FlyBase Allele Report: Dmel\mad2[GD16427]

General Information

Symbol

Dmel\mad2^GD16427

Species

D. melanogaster

Name

FlyBase ID

FBal0210040

Feature type

allele

Associated gene

Dmel\mad2

Associated Insertion(s)

Carried in Construct

P{GD16427}

Also Known As

UAS-mad2^RNAi, UAS-mad2-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD16427}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD16427

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of microcephaly

CEA

(Islam et al., 2024)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | third instar larval stage, with Scer\GAL4^en.PU

(Liu et al., 2015)

lethal, with Scer\GAL4^da.PU

(Liu et al., 2015)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

wing disc | third instar larval stage, with Scer\GAL4^en-e16E

(Liu et al., 2016)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^en.PU

(Liu et al., 2015)

Detailed Description

Statement

Reference

Expression of mad2^GD16427 under the control of Scer\GAL4^en-e16E to induce chromosome instability in the proliferative third instar larval wing disc tissue results in a marked increase in autophagy (visualized by autophagy markers Atg8 and LysoTracker) and oxidative stress (elevated levels of ROS), however the apoptotic activity (visualized using [*]Casp3) is not significantly increased.

(Liu et al., 2016)

Under 30[o]C temperature conditions, the expression of mad2^GD16427 under the control of Scer\GAL4^da.PU induces lethality before adulthood.

Under 30[o]C of temperature conditions, expression of mad2^GD16427 driven by Scer\GAL4^en.PU leads to a significant increase in aneuploidy in the posterior compartment of third instar larval wing discs, as compared to controls.

(Liu et al., 2015)

Expression of mad2^GD16427 in the posterior compartment of the wing disc, under the control of either Scer\GAL4^en.PU or Scer\GAL4^hh.PU, induces chromosome instability but does not lead to an increase in cell death.

(Wong et al., 2014)

Adults expressing mad2^GD16427 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, suppressible | partially by dl^JF02825/Scer\GAL4^da.PU

(Liu et al., 2015)

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, suppressible | partially by Scer\GAL4^da.PU/spz^HM05024

(Liu et al., 2015)

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, suppressible | partially by Scer\GAL4^da.PU/spz^HMS01178

(Liu et al., 2015)

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, suppressible | partially by Scer\GAL4^da.PU/pll^HMS01213

(Liu et al., 2015)

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, suppressible | partially by Scer\GAL4^da.PU/cact^HMS00084

(Liu et al., 2015)

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, suppressible | partially by Scer\GAL4^da.PU/Tl^GL00474

(Liu et al., 2015)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Dark^VDRC.cUa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Dark^VDRC.cUa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Scer\GAL4^hh.PU/hid^VDRC.cUa

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Scer\GAL4^en.PU/hid^VDRC.cUa

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Debcl^VDRC.cUa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Debcl^VDRC.cUa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by CycB[+]/CycB²

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by CycB[+]/CycB²

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Myt1^UAS.cPa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by Myt1^UAS.cPa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, Tak1^KK108611, mad2^GD16427 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^KK108611, mad2^GD16427 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, Tak1^NIG.1388R, mad2^GD16427 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^NIG.1388R, mad2^GD16427 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, mad2^GD16427, mbt^GD9608 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, mbt^GD9608 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, non-suppressible by Toll-4^HM05029/Scer\GAL4^da.PU

(Liu et al., 2015)

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, non-suppressible by pll^GL00150/Scer\GAL4^da.PU

(Liu et al., 2015)

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, non-suppressible by Tollo^HM05005/Scer\GAL4^da.PU

(Liu et al., 2015)

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, non-suppressible by MstProx^HM05012, Scer\GAL4^da.PU

(Liu et al., 2015)

Scer\GAL4^da.PU, mad2^GD16427 has lethal phenotype, non-suppressible by Scer\GAL4^da.PU/Toll-7^HM05230

(Liu et al., 2015)

Other

Statement

Reference

Pepck1^GD9451, Scer\GAL4^en-e16E, mad2^GD16427 has increased cell death | third instar larval stage phenotype

(Hussain et al., 2017)

Atg1^GD7149, Scer\GAL4^en-e16E, mad2^GD16427 has increased cell death | third instar larval stage phenotype

