FlyBase Allele Report: Dmel\Rac2[UAS.cARa]

General Information

Symbol

Dmel\Rac2^UAS.cARa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Avet-Rochex

FlyBase ID

FBal0219750

Feature type

allele

Associated gene

Dmel\Rac2

Associated Insertion(s)

Carried in Construct

P{UAS-Rac2.AR}

Also Known As

UAS-Rac2

Key Links

Transgenic product class

wild_type

(Avet-Rochex et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Avet-Rochex et al., 2007)

Mutagen

in vitro construct

(Avet-Rochex et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Rac2.AR}

Cytology

Description

UASt regulatory sequences drive expression of Rac2.

(Avet-Rochex et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rac2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^da.G32

(Avet-Rochex et al., 2007)

abnormal immune response, with Scer\GAL4^srp.D.cCa

(Avet-Rochex et al., 2007)

lethal, with Scer\GAL4^srp.D.cCa

(Avet-Rochex et al., 2007)

visible, with Scer\GAL4^en-e16E

(Avet-Rochex et al., 2007)

visible, with Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2007)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2007)

wing | posterior, with Scer\GAL4^en-e16E

(Avet-Rochex et al., 2007)

Detailed Description

Statement

Reference

Most animals expressing Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^srp.D.cCa do not hatch at 20 or 25[o]C. Adult expressing Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^srp.D.cCa which are raised at 17[o]C and then transferred to 25[o]C show increased sensitivity to P.aeruginosa following septic or oral infections compared to control flies.

Animals expressing Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^da.G32 show increased sensitivity to P.aeruginosa following oral infection compared to control flies.

Flies expressing Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^GMR.PF have reduced, glossy, rough eyes.

The most posterior part of the wing is mis-shaped in flies expressing Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^en-e16E.

(Avet-Rochex et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rac2^UAS.cARa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Paer\ExoS^GAP.UAS, Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2007)

Rac2^UAS.cARa, Scer\GAL4^da.G32 has abnormal immune response phenotype, suppressible by Paer\ExoS^GAP.UAS, Scer\GAL4^da.G32

(Avet-Rochex et al., 2007)

Rac2^UAS.cARa, Scer\GAL4^en-e16E has visible phenotype, suppressible by Paer\ExoS^GAP.UAS, Scer\GAL4^en-e16E

(Avet-Rochex et al., 2007)

Suppressor of

Statement

Reference

Rac2^UAS.cARa/Scer\GAL4^He.PZ is a suppressor of abnormal immune response | recessive | larval stage phenotype of Zir^BG00267

(Sampson et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rac2^UAS.cARa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Paer\ExoS^GAP.UAS, Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2007)

Rac2^UAS.cARa, Scer\GAL4^en-e16E has wing | posterior phenotype, suppressible by Paer\ExoS^GAP.UAS, Scer\GAL4^en-e16E

(Avet-Rochex et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^He.PZ rescues the reduced rate of encapsulation of eggs of the avirulent wasp strain L. boulardi G486 that is seen in Zir^BG00267 larvae.

Expression of either Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^He.PZ rescues the reduced phagocytosis index against E. coli and S. aureus seen in isolated plasmatocytes from homozygous Zir^BG00267 larvae.

(Sampson et al., 2012)

Xenogenetic Interactions

Statement

Reference

Co-expression of Zzzz\ExoS^GAP.Scer\UAS suppresses the rough eye phenotype caused by expression of Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^GMR.PF.

Co-expression of Zzzz\ExoS^GAP.Scer\UAS suppresses the wing phenotype caused by expression of Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^en-e16E.

The increased sensitivity to P.aeruginosa following oral infection that is seen in flies expressing Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^da.G32 is suppressed by co-expression of Zzzz\ExoS^GAP.Scer\UAS.

(Avet-Rochex et al., 2007)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

26650

P{UAS-Rac2.AR}1-2, w¹¹¹⁸

26652

w¹¹¹⁸; P{UAS-Rac2.AR}3-2/TM3, Sb¹