UASt regulatory sequences drive expression of an inverted repeat.
Expressing StripGD7322 under the control of either Scer\GAL4C587 or Scer\GAL4tj.PU (together with Dicer-2, for a more efficient RNAi) leads to L3 male gonad defects: small gonads with supernumerary strongly DAPI-positive cells throughout, whereas control gonads exhibit brighter DAPI-stained DNA in the apical region of the tissue, where the GSCs, gonialblasts, TA spermatogonia, CySCs and CyCs reside. Expression under the control of Scer\GAL4upd1.PU or Scer\GAL4nos.PU does not yield any obvious phenotype.
Adults expressing StripGD7322 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in early pupal lethality or late pupal lethality.
Scer\GAL4Mef2.PR, StripGD7322 has increased body size | P-stage phenotype, enhanceable by NuakK99R
Scer\GAL4Mef2.PR, StripGD7322 has increased body size | P-stage phenotype, enhanceable by stv1
StripGD7322, Scer\GAL4Myo31DF.PU is a suppressor of increased occurrence of cell division | adult stage phenotype of PXoHMS01023, Scer\GAL4Myo31DF.PU
StripGD7322, Scer\GAL4esg.PU is a suppressor of increased occurrence of cell division | adult stage phenotype of PXoHMS01023, Scer\GAL4esg.PU
Scer\GAL4Mef2.PR, StripGD7322 has embryonic/larval hypodermal muscle | larval stage phenotype, enhanceable by NuakK99R
Scer\GAL4Mef2.PR, StripGD7322 has embryonic/larval hypodermal muscle | larval stage phenotype, enhanceable by stv1
StripGD7322, Scer\GAL4Myo31DF.PU is a suppressor of adult midgut phenotype of PXoHMS01023, Scer\GAL4Myo31DF.PU
StripGD7322, Scer\GAL4esg.PU is a suppressor of adult midgut phenotype of PXoHMS01023, Scer\GAL4esg.PU