(Liu et al., 2016)

Atg18a^GD12342, Scer\GAL4^en-e16E, mad2^GD16427 has increased cell death | third instar larval stage phenotype

(Liu et al., 2016)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, hep^GD1461, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, hep^NIG.2190R, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, hep^NIG.2190R, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, Tak1^KK108611, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^KK108611, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, Tak1^NIG.1388R, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^NIG.1388R, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, mad2^GD16427, mbt^GD9608 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, mbt^GD9608 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, mad2^GD16427, msn^KK108948 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, msn^KK108948 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, mad2^GD16427, msn^NIG.16973R has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, msn^NIG.16973R has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, foxo^GD4348, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, foxo^GD4348, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

14-3-3ζ^GD16467, Scer\GAL4^hh.PU, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

14-3-3ζ^GD16467, Scer\GAL4^en.PU, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Jra^KK100582, Scer\GAL4^hh.PU, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Jra^KK100582, Scer\GAL4^en.PU, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^GD10555, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^GD10555, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Dark^VDRC.cUa, Scer\GAL4^hh.PU, bsk^GD10555, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Dark^VDRC.cUa, Scer\GAL4^en.PU, bsk^GD10555, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, hep^GD1461, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by Debcl^VDRC.cUa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, Tak1^KK108611, mad2^GD16427 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^KK108611, mad2^GD16427 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, Tak1^NIG.1388R, mad2^GD16427 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^NIG.1388R, mad2^GD16427 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, mad2^GD16427, mbt^GD9608 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, mbt^GD9608 has wing disc phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by Dark^VDRC.cUa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by Dark^VDRC.cUa, Scer\GAL4^en.PU

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by Scer\GAL4^hh.PU/hid^VDRC.cUa

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by Scer\GAL4^en.PU/hid^VDRC.cUa

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype, suppressible by Debcl^VDRC.cUa, Scer\GAL4^hh.PU

(Wong et al., 2014)

Other

Statement

Reference

Pepck1^GD9451, Scer\GAL4^en-e16E, mad2^GD16427 has wing disc posterior compartment | third instar larval stage phenotype

(Hussain et al., 2017)

Atg1^GD7149, Scer\GAL4^en-e16E, mad2^GD16427 has wing disc | third instar larval stage phenotype

(Liu et al., 2016)

Atg18a^GD12342, Scer\GAL4^en-e16E, mad2^GD16427 has wing disc | third instar larval stage phenotype

(Liu et al., 2016)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has wing phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, hep^GD1461, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, hep^GD1461, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, hep^NIG.2190R, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, hep^NIG.2190R, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, Tak1^KK108611, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^KK108611, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, Tak1^NIG.1388R, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, Tak1^NIG.1388R, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, mad2^GD16427, mbt^GD9608 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, mbt^GD9608 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, mad2^GD16427, msn^KK108948 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, msn^KK108948 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, mad2^GD16427, msn^NIG.16973R has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, msn^NIG.16973R has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^hh.PU, foxo^GD4348, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, bsk^KK108156, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, foxo^GD4348, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

14-3-3ζ^GD16467, Scer\GAL4^hh.PU, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

14-3-3ζ^GD16467, Scer\GAL4^en.PU, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Jra^KK100582, Scer\GAL4^hh.PU, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Jra^KK100582, Scer\GAL4^en.PU, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Posterior compartments of third instar wing discs expressing both Pepck^GD9451 and mad2^GD16427 under the control of Scer\GAL4^en-e16E exhibit a significant increase in cell death, as compared to controls.

(Hussain et al., 2017)

Co-expression of mad2^GD16427 with either Atg1^GD7149 or Atg18a^GD12342 under the control of Scer\GAL4^en-e16E results in a dramatic increase in cell death (visualized by [*]Casp3 staining) in third instar larval wing discs.

(Liu et al., 2016)

Under 30[o]C temperature conditions, the Scer\GAL4^da.PU-driven expression of mad2^GD16427 alone, or together with either Toll-4^HM05029, pll^GL00150, Tollo^HM05005, MstProx^HM05012, or Toll-7^HM05230, induces lethality before adulthood; there are a few adult survivors when mad2^GD16427 is expressed together with either dl^JF02825, spz^HM05024, spz^HMS01178, pll^HMS01213, cact^HMS00084 or Tl^GL00474.

