FlyBase Allele Report: Dmel\Pabp2[55]

General Information

Symbol

Dmel\Pabp2⁵⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0219382

Feature type

allele

Associated gene

Dmel\Pabp2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Benoit et al., 2005)

Mutagen

P-element activity

(Benoit et al., 2005)

Progenitor genotype

Pabp2^EP2264

(Benoit et al., 2005)

Cytology

Description

Imprecise excision of P{EP}Pabp2^EP2264 has deleted the Pabp2 coding region - from 104bp upstream of the start codon to 56bp downstream of the stop codon. Partial P{EP} sequence remains.

(Benoit et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2R:8,131,930..8,133,235 [+]

Comment:

Deletion resulting from partial excision of P{EP}Pabp2^EP2264. Part of the P{EP} sequence remains.

(Benoit et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.YFP.cEa

(Chou et al., 2015)

modeled by Hsap\TARDBP^{A315T.UAS.YFP}

(Chou et al., 2015)

modeled by Hsap\TARDBP^{D169G.UAS.YFP}

(Chou et al., 2015)

modeled by Hsap\TARDBP^{G298S.UAS.YFP}

(Chou et al., 2015)

ameliorates oculopharyngeal muscular dystrophy

modeled by Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

exacerbates autosomal recessive intellectual developmental disorder

modeled by Nab2^ex3

(Pak et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage

(Chou et al., 2015)

lethal (with Df(2R)Exel6056)

(Kahsai et al., 2016)

lethal | first instar larval stage | recessive

(Benoit et al., 2005)

partially lethal - majority die | late embryonic stage | recessive

(Benoit et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Pabp2⁵⁵ does not cause a neurodegeneration phenotype (no visible depigmentation) in the eye. Pabp2⁵⁵ larvae show significant locomotor dysfunction (significant increase in the amount of time taken for the larva to turn from dorsal side and resume crawling on the ventral side) compared to controls.

(Chou et al., 2015)

Most Pabp2⁵⁵ homozygotes dies as late embryos. mRNA extracted from the few that survive to first instar larval stage has a different distribution of polyA tail lengths compared to wild-type: the peak seen in the 70-100 nucleotide range in wild-type is shifted to below 70 in the mutant.

Pabp2⁵⁵ germ-line clones can not be recovered.

(Benoit et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Pabp2[+]/Pabp2⁵⁵ is an enhancer of visible | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

Pabp2[+]/Pabp2⁵⁵ is an enhancer of decreased size | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

Pabp2⁵⁵ is an enhancer of visible phenotype of Hsap\TARDBP^{A315T.UAS.YFP}, Scer\GAL4^GMR.PU

(Chou et al., 2015)

Pabp2⁵⁵ is an enhancer of abnormal neuroanatomy | adult stage phenotype of Hsap\TARDBP^{A315T.UAS.YFP}, Scer\GAL4^GMR.PU

(Chou et al., 2015)

Pabp2⁵⁵ is an enhancer of visible phenotype of Hsap\TARDBP^{D169G.UAS.YFP}, Scer\GAL4^GMR.PU

(Chou et al., 2015)

Pabp2⁵⁵ is an enhancer of abnormal neuroanatomy | adult stage phenotype of Hsap\TARDBP^{D169G.UAS.YFP}, Scer\GAL4^GMR.PU

(Chou et al., 2015)

Pabp2⁵⁵ is an enhancer of abnormal locomotor behavior | larval stage phenotype of Hsap\TARDBP^{D169G.UAS.YFP}, Scer\GAL4^Toll-6-D42

(Chou et al., 2015)

Pabp2⁵⁵ is an enhancer of abnormal locomotor behavior | larval stage phenotype of Hsap\TARDBP^{G298S.UAS.YFP}, Scer\GAL4^Toll-6-D42

(Chou et al., 2015)

Pabp2⁵⁵ is an enhancer of visible phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^GMR.PU

