FlyBase Allele Report: Dmel\Dnah3[MB05004]

General Information

Symbol

Dmel\Dnah3^MB05004

Species

D. melanogaster

Name

FlyBase ID

FBal0212592

Feature type

allele

Associated gene

Dmel\Dnah3

Associated Insertion(s)

Mi{ET1}Dnah3^MB05004

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Karak et al., 2015)

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{ET1}Dnah3^MB05004

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Mi{ET1}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:4,865,168..4,865,168 [+]

(Karak et al., 2015, Bellen, 2008.1.15)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception

(Karak et al., 2015)

male sterile

(Karak et al., 2015)

Phenotype Manifest In

spermatozoon

(Karak et al., 2015)

Detailed Description

Statement

Reference

Electrical sound responses of Johnston's organ neurons are virtually abolished in Dnah3^MB05004 mutant flies. Mechanical amplification is excessive compared to controls.

(Karak et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Dnah3^MB05004 has abnormal auditory perception phenotype, non-suppressible by nan^dy5

(Karak et al., 2015)

Suppressor of

Statement

Reference

Dnah3^MB05004 is a suppressor | partially of abnormal auditory perception phenotype of nan^dy5

(Karak et al., 2015)

Dnah3^MB05004 is a suppressor of abnormal auditory perception phenotype of iav^K1

(Karak et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

As in either mutant alone, nan^dy5 Dnah3^MB05004 double mutants entirely lack sound-evoked electrical potentials. Mechanical amplification is excessive in the double mutants, similar to Dnah3^MB05004 mutants, substantially lower than in nan^dy5 mutants alone.

No gross morphological defects are seen in the Johnston's organ neurons in Dnah3^MB05004 mutant flies and the outer dynein arms in the proximal region of the cilium persist and seem structurally uncompromised.

Sperm from Dnah3^MB05004/Df(3L)BSC371 mutant males shows a significant reduction in sperm competition compared with controls.

(Karak et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Dnah3^MB05004 is rescued by Dnah3^+tWT

(Karak et al., 2015)

Comments

Expression of Dnah3^+tWT rescues the sound-evoked electrical and mechanical response defects seen in Dnah3^MB05004 mutant flies.

(Karak et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24844

w¹¹¹⁸; Mi{ET1}Dnah3^MB05004/TM6C, Sb¹

Notes on Origin

Discoverer

Separable from: a secondary uncharacterised mutation that causes male fertility and sperm motility defects.

(Karak et al., 2015)

Comments

Precise excision of the Mi{ET1}Dnah3^MB05004 element restores the sound-evoked electrical and mechanical response defects seen in Dnah3^MB05004 mutant flies.

(Karak et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG17150^MB05004

(Bellen, 2008.1.15)

Dmdnah3¹

(Karak et al., 2015)

Dnah3^MB05004

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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