FB2024_03 , released June 25, 2024
Allele: Dmel\sliGD5822
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General Information
Symbol
Dmel\sliGD5822
Species
D. melanogaster
Name
FlyBase ID
FBal0210610
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-slit-RNAi
Key Links
Genomic Maps

Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Carried in construct
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of sliGD5822 under the control of Scer\GAL4Mef2.247 leads to a decrease in filopodial and lamellopodial extensions in embryonic heart cardioblasts, as compared to controls.

Expression of sliGD5822 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4GMR.PU has no effect on adult eye appearance.

Adults expressing sliGD5822 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of sliGD5822 using Scer\GAL4sli.PS results in the dendrites of the motoneuron labelled by Scer\GAL4VGlut-VGN9281 shifting into intermediate and medial neuropil territories with several branches projecting to and terminating at the midline. (Expression of sliGD5822 via Scer\GAL4VGlut-VGN9281 alone results in a wild type distribution of dendrites.)

Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 0%, 10-20% or 20-30% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 30-40% or 40-50% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in duplication of bristle hairs on the notum in 0% or 0-10% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in external sensory organs consisting of empty or multiple sockets in 0% or 10-20% of the Scer\GAL4pnr-MD237 expression domain of the notum, depending on the insertion line used.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Phenotype Manifest In
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The moderate rough eye phenotype and reduced adult eye size characteristic for flies expressing Scer\GAL4GMR.PU under the control of Hsap\MAPTV337M.Scer\UAS is significantly worsened by co-expression of sliGD5822 (together with UAS-Dicer2 to enhance RNAi efficiency), exacerbating the rough appearance.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Linkouts
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (14)