(Chou et al., 2015)

Pabp2⁵⁵ is an enhancer of abnormal neuroanatomy | adult stage phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^GMR.PU

(Chou et al., 2015)

Pabp2⁵⁵ is an enhancer of abnormal locomotor behavior | larval stage phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^Toll-6-D42

(Chou et al., 2015)

Pabp2[+]/Pabp2⁵⁵ is an enhancer of visible phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Pak et al., 2011)

Suppressor of

Statement

Reference

Pabp2[+]/Pabp2⁵⁵ is a suppressor of visible phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

Pabp2[+]/Pabp2⁵⁵ is a suppressor of partially lethal - majority die | maternal effect | embryonic stage phenotype of orb^mel

(Benoit et al., 2005)

Other

Statement

Reference

Nab2^EP3716, Pabp2⁵⁵, Scer\GAL4^GMR.PU has partially lethal phenotype

(Lee et al., 2020)

Pabp2[+]/Pabp2⁵⁵, twin^GS8115 has partially lethal - majority die | recessive | maternal effect | embryonic stage phenotype

(Benoit et al., 2005)

Phenotype Manifest In

Enhancer of

Statement

Reference

Pabp2[+]/Pabp2⁵⁵ is an enhancer of eye phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020, Pak et al., 2011)

Pabp2⁵⁵ is an enhancer of eye phenotype of Hsap\TARDBP^{D169G.UAS.YFP}, Scer\GAL4^GMR.PU

(Chou et al., 2015)

Pabp2⁵⁵ is an enhancer of eye phenotype of Hsap\TARDBP^UAS.YFP.cEa, Scer\GAL4^GMR.PU

(Chou et al., 2015)

Pabp2⁵⁵ is an enhancer of eye phenotype of Hsap\TARDBP^{A315T.UAS.YFP}, Scer\GAL4^GMR.PU

(Chou et al., 2015)

Suppressor of

Statement

Reference

Pabp2[+]/Pabp2⁵⁵ is a suppressor of wing phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Pabp2⁵⁵/+ strongly enhances the mutant eye phenotype caused by expression of Nab2^EP3716 under the control of Scer\GAL4^GMR.PU, resulting in a smaller, more disorganised and blackened eye.

(Pak et al., 2011)

Xenogenetic Interactions

Statement

Reference

Pabp2⁵⁵ suppresses the wing posture defects seen upon expression of Hsap\PABPN1^17ala.Act88F.

(Chartier et al., 2015)

Pabp2⁵⁵ enhances the neurodegeneration phenotype (visible as depigmentation of the eye) in flies with expression of Hsap\TARDBP^{Scer\UAS.T:Avic\GFP-YFP.cEa}, Hsap\TARDBP^{A315T.Scer\UAS.T:Avic\GFP-YFP} or Hsap\TARDBP^{D169G.Scer\UAS.T:Avic\GFP-YFP} driven by Scer\GAL4^GMR.PU.

Pabp2⁵⁵ significantly enhances locomotor dysfunction seen in larvae with expression of Hsap\TARDBP^{Scer\UAS.T:Avic\GFP-YFP.cEa}, Hsap\TARDBP^{G298S.Scer\UAS.T:Avic\GFP-YFP} or Hsap\TARDBP^{D169G.Scer\UAS.T:Avic\GFP-YFP} driven by Scer\GAL4^GMR.PU.

(Chou et al., 2015)

Complementation and Rescue Data

Rescued by

Pabp2⁵⁵/Df(2R)CA53 is rescued by Pabp2^+t.g

(Benoit et al., 2005)

Not rescued by

Pabp2⁵⁵/Df(2R)CA53 is not rescued by Pabp2^I61S.g

(Benoit et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Pabp2⁵⁵

(Lee et al., 2020, Sarov et al., 2016, Chartier et al., 2015, Chou et al., 2015, Pak et al., 2011, Benoit et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (8)

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