FlyBase Allele Report: Dmel\AMPKα[1]

General Information

Symbol

Dmel\AMPKα¹

Species

D. melanogaster

Name

FlyBase ID

FBal0241419

Feature type

allele

Associated gene

Dmel\AMPKα

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Swick et al., 2013)

Mutagen

ethyl methanesulfonate

(Swick et al., 2013)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:1,377,048..1,377,048 [+]

Nucleotide change:

C1377048T

Amino acid change:

S211L | AMPKalpha-PA; S211L | AMPKalpha-PB; S211L | AMPKalpha-PC

Reported amino acid change:

S211L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Swick et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

centrosome

(Andersen et al., 2012)

dendritic arborizing neuron | second instar larval stage

(Swick et al., 2013)

follicle cell

(Haack et al., 2013)

neuroblast

(Andersen et al., 2012)

spindle

(Andersen et al., 2012)

Detailed Description

Statement

Reference

Hemizygous AMPKα¹ males do not survive to adulthood.

(Onyenwoke et al., 2015)

AMPKα¹ follicle cell clones show normal polarity under both well-fed and starvation conditions. Polarity is also maintained after feeding flies drugs that reduce cellular ATP levels and activate AMPK, though many egg chambers degenerate at stage 7/8.

Cells within AMPKα¹ follicle clones are larger than adjacent wild type cells, under both fed and starved conditions.

(Haack et al., 2013)

Homozygous mutants display a completely penetrant phenotype of significantly enlarged plasma membrane domains in dendrites but not in axonal compartments of da neurons in second instar larvae.

(Swick et al., 2013)

SNF1A¹ mutant neuroblasts show a loss of apical protein localisation at prophase, slower cell cycle progression, cytokinesis failure, polyploidy, multiple centrosomes and malformed spindles, although scrib localisation is as in wild-type.

(Andersen et al., 2012)

Mutant larvae are decreased in size compared to controls.

Homozygous clones of cells in the larval fat body are equal in size to the surrounding heterozygous cells.

Mutant third instar larvae transferred to dyed food show a similar amount of dyed food in their gut as control larvae after 24 hours feeding. However, the mutant larvae show a defect in gut clearance, with significant amounts of dyed food remaining in their guts even 24 hours after removal of the dyed food (most wild-type larvae clear the dyed food from their guts within 10 hours).

(Bland et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

AMPKα¹ has lethal - all die during P-stage | recessive phenotype, suppressible by Scer\GAL4^Tub.PU/S6k^KQ.UAS

(Onyenwoke et al., 2015)

AMPKα¹ has lethal - all die during P-stage | recessive phenotype, suppressible by Trpml¹

(Onyenwoke et al., 2015)

AMPKα¹ has lethal - all die during P-stage | recessive phenotype, suppressible by Trpml²

(Onyenwoke et al., 2015)

AMPKα¹ has lethal - all die during P-stage | recessive phenotype, suppressible by Scer\GAL4^Act.PU/Trpml^JF01239

(Onyenwoke et al., 2015)

NOT suppressed by

Statement

Reference

AMPKα¹ has lethal - all die during P-stage | recessive phenotype, non-suppressible by Scer\GAL4^Tub.PU/mTor^TED.UAS

(Onyenwoke et al., 2015)

AMPKα¹ has lethal - all die during P-stage | recessive phenotype, non-suppressible by Trpml^UAS.cVa/Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

AMPKα¹ has lethal - all die during P-stage | recessive phenotype, non-suppressible by SNF4Aγ^UAS.GFP/Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The pupal-stage lethality of hemizygous AMPKα¹ males can be partially rescued by expression of S6k^KQ.Scer\UAS under the control of Scer\GAL4^tub.PU but not by expression of either of the following: Tor^TED.Scer\UAS, Trpml^Scer\UAS.cVa, SNF4Aγ^{Scer\UAS.T:Avic\GFP}.

The pupal-stage lethality of hemizygous AMPKα¹ males can be partially rescued by combination with either Trpml¹ or Trpml².

The pupal-stage lethality of hemizygous AMPKα¹ males can be partially rescued by expression of Trpml^JF01239 RNAi under the control of Scer\GAL4^Act.PU.

(Onyenwoke et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

AMPKα¹ is rescued by Scer\GAL4^Ubi.PU/AMPKα^UAS.mCherry

(Swick et al., 2013)

AMPKα¹ is rescued by AMPKα^UAS.cMa/Scer\GAL4^Ubi.PU

(Swick et al., 2013)

Partially rescued by

AMPKα¹ is partially rescued by AMPKα^UAS.cMa/Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

Comments

The pupal stage lethality observed in either AMPKα¹ or AMPKα³ hemizygous males can be partially rescued by expression of AMPKα^Scer\UAS.cMa under the control of Scer\GAL4^tub.PU.

(Onyenwoke et al., 2015)

Scer\GAL4^Ubi-mediated expression of SNF1A^{Scer\UAS.T:Disc\RFP-mCherry} rescues viability and fertility in SNF1A¹ mutants.

Scer\GAL4^Ubi-mediated expression of SNF1A^Scer\UAS.cMa rescues viability in SNF1A¹ mutants.

(Swick et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33830

AMPKα¹/FM7i

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

AMPKα¹

(Andersen et al., 2012)

SNF1A¹

(Onyenwoke et al., 2015)

ampkα¹

(Haack et al., 2013, Swick et al., 2013)

dAMPKα¹

(Bland et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (5)

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