FB2024_03 , released June 25, 2024
Allele: Dmel\AMPKαUAS.cMa
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General Information
Symbol
Dmel\AMPKαUAS.cMa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Mirouse
FlyBase ID
FBal0241422
Feature type
allele
Associated gene
Dmel\AMPKα
Associated Insertion(s)
Carried in Construct
P{UAS-AMPKα.M}
Also Known As
UAS-AMPKα, UAS-AMPK
Key Links
Transgenic product class
wild_type
(Swick et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Swick et al., 2013)
Mutagen
in vitro construct
(Swick et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-AMPKα.M}
Cytology
Description

UASt regulatory sequences drive expression of the AMPKα-RA isoform.

(Swick et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
AMPKα
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  congenital muscular dystrophy due to LMNA mutation
      modeled by LamCG489V.UAS
      (Chandran et al., 2019)
      ameliorates  NGLY1-deficiency
      modeled by Pnglex14
      (Han et al., 2020)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of AMPKαUAS.cMa under the control of Scer\GAL4GSG5966 (and RU486 feeding) leads to a significant decrease in lipid droplets (ie number, size and relative cellular content) in the adult midgut, as compared to controls.

      The expression of AMPKαUAS.cMa under the control of Scer\GAL4hs.PB and induced by shift to 29[o]C for 2 days before the experiment induces lipid droplet autophagy (dual LS2- and Atg8-positive puncta) in the adult midgut, as compared to controls.

      (Lee et al., 2018)

      Expression of one or two copies of SNF1AK57A.Scer\UAS under the control of Scer\GAL4Ubi does not significantly reduce adult lifespan.

      Ubiquitous expression of SNF1AScer\UAS.cMa (under the control of Scer\GAL4Ubi) does not affect levels of locomotion.

      (Johnson et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of LamCUAS.cSa and AMPKαUAS.cMa under the control of Scer\GAL4fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

      (Chandran et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      AMPKαUAS.cMa/Scer\GAL4Tub.PU rescues AMPKαJF01951

      (Onyenwoke et al., 2015)

      AMPKαUAS.cMa/Scer\GAL4Ubi.PU rescues AMPKα1

      (Swick et al., 2013)

      Scer\GAL4109(2)80/AMPKαUAS.cMa rescues AMPKα3

      (Swick et al., 2013)

      AMPKαUAS.cMa/Scer\GAL4Ubi.PU rescues AMPKα3

      (Swick et al., 2013)

      Scer\GAL4109(2)80/AMPKαUAS.cMa rescues AMPKα3

      (Kazgan et al., 2010)
      Partially rescues

      AMPKαUAS.cMa/Scer\GAL4Tub.PU partially rescues AMPKα1

      (Onyenwoke et al., 2015)

      AMPKαUAS.cMa/Scer\GAL4Tub.PU partially rescues AMPKα3

      (Onyenwoke et al., 2015)
      Comments

      The late pupal lethality observed in animals expressing AMPKαJF01951 under the control of Scer\GAL4tub.PU can be fully rescued by co-expression of AMPKαScer\UAS.cMa.

      The pupal stage lethality observed in either AMPKα1 or AMPKα3 hemizygous males can be partially rescued by expression of AMPKαScer\UAS.cMa under the control of Scer\GAL4tub.PU.

      (Onyenwoke et al., 2015)

      Scer\GAL4Ubi-mediated expression of SNF1AScer\UAS.cMa rescues viability in SNF1A1 and SNF1A3 mutants.

      Scer\GAL4109(2)80-mediated expression of SNF1AScer\UAS.cMa rescues the dendritic phenotype of SNF1A3 mutants.

      (Swick et al., 2013)

      Expression of SNF1AScer\UAS.cMa under the control of Scer\GAL4109(2)80 rescues the neuronal defects of SNF1A3 mutants.

      (Kazgan et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      AMPKαScer\UAS.cMa
      AMPKαUAS.cMa
      SNF1AScer\UAS.cMa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct of Mirouse
      Secondary FlyBase IDs
        References (8)