FlyBase Allele Report: Dmel\Bsg[δ265]

General Information

Symbol

Dmel\Bsg^δ265

Species

D. melanogaster

Name

FlyBase ID

FBal0215571

Feature type

allele

Associated gene

Dmel\Bsg

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Curtin et al., 2005)

Progenitor genotype

Bsg⁰⁶²⁴³

(Curtin et al., 2005)

Cytology

Description

Imprecise excision of the insertion in Bsg⁰⁶²⁴³, resulting in a deletion of approximately 4kb, which includes the first coding exon of the Bsg-265 isoform.

(Curtin et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Munro et al., 2010)

lethal - all die during embryonic stage | recessive

(Curtin et al., 2005)

some die during embryonic stage | recessive

(Curtin et al., 2005)

Phenotype Manifest In

epithelial glial cell

(Munro et al., 2010)

mitochondrion & eye photoreceptor cell | somatic clone

(Curtin et al., 2005)

nerve terminal & eye photoreceptor cell | somatic clone

(Curtin et al., 2005)

neuron & eye photoreceptor cell | somatic clone

(Curtin et al., 2005)

nucleus

(Munro et al., 2010)

photoreceptor neuron

(Munro et al., 2010)

rough endoplasmic reticulum & eye photoreceptor cell | somatic clone

(Curtin et al., 2005)

Detailed Description

Statement

Reference

In Bsg^δ265 mutant eye clones, photoreceptor nuclei are scattered throughout the retina.

In mosaic Bsg^δ265 mutant eye clones the nuclei of the subretinal (including the fenestrated and pseudocartridge glia) and satellite glia remain in their normal positions. However, epithelial glia are displaced within the lamina neuropile.

(Munro et al., 2010)

Approximately 75-80% of homozygotes die as embryos.

Mosaic animals in which the eyes are homozygous for Bsg^δ265 show mislocation of photoreceptor nuclei in 16-50% of cases (average number of misplaced nuclei is 22%).

Rough endoplasmic reticulum (rER) is found misplaced into mutant photoreceptor axon terminals in the lamina of mosaic animals in which the photoreceptors are mutant for Bsg^δ265 in an otherwise wild-type background (the rER is normally confined distally to the photoreceptor cell body in the overlying retina). In addition, mitochondria are also misplaced, accumulating in excessive numbers in the distal portion of photoreceptor terminals. The mutant photoreceptors also show a clear increase in axon terminal size, with profiles that are more than 80% larger in cross-sectional area than controls.

(Curtin et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Bsg^δ265 has neuron & eye photoreceptor cell | somatic clone phenotype, enhanceable by mys^b45

(Curtin et al., 2005)

Suppressed by

Statement

Reference

Bsg^δ265 has neuron & eye photoreceptor cell | somatic clone phenotype, suppressible by Mmus\Bsg^GMR.PC

(Curtin et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the displacement of photoreceptor nuclei phenotype that is seen in the retina of mosaic animals in which the eyes are homozygous for Bsg^δ265 is enhanced if the animals are also hemizygous for mys^b45.

(Curtin et al., 2005)

Xenogenetic Interactions

Statement

Reference

The disruption of photoreceptor nucleus location that is seen in the retinas of mosaic animals in which the eyes are homozygous for Bsg^δ265 is rescued by Mmus\Bsg^GMR.PC.

(Curtin et al., 2005)

Complementation and Rescue Data

Fails to complement

Bsg⁰⁶²⁴³

(Curtin et al., 2005)

Rescued by

Bsg^δ265 is rescued by Bsg^{KRR248MGG.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is rescued by Scer\GAL4^ey.PH/Bsg^{YE246SG.UAS.Tag:V5}

(Munro et al., 2010)

Bsg^δ265 is rescued by Bsg^{P147L.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is rescued by Bsg^{D188N.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is rescued by Bsg^{I178T.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is rescued by Scer\GAL4^ey.PH/Bsg^{PFL228LFT.UAS.Tag:V5}

(Munro et al., 2010)

Bsg^δ265 is rescued by Bsg^UAS.Tag:V5/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is rescued by Bsg^265.GMR

(Curtin et al., 2005)

Partially rescued by

Bsg^δ265 is partially rescued by Bsg^{EG132GV.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is partially rescued by Scer\GAL4^ey.PH/Bsg^{T128A.UAS.Tag:V5}

(Munro et al., 2010)

Bsg^δ265 is partially rescued by Bsg^{WKK64LKT.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is partially rescued by Bsg^{EIE113GFG.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is partially rescued by Bsg^{RVK218LVT.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is partially rescued by Bsg^{Y103S.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Not rescued by

