Imprecise excision of P{EPgy2}trpmEY01618 that removes 2623 bp of the trpm sequence which includes exons 10-12 and about half of the adjacent introns.
Homozygotes die at the end of the larval stage, at the point of white prepupae formation.
Mutant animals show prolonged larval development, at the end of which they arrest and die at the prepupal stage. The mutant larvae have a lower body weight compared to controls throughout larval development, with the difference in weight being greatest at the end of the second larval instar.
Mutant animals lacking both maternal and zygotic function show an obvious growth defect by the end of the first larval instar, weighing less than controls. This defect progresses rapidly so that by the middle of the second instar they are approximately 17% the weight of controls. These larvae die approximately 48 hours after hatching.
The growth defect seen in mutant animals is underpinned by a reduction in cell size, whereas cell numbers and DNA replication are normal.
Mitochondria in mutant salivary glands are fused to form long tubular structures (in wild type they are seen as individual bullet-shaped structures).
Trpm28 is a suppressor of increased cell size phenotype of S6kSTDETE.UAS, Scer\GAL4AB1
Trpm28 is a suppressor of increased cell size phenotype of Pi3K92EUAS.Tag:MYC, Scer\GAL4AB1
Trpm28 is a suppressor of salivary gland phenotype of S6kSTDETE.UAS, Scer\GAL4AB1
Trpm28 is a suppressor of embryonic/larval salivary gland phenotype of Pi3K92EUAS.Tag:MYC, Scer\GAL4AB1
Expression of Pi3K92EScer\UAS.T:Hsap\MYC under the control of Scer\GAL4AB1 in a trpm28 background does not result in an increase in cell size in the larval salivary gland.
Expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4AB1 in a trpm28 background does not result in an increase in cell size in the salivary gland.
