FlyBase Allele Report: Dmel\Nlg1[ex2.3]

General Information

Symbol

Dmel\Nlg1^ex2.3

Species

D. melanogaster

Name

FlyBase ID

FBal0246577

Feature type

allele

Associated gene

Dmel\Nlg1

Associated Insertion(s)

Carried in Construct

Also Known As

dnlg1^ex2.3

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

FLPase

(Banovic et al., 2010)

recombination

(Banovic et al., 2010)

Progenitor genotype

PBac{WH}Nlg1^f00756

(Banovic et al., 2010)

P{XP}pyd3^d00812

(Banovic et al., 2010)

Cytology

Description

Recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them, which includes part of the Nlg1 gene.

(Banovic et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:7,679,179..7,750,087 [+]

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them.

(Banovic et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autism spectrum disorder

CEA

(Guangming et al., 2023)

CEA with Nlg1^ex1.9

(Sun et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage (with Df(3R)BSC747)

(Constance et al., 2018)

abnormal neuroanatomy | larval stage (with Nlg1^ex1.9)

(Sun et al., 2023)

abnormal neuroanatomy | larval stage (with Nlg1^I960)

(Mosca et al., 2012, Banovic et al., 2010)

abnormal neuroanatomy | pharate adult stage (with Df(3R)BSC747)

(Constance et al., 2018)

abnormal neuroanatomy | pupal stage P7 (with Df(3R)BSC747)

(Constance et al., 2018)

abnormal neuroanatomy | third instar larval stage (with Nlg1^ex1.9)

(Ramesh et al., 2021)

abnormal size | pharate adult stage (with Df(3R)BSC747)

(Constance et al., 2018)

decreased size | third instar larval stage (with Nlg1^ex1.9)

(Ramesh et al., 2021)

viable (with Df(3R)BSC747)

(Constance et al., 2018)

viable (with Nlg1^I960)

(Mosca et al., 2012)

Phenotype Manifest In

abdominal lateral tergosternal muscle cell | pharate adult stage (with Df(3R)BSC747)

(Constance et al., 2018)

abdominal lateral tergosternal muscle cell | pupal stage P7 (with Df(3R)BSC747)

(Constance et al., 2018)

adult motor neuron (with Df(3R)BSC747)

(Constance et al., 2018)

adult persistent motor neuron | pharate adult stage (with Df(3R)BSC747)

(Constance et al., 2018)

adult persistent motor neuron | pupal stage P7 (with Df(3R)BSC747)

(Constance et al., 2018)

distal axon | pharate adult stage (with Df(3R)BSC747)

(Constance et al., 2018)

distal axon | pupal stage P7 (with Df(3R)BSC747)

(Constance et al., 2018)

embryonic/larval neuromuscular junction | larval stage (with Nlg1^ex1.9)

(Sun et al., 2023)

embryonic/larval neuromuscular junction | third instar larval stage (with Nlg1^ex1.9)

(Ramesh et al., 2021)

muscle cell of A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Nlg1^ex1.9)

(Ramesh et al., 2021)

neuromuscular junction | larval stage (with Nlg1^I960)

(Banovic et al., 2010)

NMJ bouton (with Nlg1^I960)

(Mosca et al., 2012)

NMJ bouton | larval stage (with Nlg1^I960)

(Banovic et al., 2010)

presynaptic active zone (with Nlg1^ex1.9)

(Ramesh et al., 2021)

presynaptic active zone | decreased number (with Nlg1^ex1.9)

(Ramesh et al., 2021)

Detailed Description

Statement

Reference

Nlg1^ex1.9/Nlg1^ex2.3 third instar larvae show small muscle 4 NMJs, which present fewer but enlarged active zones, as compared to controls.

(Ramesh et al., 2021)

At 30-36 hours after puparium formation in Nlg1^ex2.3/Df(3R)BSC747 flies, persistent motor neurons which innervate the abdominal lateral tergosternal muscles have more tortuous axonal branches, but similarly dynamic filopodia compared to wild-type controls. In late pharate adults, axons display significantly reduced total arbor length and area of arbor coverage, but no differences between total branch numbers and arbor complexity compared to wild-type controls. Additionally, there is no difference in synaptic density in motor neuron axons in the adult.

Nlg1^ex2.3/Df(3R)BSC747 adults display a significantly reduced climbing speed and stunted motor neuron axonal arbors innervating the femur compared to wild-type controls.

(Constance et al., 2018)

Bouton number at the neuromuscular junction is reduced compared to controls in mutant Nlg1^ex2.3/Nlg1^I960 larvae.

(Mosca et al., 2012)

The neuromuscular junctions of Nlg1^ex2.3/Nlg1^I960 larvae are smaller than normal. The average bouton number per muscle area and average number of boutons normalized to synaptic branch length are significantly reduced, and the average diameter of the largest bouton at a given neuromuscular junction is slightly but significantly increased in the mutants.

(Banovic et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Df(1)Ten-a, Nlg1^I960/Nlg1^ex2.3 has lethal | larval stage phenotype

(Mosca et al., 2012)

Phenotype Manifest In