Third instar larvae expressing Tip60^{dsRNA.Z.Scer\UAS} under the control of Scer\GAL4^elav-C155 are significantly less mobile and perform significantly fewer full body peristaltic contractions in a locomotor assay compared to controls. The time taken for the larvae to right themselves after being turned ventral side up is also significantly longer than normal.

Expression of Tip60^{dsRNA.Z.Scer\UAS} under the control of Scer\GAL4^P2.4.Pdf has no adverse effect on the axonal pattern development in third instar larvae but leads to loss of s-LNv terminal synaptic arborization and reduced axon length in the adult brain compared to controls. It also causes nighttime sleep disruption and fragmentation as well as daytime sleepiness.

Pre-synaptic expression of Tip60^{dsRNA.Z.Scer\UAS} under the control of Scer\GAL4^elav-C155 results in pupal lethality.There is a significant increase in the total number of synaptic boutons in Tip60^{E431Q.Scer\UAS}-expressing larvae, when compared to wild-type controls. There is a substantially larger expansion of type-I boutons compared to type-Ib in these mutants. There is also a slight increase in the number of satellite boutons in these mutants compared to controls. This increase in bouton number is accompanied by an excess of futsch-associated microtubule looping and splaying.

Post-synaptic expression of Tip60^{dsRNA.Z.Scer\UAS} under the control of Scer\GAL4^Mef2.247 results in pupal lethality. Mutant larvae exhibit a significant reduction in bouton number, specifically in 1s boutons, as well as the absence of satellite boutons.

Expression of Tip60^{dsRNA.Z.Scer\UAS} under the control of Scer\GAL4^how-24B results in a reduction in viability. The flies die at a broad range of developmental stages, depending on the insertion line used, beginning from early pupae to directly before eclosion. The apparent cause of lethality later in development appears to be due to an inability to eclose from the pupal casing.