FlyBase Allele Report: Dmel\mts[299]

General Information

Symbol

Dmel\mts²⁹⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0230421

Feature type

allele

Associated gene

Dmel\mts

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Wang et al., 2009)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: D197N.

(Wang et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:7,830,948..7,830,948 [+]

Nucleotide change:

G7830948A

Amino acid change:

D197N | mts-PA; D197N | mts-PB; D197N | mts-PC

Reported amino acid change:

D197N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wang et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval brain

(Wang et al., 2009)

embryonic/larval brain (with mts^s5286)

(Wang et al., 2009)

neuroblast | increased number

(Wang et al., 2009)

neuroblast | increased number (with mts^s5286)

(Wang et al., 2009)

neuroblast | increased number (with mts^XE-2258)

(Wang et al., 2009)

type II neuroblast | somatic clone | increased number

(Ouyang et al., 2011)

Detailed Description

Statement

Reference

mts²⁹⁹ mutant type II neuroblast clones contain multiple neuroblasts.

(Ouyang et al., 2011)

Mutant larvae have highly enlarged brain lobes and produce supernumerary neuroblasts in the brain.

mts²⁹⁹/mts^s5286 central brains contain around 1000 neuroblasts at 96 hours after larval hatching, compared to around 100 neuroblasts in wild-type central brains at this time. An increase in BrdU incorporation is also observed in the mutants.

The mitotic spindle is misaligned with the cortical crescent in 44% of neuroblasts cells in the mts²⁹⁹/mts^unspecified mutant.

(Wang et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mts²⁹⁹ has abnormal neuroanatomy | somatic clone phenotype, suppressible by Scer\GAL4^insc-Mz1407/numb^TS4A.UAS

(Ouyang et al., 2011)

mts²⁹⁹/mts^s5286 has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP

(Wang et al., 2009)

mts²⁹⁹/mts^s5286 has increased cell number phenotype, suppressible by Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP

(Wang et al., 2009)

mts²⁹⁹ has abnormal neuroanatomy phenotype, suppressible by polo^GFP

(Wang et al., 2009)

mts²⁹⁹ has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^UAS.Venus

(Wang et al., 2009)

NOT suppressed by

Statement

Reference

mts²⁹⁹ has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/numb^UAS.cOa

(Ouyang et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

mts²⁹⁹ has type II neuroblast | increased number | somatic clone phenotype, suppressible by Scer\GAL4^insc-Mz1407/numb^TS4A.UAS

(Ouyang et al., 2011)

mts²⁹⁹ has neuroblast | increased number phenotype, suppressible by polo^GFP

(Wang et al., 2009)

mts²⁹⁹ has neuroblast | increased number phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^UAS.Venus

(Wang et al., 2009)

mts²⁹⁹/mts^s5286 has neuroblast | increased number phenotype, suppressible by Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP

(Wang et al., 2009)

mts²⁹⁹/mts^s5286 has embryonic/larval brain phenotype, suppressible by Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP

(Wang et al., 2009)

NOT suppressed by

Statement

Reference

mts²⁹⁹ has type II neuroblast | increased number | somatic clone phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/numb^UAS.cOa

(Ouyang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of numb^{TS4A.Scer\UAS} completely suppresses the ectopic type II neuroblasts found in mts²⁹⁹ type II neuroblast clones.

Expression of numb^Scer\UAS.cOa does not suppress the ectopic type II neuroblasts found in mts²⁹⁹ type II neuroblast clones.

(Ouyang et al., 2011)

Expression of numb^{PTB.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4¹⁴⁰⁷ significantly suppresses the neuroblast overgrowth phenotype seen in mts²⁹⁹/mts^s5286 larvae.

polo^T:Avic\GFP can significantly suppress the neuroblast overgrowth phenotype seen in mts²⁹⁹ mutants.

Expression of polo^{Scer\UAS.T:Avic\GFP-YFP.Venus} under the control of Scer\GAL4¹⁴⁰⁷ can significantly suppress the neuroblast overgrowth phenotype seen in mts²⁹⁹ mutants.

(Wang et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mts²⁹⁹ is rescued by Scer\GAL4^insc-Mz1407/mts^UAS.cHa

(Wang et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mts²⁹⁹

(Rui et al., 2020, Gil-Ranedo et al., 2019, Ouyang et al., 2011, Wang et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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