FlyBase Allele Report: Dmel\Rdl[RNAi.8-10.UAS]

General Information

Symbol

Dmel\Rdl^{RNAi.8-10.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0243638

Feature type

allele

Associated gene

Dmel\Rdl

Associated Insertion(s)

Carried in Construct

P{UAS-Rdl.RNAi.8-10}

Also Known As

UAS-Rdl-RNAi, Rdli8-10

Key Links

Transgenic product class

RNAi_reagent

(Liu et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Liu et al., 2007)

Mutagen

in vitro construct

(Liu et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Rdl.RNAi.8-10}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a fragment encoding Rdl exons 8 to 10.

(Liu et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rdl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior, with Scer\GAL4^elav.PU

(Yuan et al., 2014)

abnormal behavior | larval stage, with Scer\GAL4^elav.PU

(Liu et al., 2014)

abnormal circadian behavior | adult stage, with Scer\GAL4^P2.4.Pdf

(Parisky et al., 2008)

abnormal learning, with Scer\GAL4^c739

(Liu et al., 2007)

abnormal learning, with Scer\GAL4^c747

(Liu et al., 2009, Liu et al., 2007)

abnormal sleep, with Scer\GAL4^c305a

(Haynes et al., 2015)

lethal, with Scer\GAL4^elav-C155

(Liu et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Rdl^{dsRNA.8-10.Scer\UAS} under the control of Scer\GAL4^c305a significant loss of nighttime sleep that is the result of a decrease in the duration of nighttime sleep episodes, although this is partially compensated for by an increase in the number of nighttime sleep episodes.

(Haynes et al., 2015)

Expression of Rdl^{dsRNA.8-10.Scer\UAS} under the control of Scer\GAL4^elav.PU significantly increases green-light avoidance in larvae compared to controls.

(Liu et al., 2014)

Pan-neuronal expression of Rdl^{dsRNA.8-10.Scer\UAS} under the control of Scer\GAL4^elav.PU severely impairs the inhibition of female contact-induced male-male aggression seen in wild type males that have previously been exposed to females.

(Yuan et al., 2014)

Scer\GAL4^c747-mediated expression of Rdl^{dsRNA.8-10.Scer\UAS} results in significantly enhanced learning after aversive olfactory conditioning (flies trained to associate odors with an electric shock).

Flies expressing Rdl^{dsRNA.8-10.Scer\UAS} under the control of Scer\GAL4^c747 show improved learning performance compared to controls after appetitive olfactory conditioning with 0.5M and 1.0M sucrose presented with a conditioned odor. The duration of starvation before the assay does not alter the enhanced learning performance of these flies. These flies show normal responses to odors and sucrose, indicating the phenotype is not caused by defective sensation.

(Liu et al., 2009)

Knockdown of Rdl using Rdl^{dsRNA.8-10.Scer\UAS} driven by Scer\GAL4^P2.4.Pdf results in a small decrease in sleep and a small increase in sleep latency, during day and night.

(Parisky et al., 2008)

Scer\GAL4^c747/+;Rdl^{dsRNA.8-10.Scer\UAS}/+ flies exhibit a 10%-20% enhanced performance in memory acquisition tests compared to controls, when trained with a 1 minute odour exposure paired with 1, 3 or 12 shocks. A similar enhanced performance is also seen in flies expressing Rdl^{dsRNA.8-10.Scer\UAS} with Scer\GAL4^c739.

(Liu et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rdl^{RNAi.8-10.UAS}, Scer\GAL4^GH146 is an enhancer of abnormal smell perception | heat sensitive phenotype of Scer\GAL4^GH146, TrpA1^UAS.(B).cKa

(Sudhakaran et al., 2012)

Rdl^{RNAi.8-10.UAS}, Scer\GAL4^VPN is an enhancer of abnormal smell perception | heat sensitive phenotype of Scer\GAL4^VPN, TrpA1^UAS.(B).cKa

(Sudhakaran et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^c747/Rdl^{RNAi.8-10.UAS} is a suppressor of paralytic | recessive phenotype of kcc^DHS1

(Hekmat-Scafe et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^c747/Rdl^{RNAi.8-10.UAS} is a non-suppressor of abnormal learning phenotype of rut²⁰⁸⁰

(Liu et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rdl^{dsRNA.8-10.Scer\UAS} in projection neurons expressing TrpA1^{Scer\UAS.(B).cKa} (under the control of either Scer\GAL4^GH146 or Scer\GAL4^VPN) blocks habituation induced by exposure to 29[o]C for 30 minutes.

(Sudhakaran et al., 2012)

The bang-sensitive paralytic phenotype of homozygous kcc^DHS1 mutants is suppressed to great extent by Scer\GAL4^c747-driven Rdl^{dsRNA.8-10.Scer\UAS} expression.

(Hekmat-Scafe et al., 2010)

Scer\GAL4^c747-mediated expression of Rdl^{dsRNA.8-10.Scer\UAS} does not suppress the low learning performance of rut²⁰⁸⁰ flies after aversive olfactory conditioning.

(Liu et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

89903

w¹¹¹⁸; P{UAS-Rdl.RNAi.8-10}G

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rdl^{RNAi.8-10.UAS}

Rdl^{dsRNA.8-10.Scer\UAS}

Rdl^{dsRNA.8-10.UAS}

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

Open Close