Imprecise excision of the progenitor insertion, resulting in a 4162bp deletion spanning from 2100bp 5' to 2059bp 3' of the translation start codon. The deletion removes the coding sequence for amino acid residues 1-687.
4162bp deletion from 2100bp 5' to 2059bp 3' of the translation start codon resulting from the imprecise excision of P{EP}GE20158.
actin filament & follicle cell
kug58D mutant follicle cells in mosaic egg chambers fail to migrate and exhibit a substantial loss of actin-rich protrusions at the basal follicle surface, and abnormal distribution of filopodia, as compared to wild type controls.
Egg chambers of homozygous females fail to elongate and remain almost spherical until stage 14 (this correlates with the failure of follicle cells to elongate along their anteroposterior axis). The mutant stage 14 egg chambers have abnormally short dorsal appendages.
Egg chambers of kug103C/kug58D females are not as elongated as wild-type egg chambers at stage 9, but the oocyte nucleus migrates normally to the dorsal anterior corner of the oocyte.
The number of pairs of polar follicle cells is normal in kug58D/Df(3L)BSC2 egg chambers.
Actin filaments are still parallel within cells in homozygous follicle cells. However, in contrast to controls, the actin filaments are no longer strictly oriented perpendicular to the anteroposterior axis of stage 8 or stage 12 homozygous egg chambers. The actin filaments do not appear to be randomly oriented, but are frequently oriented in parallel in neighbouring cells.
Homozygous female germline clones result in 14.5% spherical stage 14 egg chambers. This occasional formation of spherical egg chambers may be due to the occasional formation of large mutant clones in the follicle epithelium that are inevitably generated in parallel with the germline clones.
Large homozygous clones in the follicular epithelium frequently result in spherical egg chambers. Mosaic egg chambers in which fewer than approximately 60% of all follicle cells are mutant have a shape that is indistinguishable from that of control egg chambers, irrespective of the position these clones occupy within the follicle epithelium. Actin filaments are normally oriented in these mosaic clones. Mosaic egg chambers in which more than approximately 60% of all follicle cells are mutant are spherical in shape, irrespective of the position these clones occupy within the follicle epithelium. Basal actin filaments are no longer properly oriented perpendicular to the anteroposterior axis of the egg chamber in these clones. Actin filaments are no longer properly oriented in the remaining control cells in the mosaic egg chamber, irrespective of whether the control cells are located immediately adjacent to homozygous cells or not.
kug58D is partially rescued by kugΔICR.EGFP
kug58D is not rescued by kugΔTMICR.EGFP
Expression of kugT:Avic\GFP in kug58D mutants restores normal elongation and rotation of egg chambers as well as normal oviposition. It also rescues the disrupted subcellular localization of actin, microtubule and collagen IV fibers in the extracellular matrix of the follicle epithelium.
Expression of kugΔICR.T:Avic\GFP-EGFP in kug58D mutants restores normal elongation of egg chambers but fails to restore the egg chamber rotation. Expression of kugΔICR.T:Avic\GFP-EGFP in kug58D mutants partially rescues the oviposition defect. It also partially rescues the disrupted subcellular localization of actin, microtubule and collagen IV fibers in the extracellular matrix of the follicle epithelium and fully restores normal oscillating F-actin contractions in kug58D mutant egg chambers.
Expression of kugΔTMICR.T:Avic\GFP-EGFP in kug58D mutants fails to restore normal elongation and rotation of egg chambers or normal oviposition. It also fails to rescue the disrupted subcellular localization of actin, microtubule and collagen IV fibers in the extracellular matrix of the follicle epithelium.
