FlyBase Allele Report: Dmel\Hrb27C[GD6964]

General Information

Symbol

Dmel\Hrb27C^GD6964

Species

D. melanogaster

Name

FlyBase ID

FBal0207319

Feature type

allele

Associated gene

Dmel\Hrb27C

Associated Insertion(s)

Carried in Construct

P{GD6964}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6964}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6964

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Voßfeldt et al., 2012)

ameliorates neurodegenerative disease

modeled by TER94^R152H.UAS.R

(Ritson et al., 2010)

modeled by TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | somatic clone | third instar larval stage, with Scer\GAL4^αTub84B.PL

(Mach et al., 2018)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Bruckert et al., 2015)

abnormal size | adult stage, with Scer\GAL4^ey.PH

(Mach et al., 2018)

abnormal size | adult stage, with Scer\GAL4^mirr-DE

(Mach et al., 2018)

abnormal size | third instar larval stage, with Scer\GAL4^hh-Gal4

(Mach et al., 2018)

increased cell death | third instar larval stage, with Scer\GAL4^ptc-559.1

(Mach et al., 2018)

lethal, with Scer\GAL4^elav.PU

(Appocher et al., 2017)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal - all die during pupal stage, with Scer\GAL4^hh-Gal4

(Mach et al., 2018)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^ey.PH

(Mach et al., 2018)

visible | adult stage, with Scer\GAL4^mirr-DE

(Mach et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing Hrb27C^GD6964 under the control of Scer\GAL4^ey.PH results in a reduced overall eye size, while expression under the control of Scer\GAL4^mirr-DE leads to an almost complete ablation of the eye dorsal compartment in adults. Expression under the control of Scer\GAL4^hh-Gal4 causes a severe reduction of the wing disc posterior compartment in third instar larvae.

Somatic clones expressing Hrb27C^GD6964 under the control of Scer\GAL4^αTub84B.PL in eye discs of third instar larvae display reduced cell proliferation (determined by EdU incorporation).

Expressing Hrb27C^GD6964 under the control of Scer\GAL4^ptc-559.1 results in increased cell death compared to controls in wing discs of third instar larvae, while expression under the control of Scer\GAL4^hh-Gal4 causes a reduction in cell density in the posterior compartment. Expression under the control of Scer\GAL4^ptc-559.1 has no effect on the size of the anterior-posterior compartment boundary of the wing disc.

(Mach et al., 2018)

Expression of Hrb27C^GD6964 under the control of Scer\GAL4^GMR.PU has no obvious effect on adult eye morphology, whereas expression driven by Scer\GAL4^elav.PU is lethal.

(Appocher et al., 2017)

Expression of Hrb27C^GD6964 under the control of Scer\GAL4^ey-OK107 leads to a general decrease in mushroom body (MB) lobe volume, MB αβ lobe defects (asymmetric, shorter or missing lobes) and exhibit abnormal overextension of dorsal lobes when compared to controls. The proportion of MBs with axon overextension defects and the proportion of MBs with asymmetric or truncated α or β lobes are higher in females than in males.

(Bruckert et al., 2015)

Adults expressing Hrb27C^GD6964 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Hrb27C^GD6964 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD6964} insertion line used.

(Neely et al., 2010)

Expression of Hrb27C^GD6964 in the developing eye under the control of Scer\GAL4^GMR.PF does not affect eye development.

(Ritson et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 10-20% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 30-40% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 40-50% or 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6964

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hrb27C^GD6964, Scer\GAL4^dpp.blk1 is a suppressor of abnormal size | third instar larval stage phenotype of Rnor\Prkcz^UAS.act, Scer\GAL4^dpp.blk1

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^ptc-559.1 is a suppressor of abnormal size | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, hpo^KK101704

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^ptc-559.1 is a suppressor of abnormal size | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, wts^KK101055

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^ptc-559.1 is a suppressor of abnormal size | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, wts^KK101055, yki^{UAS.cOa.Tag:V5}

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^ptc-559.1 is a suppressor | partially of abnormal size | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, yki^{S168A.UAS.Tag:V5}

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^ptc-559.1 is a suppressor | partially of abnormal size | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, sd^{GA.UAS.Tag:HA}

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Rnor\Prkcz^UAS.act, Scer\GAL4^GMR.PU

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^GMR.PU is a suppressor | partially of abnormal size | adult stage phenotype of Rnor\Prkcz^UAS.act, Scer\GAL4^GMR.PU

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Hrb27C^GD6964, Scer\GAL4^GMR.PU is a suppressor | partially of melanotic mass phenotype | adult stage phenotype of Scer\GAL4^GMR.PU, TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Hrb27C^GD6964, Scer\GAL4^GMR.PU is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4^GMR.PU, TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Hrb27C^GD6964, Scer\GAL4^GMR.long is a suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Voßfeldt et al., 2012)

Hrb27C^GD6964, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, TER94^R152H.UAS.R

(Ritson et al., 2010)

Other

Statement

Reference

Hrb27C^GD6964, Scer\GAL4^elav.PU, TBPH[+]/TBPH^Δ23, TBPH^RNAi.UAS.cUa has lethal phenotype

(Appocher et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hrb27C^GD6964, Scer\GAL4^ptc-559.1 is a suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, hpo^KK101704

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^ptc-559.1 is a suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, wts^KK101055

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^ptc-559.1 is a suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, wts^KK101055, yki^{UAS.cOa.Tag:V5}

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^ptc-559.1 is a suppressor | partially of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, yki^{S168A.UAS.Tag:V5}

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Rnor\Prkcz^UAS.act, Scer\GAL4^GMR.PU

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^dpp.blk1 is a suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Rnor\Prkcz^UAS.act, Scer\GAL4^dpp.blk1

(Mach et al., 2018)

Hrb27C^GD6964, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Hrb27C^GD6964, Scer\GAL4^GMR.PU is a suppressor | partially of ommatidium | adult stage phenotype of Scer\GAL4^GMR.PU, TBPH^UAS.Tag:FLAG

(Appocher et al., 2017)

Hrb27C^GD6964, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.long

(Voßfeldt et al., 2012)

Hrb27C^GD6964, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Voßfeldt et al., 2012)

Hrb27C^GD6964, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, TER94^R152H.UAS.R

(Ritson et al., 2010)

Hrb27C^GD6964, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, TER94^R152H.UAS.R

(Ritson et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Animals expressing Hrb27C^GD6964 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^elav.PU in either wild-type or TBPH^Δ23/+; TBPH^{dsRNA.Scer\UAS.cUa} background are lethal.

The eye degeneration phenotype (appearance of melanotic spots, fusion of ommatidia) characteristic for adult flies expressing TBPH^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU is strongly suppressed by co-expression of Hrb27C^GD6964.

(Appocher et al., 2017)

Expression of Hrb27C^GD6964 in the developing eye dominantly suppresses the eye degeneration phenotype found upon expression of TER94^{R152H.Scer\UAS.R} (with both transgenes driven by Scer\GAL4^GMR.PF). This is concurrent with a significant reduction in the blinded phenotypic severity score.

(Ritson et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v16040