βTub85D promoter sequences drive expression of asun containing a mutation in the candidate nuclear localization signal (LKASKRR to LKASAAA). The protein is tagged at the N-terminal end with mCherry.
asunmutNLS.βTub85D.mCherry partially rescues asunf02815
asunmutNLS.βTub85D.T:Disc\RFP-mCherry partially rescues the sterility of asunf02815 males.