FlyBase Allele Report: Dmel\shi[ts.UAS.cUa]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Dmel\shi^ts.UAS.cUa

General Information

Symbol

Dmel\shi^ts.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0246565

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{UAS-shi.ts.U}

Also Known As

UAS-shi^TS, UAS-shi^ts1, UAS-shibire^ts, Shi^ts, UAS-shibire^ts1

Key Links

Transgenic product class

dominant_negative_variant

(Fricke et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Fricke et al., 2009)

Mutagen

in vitro construct

(Fricke et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-shi.ts.U}

Cytology

Description

UAS regulatory sequences drive expression of dominant negative form of shi.

(Fricke et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by zyd¹

(Weiss et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | adult stage | heat sensitive, with Hsap\RELA^AD.R14C08, Scer\GAL4^DBD.R14C08

(Lewis et al., 2015)

abnormal chemosensory behavior | adult stage | heat sensitive, with Hsap\RELA^AD.R14C08, Scer\GAL4^DBD.R15B01

(Lewis et al., 2015)

abnormal chemosensory behavior | third instar larval stage, with Scer\GAL4^c305a

(Hamid et al., 2021)

abnormal chemosensory behavior | third instar larval stage, with Scer\GAL4^Mef2.247

(Hamid et al., 2021)

abnormal chemosensory behavior | third instar larval stage, with Scer\GAL4^NP1131

(Hamid et al., 2021)

abnormal courtship behavior | :conditional | heat sensitive, with Scer\GAL4^VT032411

(Zhao et al., 2018)

abnormal feeding behavior | adult stage | :nutrition conditional | heat sensitive, with Scer\GAL4^SLC5A11.1.1

(Park et al., 2016)

abnormal learning | third instar larval stage, with Scer\GAL4^c305a

(Hamid et al., 2021)

abnormal learning | third instar larval stage, with Scer\GAL4^Mef2.247

(Hamid et al., 2021)

abnormal learning | third instar larval stage, with Scer\GAL4^NP1131

(Hamid et al., 2021)

abnormal locomotor rhythm | heat sensitive, with Scer\GAL4^upd1-E132

(Luo and Sehgal, 2012)

abnormal medium-term memory | adult stage | :heat sensitive | cold sensitive, with Scer\GAL4^G0050

(Muria et al., 2021)

abnormal medium-term memory | adult stage | :heat sensitive | conditional, with Scer\GAL4^c739

(Muria et al., 2021)

abnormal medium-term memory | adult stage | :heat sensitive | conditional, with Scer\GAL4^GMR44E04

(Muria et al., 2021)

abnormal medium-term memory | adult stage | :heat sensitive | conditional, with Scer\GAL4^GMR53A05

(Muria et al., 2021)

abnormal medium-term memory | adult stage | :heat sensitive | conditional, with Scer\GAL4^VT030559

(Muria et al., 2021)

abnormal medium-term memory | adult stage | :heat sensitive | conditional, with Scer\GAL4^VT048643

(Muria et al., 2021)

abnormal medium-term memory | adult stage | :heat sensitive | conditional, with Scer\GAL4^VT057244

(Muria et al., 2021)

abnormal medium-term memory | adult stage | :heat sensitive | conditional, with Scer\GAL4^VT064246

(Muria et al., 2021)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R30G08, Scer\GAL4^DBD.R48B03

(Yamagata et al., 2016)

abnormal memory | adult stage | heat sensitive, with Hsap\RELA^AD.R58E02, Scer\GAL4^DBD.R31F06

(Yamagata et al., 2016)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^AstA.2.1

(Yamagata et al., 2016)

abnormal neuroanatomy | heat sensitive, with Scer\GAL4^ey.3.5.Exel

(Yuva-Aydemir et al., 2011)

abnormal neuroanatomy | heat sensitive, with Scer\GAL4^repo.PU

(Yuva-Aydemir et al., 2011)

abnormal phototaxis | third instar larval stage, with Scer\GAL4^NP394

(Gong et al., 2010)

abnormal phototaxis | third instar larval stage, with Scer\GAL4^NP423

(Gong et al., 2010)

abnormal phototaxis | third instar larval stage, with Scer\GAL4^NP867

(Gong et al., 2010)

