UASt regulatory sequences drive expression of an inverted repeat.
Expression of htlGD14457 under the control of Scer\GAL4SPARC-MI00329-GAL4 results in reduced number of ensheathing glial cells in the developing antennal lobes, alters the pattern the ensheathing glia follow to wrap the antennal lobe glomeruli and disrupts the glomerular compartmentalization during pupal development.
Clonal expression of htlGD14457 under the control of Scer\GAL4-FRT.alrm in astrocytes results in reduced cell body size and a reduction in the volume of astrocyte processes infiltrated into the neuropil compared to controls.
Expression of htlGD14457 in retinal basal glial (RBG) cell clones under the control of Scer\GAL4repo results in a reduction in RBG cell number, loss of differentiation and lack of migration in third instar larvae.
Adults expressing htlGD14457 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in early larval lethality or late larval lethality.
Expression under the control of Scer\GAL4Mef2.PR results in split larval body wall muscles.
Expression under the control of Scer\GAL4Mef2.PR results in sarcomeres with discontinuous Z-lines in the larval body wall muscles.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 0% or 90-100% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 0% or 30-40% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Scer\GAL4SPARC-MI00329-GAL4, htlGD14457 has adult ensheathing glial cell | P-stage phenotype, suppressible by BacA\p35UAS.cHa, Scer\GAL4SPARC-MI00329-GAL4
Scer\GAL4SPARC-MI00329-GAL4, htlGD14457 has antennal lobe glomerulus | P-stage phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4SPARC-MI00329-GAL4
The reduced number of ensheathing glia in the developing antennal lobes at late pupal stages induced by Scer\GAL4SPARC-MI00329-GAL4-controlled expression of htlGD14457 is suppressed by co-expression of BacA\p35Scer\UAS.cHa, this however does not rescue either the altered wrapping pattern of the ensheathing glial cells or the disrupted glomerular compartmentalization.