Imprecise excision of the P{EPgy2}Atg7EY10058 insertion, resulting in a deletion that removes portions of both the Atg7 and sec6 genes.
Compared with wild-type, Atg7d14/Atg7d77 flies show reduce memory scores at a young age (3 days of age), which further declines relative to controls at later time points (20 days of age). Wild-type flies show a decline in both anesthesia-sensitive and olfactory short-term memory, both of which can be ameliorated by spermidine-feeding. Notably, the olfactory short term memory-protective effects of spermidine-administration are eliminated in the mutants.
Nutrient-deprived Atg7d77/Atg7d14 mutant flies display reduced levels of autophagy (punctate Lysotracker staining) in region two of the germarium and in stage eight degenerating egg chambers compared to controls.
Fewer apoptotic (TUNEL positive) cells are seen in region two cysts in nutrient-deprived Atg7d77/Atg7d14 mutant flies than are seen in wild type. Degenerating stage eight egg chambers in starved Atg7d77/Atg7d14 mutants show low or no TUNEL-positive staining compared with controls, although nuclear DNA condensation is still observed in these egg chambers.
Atg7d14/Atg7d77 fat body cells show a strong reduction of autophagosomes and autolysosomes following 3 hours of starvation compared to control cells.
Midgut cells of Atg7d14/Atg7d77 larvae show a strong reduction of autophagosomes and autolysosomes compared to control larvae, at the wandering stage, just prior to pupariation. No major defects are seen in elimination of the larval midgut or in adult midgut epithelium formation in Atg7d14/Atg7d77 animals during metamorphosis. However, DNA fragmentation indicative of cell death is greatly reduced in the mutant midgut at the time of pupariation (DNA fragmentation is readily detected at this stage in control midguts) and there is an approximately 4 hour increase in the average time required to complete metamorphosis in the mutant animals.
Under conditions of complete starvation or a sugar-only diet, Atg7d14/Atg7d77 mutants show an accelerated lethality compared to control flies. The mutant flies also show a reduced life span compared to controls under normal environmental conditions.
Atg7d14/Atg7d77 mutants are hypersensitive to treatment with paraquat compared to controls.
Atg7d14/Atg7d77 flies show a significant age-dependent decline in climbing performance. The brains of mutant adults contain ubiquitin-positive inclusion bodies in the neurons, with these structures being seen in 3 day old adults, and increasing in number with age. The brains of 30 day old mutant flies contain dead neurons in addition to the inclusion bodies, show moderate vacuolization and most brain cells show extensive DNA fragmentation (assayed using TUNEL labeling).
Atg7d14/Atg7d77, Pink1B9 has visible | adult stage phenotype, suppressible by Hsap\DNM1LUAS.Tag:MYC,Tag:polyHis/Scer\GAL4Mhc.PU
Atg7d14/Atg7d77, Pink1B9 has visible | adult stage phenotype, suppressible by Hsap\DNM1LS616D.UAS.Tag:MYC,Tag:polyHis/Scer\GAL4Mhc.PU
Atg7d14/Atg7d77, Pink1B9 has visible | adult stage phenotype, suppressible | partially by Hsap\DNM1LS616A.UAS.Tag:MYC,Tag:polyHis/Scer\GAL4Mhc.PU
Atg7d14/Atg7d77 is a non-suppressor of visible | adult stage phenotype of Pink1B9
Atg7d14/Atg7d77, Pink1B9 has mitochondrion | adult stage phenotype, suppressible | partially by Hsap\DNM1LS616D.UAS.Tag:MYC,Tag:polyHis/Scer\GAL4Mhc.PU
Atg7d14/Atg7d77, Pink1B9 has adult thorax phenotype, suppressible by Hsap\DNM1LUAS.Tag:MYC,Tag:polyHis/Scer\GAL4Mhc.PU
Atg7d14/Atg7d77, Pink1B9 has adult thorax phenotype, suppressible | partially by Hsap\DNM1LS616D.UAS.Tag:MYC,Tag:polyHis/Scer\GAL4Mhc.PU
Atg7d14/Atg7d77, Pink1B9 has adult thorax phenotype, suppressible | partially by Hsap\DNM1LS616A.UAS.Tag:MYC,Tag:polyHis/Scer\GAL4Mhc.PU
Atg7d14/Atg7d77, Pink1B9 has mitochondrion | adult stage phenotype, suppressible by Hsap\DNM1LUAS.Tag:MYC,Tag:polyHis/Scer\GAL4Mhc.PU
Atg7d14/Atg7d77, Pink1B9 has adult thorax phenotype, non-suppressible | partially by Hsap\DNM1LS616A.UAS.Tag:MYC,Tag:polyHis/Scer\GAL4Mhc.PU
Atg7d14/Atg7d77, Pink1B9 has mitochondrion | adult stage phenotype, non-suppressible by Hsap\DNM1LS616A.UAS.Tag:MYC,Tag:polyHis/Scer\GAL4Mhc.PU
Atg7d14/Atg7d77 is a non-enhancer of mitochondrial crista | adult stage phenotype of Pink1B9
Atg7d14/Atg7d77 is a non-enhancer of indirect flight muscle cell phenotype of Pink1B9
Atg7d14/Atg7d77 is a non-enhancer of mitochondrion | adult stage phenotype of Pink1B9
Atg7d14/Atg7d77 is a non-suppressor of mitochondrial crista | adult stage phenotype of Pink1B9
Atg7d14/Atg7d77 is a non-suppressor of indirect flight muscle cell phenotype of Pink1B9
Atg7d14/Atg7d77 is a non-suppressor of mitochondrion | adult stage phenotype of Pink1B9