FlyBase Allele Report: Dmel\GATAe[GD4152]

General Information

Symbol

Dmel\GATAe^GD4152

Species

D. melanogaster

Name

FlyBase ID

FBal0207072

Feature type

allele

Associated gene

Dmel\GATAe

Associated Insertion(s)

Carried in Construct

P{GD4152}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4152}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4152

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

adult posterior midgut epithelium, with Scer\GAL4^NP6267

(Okumura et al., 2016)

adult posterior midgut epithelium | somatic clone, with Scer\GAL4^Act5C.PI

(Okumura et al., 2016)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of GATAe^GD4152 under the control of Scer\GAL4^NP6267 (using tub-Gal80[ts] to limit the time of expression) leads to a thin midgut tube compared to wild-type and loss of esg-positive cells as well as defects in their morphology and position (cells are spherical rather than pyramidal, detached from the base membrane) in the adult posterior midgut. Somatic MARCM clones expressing GATAe^GD4152 under the Scer\GAL4^Act5C.PI driver fail to grow compared to controls.

(Okumura et al., 2016)

Adults expressing GATAe^GD4152 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing GATAe^GD4152 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in mild thorax closure defects.

Expression under the control of Scer\GAL4^pnr-MD237 may result in an enlarged notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4152

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

GATAe^GD4152, Scer\GAL4^NP6267 has adult posterior midgut epithelium phenotype, non-suppressible by Diap1^UAS.Tag:MYC/BacA\p35^UAS.cUa, Scer\GAL4^NP6267

(Okumura et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The loss of esg-positive cells as well as their aberrant morphology in the adult posterior midgut expressing GATAe^GD4152 under the control of Scer\GAL4^NP6267 (using tub-Gal80[ts] to limit the time of expression) cannot be rescued by the combined co-expression of both Diap1^{Scer\UAS.T:Hsap\MYC} and BacA\p35^Scer\UAS.cUa.

(Okumura et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v10418

w¹¹¹⁸; P{GD4152}v10418

v10420

w¹¹¹⁸; P{GD4152}v10420