FlyBase Allele Report: Dmel\mi[ΔEY22]

General Information

Symbol

Dmel\mi^ΔEY22

Species

D. melanogaster

Name

FlyBase ID

FBal0242036

Feature type

allele

Associated gene

Dmel\mi

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

FLPase

Nature of the Allele

Allele class

loss of function allele

(Szuplewski et al., 2009)

Mutagen

FLPase

(Szuplewski et al., 2009)

Progenitor genotype

P{EPgy2}mi^EY01258

(Szuplewski et al., 2009)

Cytology

Description

A deletion that removes the first two exons and part of the third exon. Generated by imprecise excision of P{EPgy2}mi^EY01258.

(Szuplewski et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cell & larval salivary gland | somatic clone

(Szuplewski et al., 2009)

macrochaeta & thorax (with mi¹)

(Szuplewski et al., 2009)

nucleus & cell & larval salivary gland | somatic clone

(Szuplewski et al., 2009)

wing disc

(Szuplewski et al., 2009)

Detailed Description

Statement

Reference

Homozygotes die mainly during early larval stages. Survivors show a developmental delay and reduced growth rate. After 5 days, the largest mutant larvae are much smaller than controls. By 11 days, the surviving mutants that have pupated are also small.

In a wild type background, mi^ΔEY22 clones in the wing imaginal disc are eliminated. mi^ΔEY22 clones in a Minute background survive and cells accumulate in G2/M phase at the expense of cells in G1.

Cells from mi^ΔEY22 wing imaginal discs are shifted towards G2/M phase at the expense of cells in G1 phase, compared to controls.

Homozygous imaginal wing discs are small with elevated levels of cell death.

mi^ΔEY22 salivary gland cells are small with small nuclei.

mi¹/mi^ΔEY22 flies show a small bristle phenotype on the adult dorsal thorax.

(Szuplewski et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

mi¹/mi^ΔEY22 has visible phenotype, non-enhanceable by Cdk2²/cdc2c[+]

(Szuplewski et al., 2009)

mi¹/mi^ΔEY22 has visible phenotype, non-enhanceable by Cdk2³/cdc2c[+]

(Szuplewski et al., 2009)

mi^ΔEY22 has decreased cell size | somatic clone phenotype, non-enhanceable by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Szuplewski et al., 2009)

Suppressed by

Statement

Reference

mi¹/mi^ΔEY22 has visible phenotype, suppressible by CycE^AR95/CycE[+]

(Szuplewski et al., 2009)

mi^ΔEY22 has FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype, suppressible | partially by CycE^AR95/CycE[+]

(Szuplewski et al., 2009)

mi¹/mi^ΔEY22 has visible phenotype, suppressible by CycE[+]/CycE⁵

(Szuplewski et al., 2009)

NOT suppressed by

Statement

Reference

mi¹/mi^ΔEY22 has visible phenotype, non-suppressible by Cdk2²/cdc2c[+]

(Szuplewski et al., 2009)

mi¹/mi^ΔEY22 has visible phenotype, non-suppressible by Cdk2³/cdc2c[+]

(Szuplewski et al., 2009)

mi^ΔEY22 has decreased cell size | somatic clone phenotype, non-suppressible by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Szuplewski et al., 2009)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

mi¹/mi^ΔEY22 has macrochaeta & thorax phenotype, non-enhanceable by Cdk2²/cdc2c[+]

(Szuplewski et al., 2009)

mi¹/mi^ΔEY22 has macrochaeta & thorax phenotype, non-enhanceable by Cdk2³/cdc2c[+]

(Szuplewski et al., 2009)

mi^ΔEY22 has cell & larval salivary gland | somatic clone phenotype, non-enhanceable by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Szuplewski et al., 2009)

mi^ΔEY22 has nucleus & cell & larval salivary gland | somatic clone phenotype, non-enhanceable by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Szuplewski et al., 2009)

Suppressed by

Statement

Reference

mi¹/mi^ΔEY22 has macrochaeta & thorax phenotype, suppressible by CycE[+]/CycE⁵

(Szuplewski et al., 2009)

mi¹/mi^ΔEY22 has macrochaeta & thorax phenotype, suppressible by CycE^AR95/CycE[+]

(Szuplewski et al., 2009)

NOT suppressed by

Statement

Reference

mi¹/mi^ΔEY22 has macrochaeta & thorax phenotype, non-suppressible by Cdk2²/cdc2c[+]

(Szuplewski et al., 2009)

mi¹/mi^ΔEY22 has macrochaeta & thorax phenotype, non-suppressible by Cdk2³/cdc2c[+]

(Szuplewski et al., 2009)

mi^ΔEY22 has cell & larval salivary gland | somatic clone phenotype, non-suppressible by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Szuplewski et al., 2009)

mi^ΔEY22 has nucleus & cell & larval salivary gland | somatic clone phenotype, non-suppressible by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Szuplewski et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for either CycE⁵ or CycE^AR95 suppresses the small bristle phenotype of mi¹/mi^ΔEY22 flies, whereas heterozygosity for cdc2c² or cdc2c³ has no effect.

Many more mi^ΔEY22 clones are recovered from imaginal wing discs in a CycE^AR95/+ background than in a wild type background.

(Szuplewski et al., 2009)

Xenogenetic Interactions

Statement

Reference

mi^ΔEY22 salivary gland cells remain small with small nuclei when BacA\p35^Scer\UAS.cHa is expressed under the control of Scer\GAL4^tub.

(Szuplewski et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mi^ΔEY22

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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