UASt regulatory sequences drive expression of an inverted repeat.
The expression of sliGD5822 under the control of Scer\GAL4Mef2.247 leads to a decrease in filopodial and lamellopodial extensions in embryonic heart cardioblasts, as compared to controls.
Expression of sliGD5822 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4GMR.PU has no effect on adult eye appearance.
Adults expressing sliGD5822 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression of sliGD5822 using Scer\GAL4sli.PS results in the dendrites of the motoneuron labelled by Scer\GAL4VGlut-VGN9281 shifting into intermediate and medial neuropil territories with several branches projecting to and terminating at the midline. (Expression of sliGD5822 via Scer\GAL4VGlut-VGN9281 alone results in a wild type distribution of dendrites.)
Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 0%, 10-20% or 20-30% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 30-40% or 40-50% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in duplication of bristle hairs on the notum in 0% or 0-10% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in external sensory organs consisting of empty or multiple sockets in 0% or 10-20% of the Scer\GAL4pnr-MD237 expression domain of the notum, depending on the insertion line used.
sliGD5822, Scer\GAL4GMR.PU is an enhancer of visible | adult stage phenotype of Hsap\MAPTV337M.UAS, Scer\GAL4GMR.PU
sliGD5822, Scer\GAL4GMR.PU is an enhancer of eye | adult stage phenotype of Hsap\MAPTV337M.UAS, Scer\GAL4GMR.PU
The moderate rough eye phenotype and reduced adult eye size characteristic for flies expressing Scer\GAL4GMR.PU under the control of Hsap\MAPTV337M.Scer\UAS is significantly worsened by co-expression of sliGD5822 (together with UAS-Dicer2 to enhance RNAi efficiency), exacerbating the rough appearance.