abnormal sleep, with Scer\GAL4^GMR56C01

(Stahl et al., 2018)

abnormal sleep, with Scer\GAL4^Mz709

(Stahl et al., 2018)

abnormal smell perception | adult stage | heat sensitive, with Scer\GAL4^NP0509

(Muria et al., 2021)

abnormal smell perception | adult stage | heat sensitive, with Scer\GAL4^NP7273

(Muria et al., 2021)

lethal | heat sensitive, with Scer\GAL4^da.G32

(Yuva-Aydemir et al., 2011)

lethal | heat sensitive, with Scer\GAL4^repo.PU

(Doherty et al., 2009)

some die during immature adult stage | recessive | heat sensitive, with Scer\GAL4^repo.PU

(Doherty et al., 2009)

visible | adult stage, with Scer\GAL4^elav.PU

(Guo et al., 2019)

visible | adult stage, with Scer\GAL4^Toll-6-D42

(Guo et al., 2019)

visible | heat sensitive, with Scer\GAL4^en-e16E

(Fricke et al., 2009)

Phenotype Manifest In

adult ensheathing glial cell | heat sensitive, with Scer\GAL4^Mz709

(Doherty et al., 2009)

eye disc | heat sensitive, with Scer\GAL4^ey.3.5.Exel

(Yuva-Aydemir et al., 2011)

eye disc | heat sensitive, with Scer\GAL4^repo.PU

(Yuva-Aydemir et al., 2011)

glial cell | heat sensitive, with Scer\GAL4^ey.3.5.Exel

(Yuva-Aydemir et al., 2011)

glial cell | heat sensitive, with Scer\GAL4^repo.PU

(Yuva-Aydemir et al., 2011)

wing, with Scer\GAL4^elav.PU

(Guo et al., 2019)

wing, with Scer\GAL4^Toll-6-D42

(Guo et al., 2019)

wing hair | increased number | heat sensitive, with Scer\GAL4^en-e16E

(Fricke et al., 2009)

Detailed Description

Statement

Reference

Expression of shi^{ts.Scer\UAS.cUa} under the control of Scer\GAL4^upd-E132 at 29[o]C (restrictive temperature) lengthens the period of locomotor activity by approximately 0.7 hours under constant darkness conditions. Locomotor activity rhythms revert back to normal after the flies are shifted to 21[o]C (permissive temperature).

(Luo and Sehgal, 2012)

Expression of shi^{ts.Scer\UAS.cUa} under the control of either Scer\GAL4^ey.3.5.Exel or Scer\GAL4^repo.PU at the restrictive temperature results in a glial overmigration phenotype in the eye disc.

(Yuva-Aydemir et al., 2011)

Expression of shi^{ts.Scer\UAS.cUa} in early to mid-third instar larvae under the control of any one of Scer\GAL4^NP423, Scer\GAL4^NP867 or Scer\GAL4^NP394 leads to positive larval phototaxis at the restrictive temperature, whereas controls show negative phototaxis.

(Gong et al., 2010)

Animals carrying shi^{ts.Scer\UAS.cUa} and Scer\GAL4^repo.PU raised at 18[o]C and then subsequently shifted as adults to 30[o]C show 100% lethality within 3 days of the temperature shift.

Expression of shi^{ts.Scer\UAS.cUa} under the control of Scer\GAL4^Mz709 at 30[o]C strongly suppresses glial clearance of axonal debris from the central nervous system after severing of olfactory receptor neurons. Expression of shi^{ts.Scer\UAS.cUa} under the control of Scer\GAL4^alrm.PD at 30[o]C has no effect on axon debris clearance after severing of olfactory receptor neurons.

(Doherty et al., 2009)

Animals carrying shi^{ts.Scer\UAS.cUa} expressed under the control of Scer\GAL4^en-e16E and shifted to the restrictive temperature of 31[o]C at the third larval instar show a dramatic induction of wing hairs. If the shift to the restrictive temperature occurs at 26 hours after puparium formation, a milder wing-hair duplication phenotype is seen in the resulting adults.

(Fricke et al., 2009)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Shi^ts

(Boto et al., 2019, Weiss et al., 2019)

shi^{ts.Scer\UAS.cUa}

shi^ts.UAS.cUa

Name Synonyms

Secondary FlyBase IDs

References (44)

Report Sections