FlyBase Allele Report: Dmel\asun[f02815]

General Information

Symbol

Dmel\asun^f02815

Species

D. melanogaster

Name

FlyBase ID

FBal0222709

Feature type

allele

Associated gene

Dmel\asun

Associated Insertion(s)

PBac{WH}asun^f02815

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Anderson et al., 2009)

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{WH}asun^f02815

Cytology

Description

Insertion in the second intron.

(Anderson et al., 2009)

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:16,266,328..16,266,328 [+]

(Sitaram et al., 2012, Anderson et al., 2009, FlyBase Curators, 2008-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Sitaram et al., 2012)

male sterile

(Jodoin et al., 2013, Anderson et al., 2009)

male sterile (with Df(3R)Exel7329)

(Anderson et al., 2009)

viable

(Anderson et al., 2009)

Phenotype Manifest In

aster & primary spermatocyte in meiotic prophase I

(Anderson et al., 2009)

centrosome

(Sitaram et al., 2012)

investment cone

(Anderson et al., 2009)

microtubule basal body & elongation stage spermatid

(Anderson et al., 2009)

nucleus & elongation stage spermatid

(Anderson et al., 2009)

onion stage spermatid

(Anderson et al., 2009)

onion stage spermatid (with Df(3R)Exel7329)

(Anderson et al., 2009)

primary spermatocyte

(Anderson et al., 2009)

primary spermatocyte (with Df(3R)Exel7329)

(Anderson et al., 2009)

spermatid (with asun^d93), with bor^+t4

(Sitaram et al., 2012)

spermatocyte

(Sitaram et al., 2012)

spermatozoon

(Anderson et al., 2009)

spindle & spermatocyte

(Anderson et al., 2009)

Detailed Description

Statement

Reference

The testes of asun^f02815 mutants contain an increased fraction of prophase I spermatocytes compared to controls. The microtubules that surround the nucleus of wild type prophase spermatocytes are absent in asun^f02815 mutants.

Cortical centrosomes are rarely seen in asun^f02815 mutant prophase spermatocytes.

asun^d93/asun^f02815 mutant spermatids (expressing bor^+t4 to restore bor[+] function) display frequent nucleus-nebenkern and nucleus-basal body uncoupling. Nebenkern-basal body attachments are unaffected.

(Sitaram et al., 2012)

Homozygous adults are viable and appear normal.

Mutant females have only slightly decreased fertility.

Homozygous males are almost completely sterile (8% fertility). The testes of mutant males are similar in overall size and shape to those of control males, but their seminal vesicles are largely empty. Mature sperm are formed in small amounts in the mutant testes and are either immotile or weakly motile.

Actin arrays seem grossly normal in homozygous spermatocytes and astral microtubule arrays are similar in size to those of wild type. Mutant spermatocytes show normal cortical positioning of the centrosomes in G2 and release of the centrosomes upon M phase entry, however, subsequent nucleus-centrosome coupling is defective, with a striking loss of association between asters and the nucleus during prophase in the primary spermatocytes. The number of asters in mutant prophase spermatocytes varies from two to four and the majority of mutant prophase I spermatocytes contain unattached, divided asters. The fraction of homozygous and Mat89Bb^f02815/Df(3R)Exel7329 spermatocytes that are in prophase is increased compared to wild type.

The majority of mutant spermatocytes that progress through meiotic divisions show gross defects in spindle assembly, e.g. tetrapolar spindles, and chromosome segregation.

Most mutant cysts have reduced number of onion-stage spermatids (sometimes as few as 16), with a variable number of irregularly sized nuclei and one large Nebenkern. Only 33% of homozygous onion-stage spermatids have the normal 1:1 Nebenkern to nuclei ratio. 47% of homozygous onion-stage spermatids have variably sized nuclei.

Only 9% of Mat89Bb^f02815/Df(3R)Exel7329 onion-stage spermatids have the normal 1:1 Nebenkern to nuclei ratio. 56% of homozygous onion-stage spermatids have variably sized nuclei.

Mutant spermatids that have failed to undergo cytokinesis show impaired nucleus-basal body coupling. However, axonemes do nucleate from the free basal bodies of the mutant spermatids. The nuclei and basal bodies of mutant spermatids in elongating bundles are often randomly distributed throughout the length of the bundle, and nuclei remain round. Actin cones seem to move down the axoneme independent of one another and are typically dispersed along the entire bundle length in the mutant spermatids.

(Anderson et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Lis-1[+]/Lis-1^k11702, asun^f02815 has abnormal meiotic cell cycle phenotype

(Sitaram et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

asun^f02815 has spermatid phenotype, enhanceable by Df(3L)fz-GF3b/+

(Anderson et al., 2009)

asun^f02815 has spermatid phenotype, enhanceable by Dhc64C[+]/Dhc64C^4-19

(Anderson et al., 2009)

asun^f02815 has spermatid phenotype, enhanceable by Dhc64C[+]/Dhc64C^6-10

(Anderson et al., 2009)

Other

Statement

Reference

Lis-1[+]/Lis-1^k11702, asun^f02815 has testis phenotype

(Sitaram et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The testes of Lis-1^k11702/+ ; asun^f02815 mutant males are small compared to controls. There is an extreme paucity of sperm bundles and most of the cells are late G2 primary spermatocytes, indicative of a severe G2 block.

The testes of Lis-1^k11702 ; asun^f02815/+ males are comparable in size to controls.

(Sitaram et al., 2012)

A single copy of Dhc64C^4-19, Dhc64C^6-10 or Df(3L)fz-GF3b introduced into the Mat89Bb^f02815 background significantly increases the incidence of multi-nucleated spermatids (from 64% to 93, 84 and 88%, respectively).

(Anderson et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

asun^f02815 is rescued by asun^{βTub85D.mCherry}

(Jodoin et al., 2013)

asun^f02815 is rescued by asun^{βTub85D.Tag:NLS(SV40-largeT),mCherry}

(Jodoin et al., 2013)

asun^f02815 is rescued by Scer\GAL4^{VP16.nanos.UTR}/asun^UASp.Tag:MYC

(Anderson et al., 2009)