UASt regulatory sequences drive expression of an inverted repeat.
Expression of Su(z)2GD16388 under the control of Scer\GAL4C587 (together with tub-Gal80[ts] to limit the time of expression to adulthood) does not cause any obvious defects in germaria morphology and development.
Adults expressing Su(z)2GD16388 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has increased cell number | female | adult stage phenotype, suppressible | temperature conditional by bamhs.PO
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has neoplasia | female | adult stage phenotype, suppressible | temperature conditional by bamhs.PO
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has increased cell number | female | adult stage phenotype, suppressible | partially by dppJF01090, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has neoplasia | female | adult stage phenotype, suppressible | partially by dppJF01090, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has increased cell number | female | adult stage phenotype, suppressible | partially by dppJF01091, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has neoplasia | female | adult stage phenotype, suppressible | partially by dppJF01091, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has increased cell number | female | adult stage phenotype, suppressible | partially by dppJF01677, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has neoplasia | female | adult stage phenotype, suppressible | partially by dppJF01677, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has increased cell number | female | adult stage phenotype, suppressible | partially by dpphr56/dpp[+]
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has neoplasia | female | adult stage phenotype, suppressible | partially by dpphr56/dpp[+]
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has increased cell number | female | adult stage phenotype, suppressible | partially by dppe90/dpp[+]
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has neoplasia | female | adult stage phenotype, suppressible | partially by dppe90/dpp[+]
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has increased cell number | female | adult stage phenotype, suppressible | partially by brmHM04019, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has neoplasia | female | adult stage phenotype, suppressible | partially by brmHM04019, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has increased cell number | female | adult stage phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has neoplasia | female | adult stage phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has increased cell number | female | adult stage phenotype
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has neoplasia | female | adult stage phenotype
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has germarium phenotype, suppressible | partially by brmHM04019, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has germarium phenotype, suppressible | temperature conditional by bamhs.PO
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has germarium phenotype, suppressible | partially by dppJF01090, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has germarium phenotype, suppressible | partially by dppJF01091, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has germarium phenotype, suppressible | partially by dppJF01677, Scer\GAL4C587
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has germarium phenotype, suppressible | partially by dpphr56/dpp[+]
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has germarium phenotype, suppressible | partially by dppe90/dpp[+]
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has germarium phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4C587
Su(z)2GD16388, Scer\GAL4GMR.PF is an enhancer of eye phenotype of Hsap\MAPTR406W.UAS, Scer\GAL4GMR.PF
Su(z)2GD16388, Scer\GAL4GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PF
Su(z)2GD16388, Scer\GAL4GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PF
PscGD4481, Scer\GAL4C587, Su(z)2GD16388 has germarium phenotype
Simultaneous expression of both PscGD4481 and Su(z)2GD16388 under the control of Scer\GAL4C587 (together with tub-Gal80[ts] to limit the time of expression to adulthood) results in tumorigenic accumulation of spectrosome containing germline stem cell-like (GSC-like) cells (which increase in number with the time of expression) in female germaria, however no increase in apoptosis is observed. Expression of bamhs.PO (induced by 1hr heat-shock once a day for 5 consecutive days) induces differentiation of the tumor cells. The accumulation of GSC-like cells is also significantly suppressed by co-expression of any of the following: dppJF01090, dppJF01091, dppJF01677 or brmHM04019 or by combination with a single copy of either dpphr56 or dppe90.
The tumorigenic phenotype (accumulation of germline stem cell-like undifferentiated cells) observed in female germaria upon simultaneous expression of PscGD4481 and Su(z)2GD16388 under the control of Scer\GAL4C587 (together with tub-Gal80[ts] to limit the time of expression to adulthood) is not suppressed by co-expression of BacA\p35Scer\UAS.cHa.