FlyBase Allele Report: Dmel\DCTN1-p150[GD1455]

General Information

Symbol

Dmel\DCTN1-p150^GD1455

Species

D. melanogaster

Name

FlyBase ID

FBal0207114

Feature type

allele

Associated gene

Dmel\DCTN1-p150

Associated Insertion(s)

Carried in Construct

P{GD1455}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1455}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1455

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis

CEA

(Borg et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | larval stage, with Scer\GAL4^Hml.PG

(Howell et al., 2012)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

decreased rate of adult locomotory behavior, with Scer\GAL4^Mef2.PU

(Borg et al., 2023)

decreased rate of adult locomotory behavior | progressive, with Scer\GAL4^elav.PU

(Borg et al., 2023)

decreased rate of flight behavior, with Scer\GAL4^Mef2.PU

(Borg et al., 2023)

decreased rate of flight behavior | progressive, with Scer\GAL4^elav.PU

(Borg et al., 2023)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal - all die before end of P-stage, with Scer\GAL4^Act5C.PU

(Borg et al., 2023)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

short lived, with Scer\GAL4^elav.PU

(Borg et al., 2023)

some die during P-stage, with Scer\GAL4^Act5C.PU

(Borg et al., 2023)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^elav.PU

(Borg et al., 2023)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^Mef2.PU

(Borg et al., 2023)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

axon, with Scer\GAL4^Toll-6-D42

(Butzlaff et al., 2015)

border follicle cell, with Scer\GAL4^upd1.PU

(Van De Bor et al., 2011)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^Act5C.PU

(Borg et al., 2023)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

NMJ bouton | decreased number | larval stage, with Scer\GAL4^Act5C.PU

(Borg et al., 2023)

Detailed Description

Statement

Reference

DCTN1-p150 silencing in segmental nerves of L3 larvae (DCTN1-p150^GD1455, Scer\GAL4^D42) significantly reduces the rate of mitochondria transported in the retrograde direction, but has no effect on retrograde speed or anterograde speed/rate.

Scer\GAL4^elav-C155-mediated co-expression of DCTN1-p150^GD1455 has no apparent effect on eclosion rates.

(Butzlaff et al., 2015)

Larvae expressing Gl^GD1455 under the control of Scer\GAL4^Hml.PG show reduced encapsulation (10%) of eggs when parasitised by the avirulent L. boulardi wasp strain G486. Plasmatocytes fully cover the egg, while lamellocytes partially cover it.

(Howell et al., 2012)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Expression of Gl^GD1455 under the control of Scer\GAL4^upd results in a reduction in the number of outer border follicle cells compared to wild type and also results in defects in border follicle cell migration. Expression under the control of Scer\GAL4^slbo.2.6 does not cause these phenotypes.

(Van De Bor et al., 2011)

Adults expressing DCTN1-p150^GD1455 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1455

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

DCTN1-p150^GD1455, Hsap\MAPT^R406W.UAS, Scer\GAL4^elav-C155 has partially lethal phenotype

(Butzlaff et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

DCTN1-p150^GD1455, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Enhancer of

Statement

Reference

DCTN1-p150^GD1455, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Suppressor of

Statement

Reference

DCTN1-p150^GD1455, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

Other

Statement

Reference

DCTN1-p150^GD1455, Hsap\MAPT^R406W.UAS, Scer\GAL4^elav-C155 has axon | larval stage phenotype

(Butzlaff et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Scer\GAL4^elav-C155-mediated expression of Hsap\MAPT^{R406W.Scer\UAS} in combination with silencing of DCTN1-p150 (DCTN1-p150^GD1455) results in axonal swellings in larval segmental nerves.

Scer\GAL4^elav-C155-mediated co-expression of Hsap\MAPT^{R406W.Scer\UAS} with DCTN1-p150^GD1455 strongly reduces eclosion rates.

(Butzlaff et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v3785

w¹¹¹⁸; P{GD1455}v3785/CyO

Notes on Origin