(Liu et al., 2015)

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when bsk^KK108156 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. This leads to an increase in cell death. Consistent with high levels of cell death and consequent loss of tissue, adult wings show notching when JNK signalling is reduced in a chromosome instability background (through expression of mad2^GD16427).

Expression of bsk^KK108156 in the posterior wing disc compartment of flies expressing mad2^GD16427 (with both lines under the control of either Scer\GAL4^en.PU or Scer\GAL4^hh.PU) results in both chromosome instability and high levels of cell death. This cell death is inhibited by the addition of BacA\p35^Scer\UAS.cHa.

Knockdown of Dark, through expression of Dark^VDRC.cUa results in significantly reduced levels of cell death in imaginal wing disc cells expressing bsk^GD10555 and mad2^GD16427.

Cell death induced by bsk knockdown in cells exhibiting chromosomal instability (through expression of mad2^GD16427) is reduced by lengthening G[[2]] through a CycB² heterozygous background.

Cell death induced by bsk knockdown in cells exhibiting chromosomal instability (through expression of mad2^GD16427) is reduced by lengthening G[[2]] by Myt1^Scer\UAS.cPa overexpression.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when bsk^GD10555 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. This leads to an increase in cell death.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when hep^GD1461 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. This leads to an increase in cell death.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when hep^NIG.2190R is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. This leads to an increase in cell death.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when slpr^GD9771 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when slpr^KK100726 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when Tak1^KK108611 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. This is concomitant with an increase in the level of cell death. Consistent with high levels of cell death and consequent loss of tissue, adult wings show notching when JNK signalling is reduced in a chromosome instability background (through expression of mad2^GD16427).

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when Tak1^NIG.1388R is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant increase in cell death in these double mutants.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when mbt^GD9608 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant increase in cell death in these double mutants.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when msn^KK108948 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant increase in cell death in these double mutants. Consistent with high levels of cell death and consequent loss of tissue, adult wings show notching when JNK signalling is reduced in a chromosome instability background (through expression of mad2^GD16427).

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when msn^NIG.16973R is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant increase in cell death in these double mutants.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when GckIII^GD16418 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when GckIII^KK101915 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU.

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when foxo^GD4348 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant significant increase in cell death in these double mutants. Consistent with high levels of cell death and consequent loss of tissue, adult wings show notching when JNK signalling is reduced in a chromosome instability background (through expression of mad2^GD16427).

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when 14-3-3ζ^GD16467 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant significant increase in cell death in these double mutants. Consistent with high levels of cell death and consequent loss of tissue, adult wings show notching when JNK signalling is reduced in a chromosome instability background (through expression of mad2^GD16427).

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when Jra^KK100582 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant significant increase in cell death in these double mutants.

Knockdown of hid through expression of hid^VDRC.cUa under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU results in significantly reduced levels of cell death in imaginal discs expressing bsk^KK108156 and mad2^GD16427.

Knockdown of Debcl through expression of Debcl^VDRC.cUa under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU results in significantly reduced levels of cell death in imaginal discs expressing bsk^KK108156 and mad2^GD16427.

Knockdown of Dark through expression of Dark^VDRC.cUa under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU results in significantly reduced levels of cell death in imaginal discs expressing bsk^KK108156 and mad2^GD16427.

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU, in a mad2^GD16427 mutant background, show little cell death.

The cell death and DNA damage seen in imaginal disc cells expressing stg^GD8177, under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU is strongly enhanced by expression of mad2^GD16427, which induced chromosomal instability.

Shortening the G[[1]] cell cycle phase in imaginal disc cells, through expression of CycE^Scer\UAS.cRa under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU, does not result in an increase in DNA damage and cell death when chromosomal instability is induced by co-expression of mad2^GD16427.

(Wong et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU, in a mad2^GD16427 mutant background, results in little cell death.

(Wong et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v47919

w¹¹¹⁸; P{GD16427}v47919

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mad2^GD16427

Name Synonyms

Secondary FlyBase IDs

References (11)

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