P{EP} insertion within the first exon, 1bp after the ATG codon.
lethal | maternal effect (with Df(3L)st-j7)
Meiotic (prophase I) mutant spermatocytes contain extra centriole-like structures compared to wild type. The majority of these extra centrioles are present as V shaped pairs, while a few are present as single centriole barrels.
Mutant primary spermatocyte cysts consistently contain 16 cells (as occurs in wild type), but the number of centrioles per cyst is almost always greater than the wild-type number of 64.
Mutant onion stage spermatids often have extra centrioles and have abnormal numbers of nuclei that are often unequal in size.
Most axonemes in the mutant testis have a normal 9+2 structure, although a small proportion have structural defects.
The female meiosis II spindle almost always lacks astral microtubules in mutants.
Mutant larval brains do not show a dramatic decrease in the number of centrioles in mitotic cells compared to wild-type controls.
The mitotic index in mutant third larval instar brains is similar to that of wild type. Mutant larval neuroblasts nucleate robust astral microtubule arrays that participate in spindle formation in a manner indistinguishable from those in wild-type cells. The mutant neuroblasts invariably divide asymmetrically, as occurs in wild type.
Embryos derived from homozygous females mated to wild-type males are generally fertilised (more than 95% of 0-4 hour embryos contain a sperm tail), but are arrested very early in development. The first mitotic spindle is never seen in these embryos. In many cases, pronuclear migration has failed, with male and female pronuclei remaining spatially separated with in the embryo. Female meiosis proceeds normally in these embryos, producing a female pronucleus that is positioned appropriately for subsequent capture by the sperm aster. The sperm centriole in these embryos is rarely associated with microtubules, in contrast to in wild-type embryos. The single sperm-derived centriole is capable of undergoing the first duplication in the mutant embryos.
spd-2G20143 is rescued by spd-2Ubi.GFP