Bsg^δ265 is not rescued by Bsg^{EG132GV.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is not rescued by Scer\GAL4^ey.PH/Bsg^{T128A.UAS.Tag:V5}

(Munro et al., 2010)

Bsg^δ265 is not rescued by Bsg^{EIE113GFG.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is not rescued by Bsg^{RVK218LVT.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is not rescued by Bsg^{Y103S.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is not rescued by Scer\GAL4^ey.PH/Bsg^{E235G.UAS.Tag:V5}

(Munro et al., 2010)

Bsg^δ265 is not rescued by Bsg^{trunc.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is not rescued by Bsg^{DRGEY188AVRES.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Bsg^δ265 is not rescued by Bsg^{W151G.UAS.Tag:V5}/Scer\GAL4^ey.PH

(Munro et al., 2010)

Comments

Expression of Bsg^{KRR248MGG.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced photoreceptor nuclei phenotype.

Expression of Bsg^{YE246SG.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced photoreceptor nuclei phenotype.

Expression of Bsg^{P147L.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced photoreceptor nuclei phenotype.

Expression of Bsg^{D188N.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced photoreceptor nuclei phenotype.

Expression of Bsg^{I178T.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced photoreceptor nuclei phenotype.

Expression of Bsg^{PFL228LFT.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced photoreceptor nuclei phenotype.

Expression of Bsg^{Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced photoreceptor nuclei phenotype.

Expression of Bsg^{EG132GV.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH partially rescues the misplaced photoreceptor nuclei phenotype (leaving 7% misplaced).

Expression of Bsg^{T128A.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH partially rescues the misplaced photoreceptor nuclei phenotype (leaving 10% misplaced).

Expression of Bsg^{WKK64LKT.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH partially rescues the misplaced photoreceptor nuclei phenotype (leaving 10% misplaced).

Expression of Bsg^{EIE113GFG.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH partially rescues the misplaced photoreceptor nuclei phenotype (leaving 10% misplaced).

Expression of Bsg^{RVK218LVT.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH partially rescues the misplaced photoreceptor nuclei phenotype (leaving 10% misplaced).

Expression of Bsg^{Y103S.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH partially rescues the misplaced photoreceptor nuclei phenotype (leaving 10% misplaced).

Expression of Bsg^{W151G.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced photoreceptor nuclei phenotype (leaving 10% misplaced).

Expression of Bsg^{E235G.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced photoreceptor nuclei phenotype (leaving 10% misplaced).

Expression of Bsg^{trunc.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced photoreceptor nuclei phenotype (leaving 10% misplaced).

Expression of Bsg^{DRGEY188AVRES.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced photoreceptor nuclei phenotype (leaving 10% misplaced).

Expression of Bsg^{Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced epithelial glia phenotype.

Expression of Bsg^{KRR248MGG.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced epithelial glia phenotype.

Expression of Bsg^{YE246SG.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced epithelial glia phenotype.

Expression of Bsg^{P147L.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced epithelial glia phenotype.

Expression of Bsg^{D188N.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced epithelial glia phenotype.

Expression of Bsg^{I178T.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced epithelial glia phenotype.

Expression of Bsg^{PFL228LFT.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH rescues the misplaced epithelial glia phenotype.

Expression of Bsg^{EG132GV.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced epithelial glia phenotype.

Expression of Bsg^{T128A.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced epithelial glia phenotype.

Expression of Bsg^{WKK64LKT.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH partially rescues the misplaced epithelial glia phenotype.

Expression of Bsg^{EIE113GFG.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced epithelial glia phenotype.

Expression of Bsg^{RVK218LVT.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced epithelial glia phenotype.

Expression of Bsg^{Y103S.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced epithelial glia phenotype.

Expression of Bsg^{E235G.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced epithelial glia phenotype.

Expression of Bsg^{trunc.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced epithelial glia phenotype.

Expression of Bsg^{W151G.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced epithelial glia phenotype.

Expression of Bsg^{DRGEY188AVRES.Scer\UAS.T:SV5\V5} in Bsg^δ265 mosaic eye clones under the control of Scer\GAL4^ey.PH fails to rescue the misplaced epithelial glia phenotype.

(Munro et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Bsg²⁶⁵

(Hunter et al., 2020)

Bsg^δ265

bsg^δ265

(Shrestha et al., 2021, Munro et al., 2010, Curtin et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (4)

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