FlyBase Allele Report: Dmel\cher[JF02077]

General Information

Symbol

Dmel\cher^JF02077

Species

D. melanogaster

Name

FlyBase ID

FBal0220568

Feature type

allele

Associated gene

Dmel\cher

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02077}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.3.31)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.3.31)

Mutagen

in vitro construct

(Perkins et al., 2009.3.31)

Progenitor genotype

Carried in construct

P{TRiP.JF02077}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.3.31)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02077

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of myopathy

CEA

(González-Morales et al., 2017)

CEA with cher^KK107518

(González-Morales et al., 2017)

model of distal myopathy 4

CEA

(Ader et al., 2022)

model of myofibrillar myopathy 5

CEA

(Ader et al., 2022)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of myopathy

is exacerbated by sls¹

(González-Morales et al., 2017)

is exacerbated by sls^j1D7

(González-Morales et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult cardial cell, with Scer\GAL4^{Hand.ΔVM.Switch}, cher^KK107518

(Ader et al., 2022)

adult heart, with Scer\GAL4^{Hand.ΔVM.Switch}

(Ader et al., 2022)

adult heart, with Scer\GAL4^{Hand.ΔVM.Switch}, cher^KK107518

(Ader et al., 2022)

adult heart chamber 1 | adult stage, with Scer\GAL4^{Hand.ΔVM.Switch}, cher^KK107518

(Ader et al., 2022)

axon | embryonic stage, with Scer\GAL4^elav.PLu

(Jeong et al., 2017)

H zone | adult stage, with Scer\GAL4^Mef2.PU

(González-Morales et al., 2017)

indirect flight muscle cell | adult stage, with Scer\GAL4^Mef2.PU

(González-Morales et al., 2017)

indirect flight muscle cell | adult stage, with Scer\GAL4^Mef2.PU, cher^KK107518

(González-Morales et al., 2017)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage, with Scer\GAL4^elav.PLu

(Jeong et al., 2017)

myofibril | adult stage, with Scer\GAL4^Mef2.PU

(González-Morales et al., 2017)

sarcomere | adult stage, with Scer\GAL4^{Hand.ΔVM.Switch}, cher^KK107518

(Ader et al., 2022)

sarcomere | adult stage, with Scer\GAL4^Mef2.PU

(González-Morales et al., 2017)

sarcomere | adult stage, with Scer\GAL4^Mef2.PU, cher^KK107518

(González-Morales et al., 2017)

Z disc | adult stage, with Scer\GAL4^Mef2.PU

(González-Morales et al., 2017)

Detailed Description

Statement

Reference

The expression of cher^JF02077 under the control of Scer\GAL4^Mef2.PU leads to significant decrease in adult viability and to a flightless phenotype, associated with defects in adult indirect flight muscles, namely shredding of myofibrils, severe decrease in the number of recognizable sarcomeres, widened or smaller/fractured Z-disc and actin incorporation into the H-zone, as compared to controls; the severe defects in sarcomere structure are enhanced by the additional expression of cher^KK107518.

(González-Morales et al., 2017)

Expression of cher^JF02077 under the control of Scer\GAL4^elav.PLu in embryos leads to increased frequency of defasciculation defects in intersegmental nerve b motor axons compared to controls.

(Jeong et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Mef2.PU, cher^JF02077 has partially lethal - majority live phenotype, enhanceable by sls¹/sls[+]

(González-Morales et al., 2017)

Scer\GAL4^Mef2.PU, cher^JF02077 has partially lethal - majority live phenotype, enhanceable by sls[+]/sls^j1D7

(González-Morales et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Mef2.PU, cher^JF02077 has myofibril | adult stage phenotype, enhanceable by sls¹/sls[+]

(González-Morales et al., 2017)

Scer\GAL4^Mef2.PU, cher^JF02077 has sarcomere | adult stage phenotype, enhanceable by sls¹/sls[+]

(González-Morales et al., 2017)

Scer\GAL4^Mef2.PU, cher^JF02077 has myofibril | adult stage phenotype, enhanceable by sls[+]/sls^j1D7

(González-Morales et al., 2017)

Scer\GAL4^Mef2.PU, cher^JF02077 has sarcomere | adult stage phenotype, enhanceable by sls[+]/sls^j1D7

(González-Morales et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The semi-lethality and the myofibril shredding defects resulting from the expression of cher^JF02077 under the control of Scer\GAL4^Mef2.PU is enhanced by heterozygosity for either sls¹ or sls^j1D7, even leading to the loss of the sarcomere structure.

(González-Morales et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26307

y¹ v¹; P{TRiP.JF02077}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

cher^JF02077

(González-Morales et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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