FB2024_03 , released June 25, 2024
Allele: Dmel\AGO2V966M
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General Information
Symbol
Dmel\AGO2V966M
Species
D. melanogaster
Name
FlyBase ID
FBal0241950
Feature type
allele
Associated gene
Dmel\AGO2
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Kim et al., 2007)
Progenitor genotype
Cytology
Description

Amino acid replacement: V966M.

(Kim et al., 2007)

The amino acid replacement mutates the GxDV motif into GxDM, potentially disrupting the coordination of the metal ion required for RNA cleavage actibity.

(Kim et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:15,559,829..15,559,829 [+]
Nucleotide change:

G15559829A

Amino acid change:

V966M | AGO2-PB; V969M | AGO2-PC; V539M | AGO2-PE

Reported amino acid change:

V966M

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Kim et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      AGO2V966M mutant lysate shows a two-fold reduced rate of siRNA unwinding while the rate of passenger strand cleavage is also strongly reduced.

      (Kim et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      AGO2414

      (Kim et al., 2007)
      Rescued by

      AGO2V966M is rescued by AGO2+t10.1

      (Kim et al., 2007)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      AGO2V966M
      (Eyk et al., 2019)
      Ago2V966M
      (Lee et al., 2020, Kim et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)