FlyBase Allele Report: Dmel\Fer1HCH[GD4853]

General Information

Symbol

Dmel\Fer1HCH^GD4853

Species

D. melanogaster

Name

FlyBase ID

FBal0207025

Feature type

allele

Associated gene

Dmel\Fer1HCH

Associated Insertion(s)

Carried in Construct

P{GD4853}

Also Known As

fer1HCH RNAi, UAS-Fer1HCH-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4853}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4853

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^elav-C155

(Xiao et al., 2019)

abnormal size | adult stage, with Scer\GAL4^GMR.PFa

(Xiao et al., 2019)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal, with Scer\GAL4^tim.PE

(Mandilaras and Missirlis, 2012)

lethal | nutrition conditional, with Scer\GAL4^NP3084

(Xiao et al., 2019)

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal - all die during second instar larval stage, with Scer\GAL4^Cg.PA

(Xiao et al., 2019)

lethal - all die during second instar larval stage, with Scer\GAL4^da.G32

(Xiao et al., 2019)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

partially lethal, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

partially lethal - majority die, with Scer\GAL4^elav-C155

(Xiao et al., 2019)

partially lethal - majority die, with Scer\GAL4^NP3084

(Xiao et al., 2019, Xiao et al., 2014)

partially lethal - majority die | nutrition conditional, with Scer\GAL4^NP3084

(Xiao et al., 2019)

some die during larval stage, with Scer\GAL4^NP3084

(Xiao et al., 2019)

some die during pupal stage, with Scer\GAL4^NP3084

(Xiao et al., 2019)

some die during pupal stage, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^elav-C155

(Xiao et al., 2019)

visible | adult stage, with Scer\GAL4^GMR.PFa

(Xiao et al., 2019)

Phenotype Manifest In

eye, with Scer\GAL4^elav-C155

(Xiao et al., 2019)

eye, with Scer\GAL4^GMR.PFa

(Xiao et al., 2019)

female germline stem cell, with Scer\GAL4^Lsp2.3.1

(Matsuoka et al., 2017)

Detailed Description

Statement

Reference

Expressing Fer1HCH^GD4853 under the control of Scer\GAL4^NP3084 is lethal in flies fed an iron-depleted diet and only partially lethal (majority die) when flies are fed a regular or iron-rich diet. Expression under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^GMR.PFa results in slightly smaller eyes with a rough appearance.

(Xiao et al., 2019)

Expression of Fer1HCH^GD4853 under the control of Scer\GAL4^Lsp2.3.1 (using tub-Gal80[ts] to limit expression to adulthood) does not induce cell death in either germaria or vitellogenic stage follicles and while it does not affect the proliferation of the germline stem cells it leads to significant increase in the rate of their loss over time.

(Matsuoka et al., 2017)

Scer\GAL4^NP3084-mediated expression of Fer1HCH^GD4853 decreases total body iron content and causes death at larval/pupal stages.

(Xiao et al., 2014)

Expression with P{GAL4-tim.E}27, in the presence of Dcr-2^Scer\UAS.cDa, results in lethality.

(Mandilaras and Missirlis, 2012)

Adults expressing Fer1HCH^GD4853 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4853

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Fer1HCH^GD4853, Scer\GAL4^NP3084 has increased mortality during development phenotype, enhanceable by Tsf1^UAS.cXa, Scer\GAL4^NP3084

(Xiao et al., 2019)

NOT Enhanced by

Statement

Reference

Fer1HCH^GD4853, Scer\GAL4^NP3084 has lethal | nutrition conditional phenotype, non-enhanceable by Tsf1^UAS.cXa, Scer\GAL4^NP3084

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^NP3084 has partially lethal - majority die | nutrition conditional phenotype, non-enhanceable by Tsf1^UAS.cXa, Scer\GAL4^NP3084

(Xiao et al., 2019)

Suppressed by

Statement

Reference

Fer1HCH^GD4853, Scer\GAL4^NP3084 has increased mortality during development phenotype, suppressible | partially by Tsf1^KK103815, Scer\GAL4^NP3084

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^NP3084 has lethal | nutrition conditional phenotype, suppressible | partially by Tsf1^KK103815, Scer\GAL4^NP3084

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^da.G32 has lethal - all die during second instar larval stage phenotype, suppressible | partially by Tsf1^KK103815, Scer\GAL4^da.G32

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^Cg.PA has lethal - all die during second instar larval stage phenotype, suppressible | partially by Scer\GAL4^da.G32/Tsf1^KK103815

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^NP3084 has partially lethal - majority die phenotype, suppressible | partially by Zip99C^UAS.cXa, Scer\GAL4^NP3084

(Xiao et al., 2014)

NOT suppressed by

Statement

Reference

Fer1HCH^GD4853, Scer\GAL4^NP3084 has lethal | nutrition conditional phenotype, non-suppressible by Tsf1^UAS.cXa, Scer\GAL4^NP3084

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^NP3084 has partially lethal - majority die | nutrition conditional phenotype, non-suppressible by Tsf1^UAS.cXa, Scer\GAL4^NP3084

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^elav-C155 has partially lethal - majority die phenotype, non-suppressible by Tsf1^KK103815, Scer\GAL4^elav-C155

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^elav-C155 has visible | adult stage phenotype, non-suppressible by Tsf1^KK103815, Scer\GAL4^elav-C155

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^elav-C155 has abnormal size | adult stage phenotype, non-suppressible by Tsf1^KK103815, Scer\GAL4^elav-C155

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^GMR.PFa has visible | adult stage phenotype, non-suppressible by Tsf1^KK103815, Scer\GAL4^GMR.PFa

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^GMR.PFa has abnormal size | adult stage phenotype, non-suppressible by Tsf1^KK103815, Scer\GAL4^GMR.PFa

(Xiao et al., 2019)

Enhancer of

Statement

Reference

Fer1HCH^GD4853, Scer\GAL4^da.G32 is an enhancer of partially lethal - majority live phenotype of Scer\GAL4^da.G32, Tsf1^KK103815

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^Cg.PA is an enhancer of partially lethal - majority live phenotype of Scer\GAL4^Cg.PA, Tsf1^KK103815

(Xiao et al., 2019)

Other

Statement

Reference

Fer1HCH^GD4853, Scer\GAL4^NP3084, Tsf1^KK103815 has partially lethal - majority die phenotype

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^NP3084, Tsf1^UAS.cXa has lethal phenotype

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^da.G32, Tsf1^KK103815 has partially lethal - majority die phenotype

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^Cg.PA, Scer\GAL4^da.G32, Tsf1^KK103815 has partially lethal - majority die phenotype

(Xiao et al., 2019)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Fer1HCH^GD4853, Scer\GAL4^elav-C155 has eye phenotype, non-suppressible by Tsf1^KK103815, Scer\GAL4^elav-C155

(Xiao et al., 2019)

Fer1HCH^GD4853, Scer\GAL4^GMR.PFa has eye phenotype, non-suppressible by Tsf1^KK103815, Scer\GAL4^GMR.PFa

(Xiao et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing Tsf1^KK103815 with Fer1HCH^GD4853 under the control of Scer\GAL4^NP3084 results in partial lethality (majority die) regardless of iron content in the diet.

(Xiao et al., 2019)

The low eclosion rate and decreased total body iron content of Scer\GAL4^NP3084 Fer1HCH^GD4853 flies can be partially rescued by co-expression of Zip99C^Scer\UAS.cXa.

(Xiao et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4853

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Fer1HCH^GD4853

(Matsuoka et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (16)

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