

mRFP1

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Rahmani et al., 2009)

abnormal mitotic cell cycle | third instar larval stage, with BubR1^k06109

(Caous et al., 2015)

fertile

(Rahmani et al., 2009)

viable

(Rahmani et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

BubR1^{KEN.T:Disc\RFP-mRFP},BubR1^k06109 mutant larval brains (third instar) do not show any growth defects and have normal number of neuroblast as well as the mitotic index, the rate of aneu- or polyploid brain cells is however increased compared to controls.

(Caous et al., 2015)

Aneuploidy is at wild-type levels in BubR1^{KEN.T:Disc\RFP-mRFP} neuroblasts.

Colchicine fails to arrest BubR1^{KEN.T:Disc\RFP-mRFP} cells in mitosis and premature sister chromatid separation is approximately 20-fold higher than in wild-type.

BubR1^{KEN.T:Disc\RFP-mRFP} neuroblasts show no obvious defects in spindle structure or function. Stable spindles rapidly capture chromosomes and form robust K-fibers, often within 3 minutes of nuclear envelope breakdown, as in wild-type. Anaphase figures present normal symmetrical chromatids.

Mitotic timing is normal in BubR1^{KEN.T:Disc\RFP-mRFP} cells.

(Rahmani et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

BubR1^KEN.mRFP1, BubR1^k06109 has abnormal mitotic cell cycle | third instar larval stage phenotype, enhanceable by Sas-4^s2214/Sas-4^s2214

(Caous et al., 2015)

Enhancer of

Statement

Reference

BubR1^k06109/BubR1^KEN.mRFP1 is an enhancer of abnormal mitotic cell cycle | third instar larval stage phenotype of Sas-4^s2214

(Caous et al., 2015)

NOT Enhancer of

Statement

Reference

BubR1^k06109/BubR1^KEN.mRFP1 is a non-enhancer of neoplasia | third instar larval stage phenotype of aurA⁸⁸³⁹/aurA¹⁷⁹⁶¹

(Caous et al., 2015)

Suppressor of

Statement

Reference

BubR1^KEN.mRFP1 is a suppressor of abnormal mitotic cell cycle phenotype of asp^E3

(Rahmani et al., 2009)

Other

Statement

Reference

BubR1^KEN.mRFP1, Mps1^UAS.cAa, Scer\GAL4^{VP16.mat.αTub67C} has abnormal mitotic cell cycle | embryonic stage 5 phenotype

(Althoff et al., 2012)

BubR1^KEN.mRFP1, cnn^HK21 has lethal | pupal stage phenotype

(Rahmani et al., 2009)

BubR1^KEN.mRFP1, cnn^HK21 has abnormal mitotic cell cycle phenotype

(Rahmani et al., 2009)

BubR1^KEN.mRFP1, mad2^G6595 has abnormal mitotic cell cycle phenotype

(Rahmani et al., 2009)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

BubR1^k06109/BubR1^KEN.mRFP1 is a non-enhancer of larval neuroblast | third instar larval stage | increased number phenotype of aurA⁸⁸³⁹/aurA¹⁷⁹⁶¹

(Caous et al., 2015)

Suppressor of

Statement

Reference

BubR1^k06109/BubR1^KEN.mRFP1 is a suppressor of larval neuroblast | increased number | third instar larval stage phenotype of Sas-4^s2214

(Caous et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The increased neuroblast number and mitotic index seen in aurA⁸⁸³⁹/aurA¹⁷⁹⁶¹ transheterozygote larvae is not enhanced by combination with BubR1^{KEN.T:Disc\RFP-mRFP},BubR1^k06109 (the mitotic index in the double mutants is even slightly decreased), whereas the rate of ploidy defects is increased compared to either of the single gene mutants.

The increased brain neuroblast (NB) number observed in Sas-4^s2214 mutant third instar larvae is fully suppressed by combination with BubR1^{KEN.T:Disc\RFP-mRFP},BubR1^k06109 (the NB number in the double mutants is even lower than in wild-type controls and the brains are also smaller), while the rate of ploidy defects is strongly increased compared to either of the single gene mutants.

(Caous et al., 2015)

Co-expression of ald^Scer\UAS.cAa and BubR1^{KEN.T:Disc\RFP-mRFP} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in almost complete abolition of chromosome movement during anaphase. Spindle appearance is sudden but extension is limited. The width of the metaphase plate and sister kinetochore separation appear normal.

(Althoff et al., 2012)

The high mitotic density caused by asp^E3 is reduced five-fold in BubR1^{KEN.T:Disc\RFP-mRFP} asp^E3 double mutants.

Double mutants of BubR1^{KEN.T:Disc\RFP-mRFP} and cnn^HK21 are pupal lethal with aneuploidy averaging 15%.

BubR1^{KEN.T:Disc\RFP-mRFP} mad2^G6595 double mutants are viable and fertile, with aneuploidy levels no higher than in the single mutants. Anaphase figures also appear normal in double mutants. The time from nuclear envelope breakdown to anaphase is markedly reduced (by 40%) compared to BubR1^{KEN.T:Disc\RFP-mRFP} single mutant or wild-type cells and 30% reduced compared to mad2^G6595 single mutants. This acceleration is accompanied by an earlier onset of CycB breakdown. In contrast, the gap from CycB breakdown to anaphase is relatively constant between the double mutant and controls.

(Rahmani et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

BubR1^{KEN.T:Disc\RFP-mRFP}

BubR1^KEN.mRFP1

bubR1-KEN

(Caous et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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