FlyBase Allele Report: Dmel\NimC4[KO]

General Information

Symbol

Dmel\NimC4^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0218199

Feature type

allele

Associated gene

Dmel\NimC4

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Kurant et al., 2008)

I-SceI

(Kurant et al., 2008)

FLPase

(Kurant et al., 2008)

I-CreI

(Kurant et al., 2008)

gene targeting by homologous recombination

(Kurant et al., 2008)

Progenitor genotype

Cytology

Description

Both in-frame ATG codons of the NimC4 open reading frame have been replaced by stop codons.

(Kurant et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Kurant et al., 2008)

Phenotype Manifest In

embryonic/larval glial cell

(Kurant et al., 2008)

embryonic/larval plasmatocyte

(Kurant et al., 2008)

Detailed Description

Statement

Reference

NimC4^KO mutant pupae exhibit do not exhibit defects in phagocytic clearance of ddaC neuron dendrite debris following pupal stage dendritic pruning. As in controls, debris from injured larval dendrites (severed with an infrared laser) is completely cleared.

(Han et al., 2014)

The CNS of NimC4^KO mutant stage 16 embryos exhibits defects in the engulfment of apoptotic neurons. There is an increase in the volume of unengulfed apoptotic particles compared to controls.

(Shklyar et al., 2013)

Homozygotes have no gross morphological abnormalities.

The number, position and overall morphology of central nervous system glia (including the highly phagocygotic cell body glia) appear normal in homozygous embryos. However, the clearance of apoptotic cells in these embryos is markedly impaired. The number and total volume of apoptotic particles in the central nervous system (CNS) is increased about 2-fold compared to wild type, and the apoptotic particles are much less likely to be engulfed, with the fraction of particles that are completely untouched being increased 3-fold compared to wild type. The number, migration pattern and morphology of the macrophages appear normal. However, the number and volume of apoptotic particles outside of the CNS is strongly increased compared to wild type and many particles remain unengulfed, often forming large clusters near the macrophages.

Search behaviour and motility of phagocytes are normal in homozygous embryos, but many apoptotic particles remain completely untouched for extended periods of time or are briefly touched without being tethered. Many apoptotic particles attach to the mutant phagocyte cell surface without being engulfed Occasionally, incomplete phagocytic cups are seen.

(Kurant et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

NimC4^KO/NimC4^KO is an enhancer of increased cell death | embryonic stage phenotype of Scer\GAL4^elav.PU, hid^UAS.cUa

(Shklover et al., 2015)

NOT Enhancer of

Statement

Reference

NimC4^KO/NimC4^KO is a non-enhancer of decreased cell number | embryonic stage phenotype of Scer\GAL4^elav.PU, hid^UAS.cUa

(Shklover et al., 2015)

NOT Suppressor of

Statement

Reference

NimC4^KO/NimC4^KO is a non-suppressor of decreased cell number | embryonic stage phenotype of Scer\GAL4^elav.PU, hid^UAS.cUa

(Shklover et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

NimC4^KO/NimC4^KO is an enhancer of larval ventral nerve cord | embryonic stage phenotype of Scer\GAL4^elav.PU, hid^UAS.cUa

(Shklover et al., 2015)

NOT Enhancer of

Statement

Reference

NimC4^KO/NimC4^KO is a non-enhancer of neuron | embryonic stage phenotype of Scer\GAL4^elav.PU, hid^UAS.cUa

(Shklover et al., 2015)

NOT Suppressor of

Statement

Reference

NimC4^KO/NimC4^KO is a non-suppressor of neuron | embryonic stage phenotype of Scer\GAL4^elav.PU, hid^UAS.cUa

(Shklover et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

NimC4^KO is rescued by Scer\GAL4^repo/NimC4^UAS.GFP

(Shklyar et al., 2013)

NimC4^KO is rescued by Scer\GAL4^repo/NimC4^ΔTM.UAS.GFP

(Shklyar et al., 2013)

NimC4^KO is rescued by NimC4^Tag:MYC

(Kurant et al., 2008)

NimC4^KO is rescued by NimC4^+tKa

(Kurant et al., 2008)

NimC4^KO is rescued by NimC4^ΔTM

(Kurant et al., 2008)

Not rescued by

NimC4^KO is not rescued by Scer\GAL4^repo/NimC4^{ΔEMI-NIM2.UAS.GFP}

(Shklyar et al., 2013)

NimC4^KO is not rescued by NimC4^{ΔEMI.UAS.GFP}/Scer\GAL4^repo

(Shklyar et al., 2013)

NimC4^KO is not rescued by NimC4^{ΔNIM1.UAS.GFP}/Scer\GAL4^repo

(Shklyar et al., 2013)

NimC4^KO is not rescued by Scer\GAL4^repo/NimC4^{ΔNIM2.UAS.GFP}

(Shklyar et al., 2013)

NimC4^KO is not rescued by NimC4^{ΔNIM3-4.UAS.GFP}/Scer\GAL4^repo

(Shklyar et al., 2013)

NimC4^KO is not rescued by Scer\GAL4^repo/NimC4^{ΔNIM3-4ΔTM.UAS.GFP}

(Shklyar et al., 2013)

NimC4^KO is not rescued by NimC4^{ΔNIM3ΔTM.UAS.GFP}/Scer\GAL4^repo

(Shklyar et al., 2013)

NimC4^KO is not rescued by Scer\GAL4^repo/NimC4^{ΔNIM4ΔTM.UAS.GFP}

(Shklyar et al., 2013)

NimC4^KO is not rescued by NimC4^ΔEMI

(Kurant et al., 2008)

Comments

Expression of NimC4^{ΔEMI-NIM2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^repo does not rescue the defects in the engulfment of apoptotic neurons seen in the CNS of NimC4^KO mutant embryos.

Expression of NimC4^{ΔEMI-NIM2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^repo rescues the defects in the engulfment of apoptotic neurons seen in the CNS of NimC4^KO mutant embryos.

Expression of NimC4^{ΔEMI.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^repo does not rescue the defects in the engulfment of apoptotic neurons seen in the CNS of NimC4^KO mutant embryos.

Expression of NimC4^{ΔNIM1.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^repo does not rescue the defects in the engulfment of apoptotic neurons seen in the CNS of NimC4^KO mutant embryos.

Expression of NimC4^{ΔNIM2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^repo does not rescue the defects in the engulfment of apoptotic neurons seen in the CNS of NimC4^KO mutant embryos.

Expression of NimC4^{ΔNIM3-4.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^repo does not rescue the defects in the engulfment of apoptotic neurons seen in the CNS of NimC4^KO mutant embryos. The glial membranes appear normal in these flies.

Expression of NimC4^{ΔNIM3-4ΔTM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^repo does not rescue the defects in the engulfment of apoptotic neurons seen in the CNS of NimC4^KO mutant embryos.

Expression of NimC4^{ΔNIM3ΔTM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^repo does not rescue the defects in the engulfment of apoptotic neurons seen in the CNS of NimC4^KO mutant embryos.

Expression of NimC4^{ΔNIM4ΔTM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^repo does not rescue the defects in the engulfment of apoptotic neurons seen in the CNS of NimC4^KO mutant embryos.

Expression of NimC4^{ΔTM.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^repo rescues the defects in the engulfment of apoptotic neurons seen in the CNS of NimC4^KO mutant embryos.

(Shklyar et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

NimC4^KO

(Shklover et al., 2015)

SIMU^KO

(Nakano et al., 2019)

nimC4^KO

simu²

(Van Goethem et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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