FlyBase Allele Report: Dmel\mts[s5286]

General Information

Symbol

Dmel\mts^s5286

Species

D. melanogaster

Name

FlyBase ID

FBal0009325

Feature type

allele

Associated gene

Dmel\mts

Associated Insertion(s)

P{lacW}mts^s5286

Carried in Construct

Also Known As

l(2)s5286

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{lacW}mts^s5286

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage (with mts²⁹⁹)

(Wang et al., 2009)

increased cell number (with mts²⁹⁹)

(Wang et al., 2009)

increased occurrence of cell division (with mts²⁹⁹)

(Wang et al., 2009)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

embryonic/larval brain (with mts²⁹⁹)

(Wang et al., 2009)

neuroblast | increased number (with mts²⁹⁹)

(Wang et al., 2009)

Detailed Description

Statement

Reference

mts²⁹⁹/mts^s5286 central brains contain around 1000 neuroblasts at 96 hours after larval hatching, compared to around 100 neuroblasts in wild-type central brains at this time. An increase in BrdU incorporation is also observed in the mutants.

(Wang et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mts²⁹⁹/mts^s5286 has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP

(Wang et al., 2009)

mts²⁹⁹/mts^s5286 has increased cell number phenotype, suppressible by Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP

(Wang et al., 2009)

NOT Enhancer of

Statement

Reference

mts^s5286 is a non-enhancer of visible phenotype of Scer\GAL4^ptc-559.1, smo^RNAi.UAS.WIZ

(Casso et al., 2008)

Suppressor of

Statement

Reference

mts^s5286 is a suppressor | partially of abnormal mitotic cell cycle | maternal effect phenotype of png¹⁰⁵⁸/png³³¹⁸

(Lee et al., 2001)

mts^s5286 is a suppressor of abnormal mitotic cell cycle | maternal effect phenotype of png¹⁰⁵⁸

(Lee et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

mts²⁹⁹/mts^s5286 has neuroblast | increased number phenotype, suppressible by Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP

(Wang et al., 2009)

mts²⁹⁹/mts^s5286 has embryonic/larval brain phenotype, suppressible by Scer\GAL4^insc-Mz1407/numb^PTB.UAS.GFP

(Wang et al., 2009)

NOT Enhancer of

Statement

Reference

mts^s5286 is a non-enhancer of wing vein phenotype of Scer\GAL4^ptc-559.1, smo^RNAi.UAS.WIZ

(Casso et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of numb^{PTB.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4¹⁴⁰⁷ significantly suppresses the neuroblast overgrowth phenotype seen in mts²⁹⁹/mts^s5286 larvae.

(Wang et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mts⁰²⁴⁹⁶

(BDGP Project Members, 1994-1999)

mts^k06009

(BDGP Project Members, 1994-1999)

mts^k16501

(BDGP Project Members, 1994-1999)