FB2024_03 , released June 25, 2024
Allele: Dmel\Syx18UAS.cGa
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General Information
Symbol
Dmel\Syx18UAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Gorczyca
FlyBase ID
FBal0241370
Feature type
allele
Associated gene
Dmel\Syx18
Associated Insertion(s)
Carried in Construct
P{UAS-Syx18.G}
Key Links
Transgenic product class
cDNA
(Gorczyca et al., 2007)
wild_type
(Gorczyca et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Gorczyca et al., 2007)
wild_type
(Gorczyca et al., 2007)
Mutagen
in vitro construct
(Gorczyca et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-Syx18.G}
Cytology
Description

UASt sequences control expression of a full length Syx18 cDNA.

(Gorczyca et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Syx18
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Larvae expressing Syx18Scer\UAS.cGa under the control of Scer\GAL4C57 have normal muscle size.

      Expression of Syx18Scer\UAS.cGa under the control of Scer\GAL4C57 results in altered neuromuscular junction (NMJ) morphology in third instar larvae, and a decreased number of boutons per muscle area at the NMJ.

      Marker expression indicates that the volume of subsynaptic reticulum (SSR) underlying NMJs is reduced when Syx18Scer\UAS.cGa is driven with Scer\GAL4C57. However, ectopic SSR-like structures are formed away from the NMJ and large ectopic vesicles form in the cytoplasm of muscle cells.

      (Gorczyca et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4C57, Syx18UAS.cGa has subsynaptic reticulum | ectopic phenotype, non-suppressible by dlg17

      (Gorczyca et al., 2007)
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The ectopic subsynaptic reticulum-like structures seen in muscles expressing Syx18Scer\UAS.cGa under the control of Scer\GAL4C57 are not suppressed by dlg17.

      The expansion of the subsynaptic reticulum of larval neuromuscular junctions due to dlg1Scer\UAS.cBa; Scer\GAL4BG487 is partially suppressed by co-expression of Syx18Scer\UAS.cGa.

      (Gorczyca et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Syx18UAS.cGa/Scer\GAL4T80 partially rescues Syx18ex6

      (Gorczyca et al., 2007)

      Syx18UAS.cGa/Scer\GAL4C57 partially rescues Syx18ex6

      (Gorczyca et al., 2007)
      Fails to rescue

      Scer\GAL4futsch-C380/Syx18UAS.cGa fails to rescue Syx18ex6

      (Gorczyca et al., 2007)
      Comments

      Expression of Syx18Scer\UAS.cGa under the control of Scer\GAL4T80 rescues the lethality, defects in bouton morphology and decrease in muscle size that is seen in Syx18ex6 mutants.

      Expression of Syx18Scer\UAS.cGa under the control of Scer\GAL4T80 does not rescue the decrease in evoked excitatory junctional potential (EJP) amplitude seen in Syx18ex6 mutants, but does rescue the EJP decay time constant and the miniature excitatory junctional potential (mEJP) amplitude in these animals. The frequency of mEJPs is almost rescued.

      Expression of Syx18Scer\UAS.cGa under the control of Scer\GAL4C57 rescues the lethality and decrease in muscle size that is seen in Syx18ex6 mutants. However, no rescue of the bouton phenotypes is seen in these animals.

      (Gorczyca et al., 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Syx18Scer\UAS.cGa
      Syx18UAS.cGa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Gorczyca
      Secondary FlyBase IDs
        References (1)