FB2024_03 , released June 25, 2024
Allele: Dmel\Pvr1
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General Information
Symbol
Dmel\Pvr1
Species
D. melanogaster
Name
FlyBase ID
FBal0197800
Feature type
allele
Associated gene
Dmel\Pvr
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Bruckner et al., 2004)
Mutagen
ethyl methanesulfonate
(Bruckner et al., 2004)
Progenitor genotype
Cytology
Description

Amino acid replacement: W1087term.

(Bruckner et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:8,223,121..8,223,121 [-]
Nucleotide change:

G8223121A

Amino acid change:

W1087term | Pvr-PA; W1087term | Pvr-PB; W1087term | Pvr-PD; W1081term | Pvr-PE; W1046term | Pvr-PG; W1052term | Pvr-PH; W1155term | Pvr-PI; W1155term | Pvr-PJ; W1078term | Pvr-PK; W1087term | Pvr-PL; W1146term | Pvr-PM; W1077term | Pvr-PN; W1077term | Pvr-PO

Reported amino acid change:

W1087term

Comment:

Reported as a change in Trp 1087 from TGG to TGA.

(Bruckner et al., 2004)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Pvr1 mutants exhibit a severe hemocyte migration defect in which the cells are unable to migrate from their origin in the head and undergo apoptosis.

      (Wood et al., 2006)

      By stage 12, Pvr1 homozygous embryos contain large clusters of macrophages.

      Macrophages in stage 16 Pvr1 homozygous embryos contain multiple engulfed apoptotic hemocytes.

      (Bruckner et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Pvr1 has increased cell death | recessive phenotype, suppressible by Scer\GAL4srp.Hemo/BacA\p35UAS.cHa

      (Bruckner et al., 2004)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Border cells which are part of somatic clones of Pvr1; Egfrf2 double homozygous cells initiate migration, but fail to reach the oocyte.

      (Jekely et al., 2005)

      Ras85DV12.Scer\UAS; Scer\GAL4srp.Hemo suppresses the macrophage aggregation phenotype of stage 16 Pvr1 homozygous embryos. However, these embryos have a mild deficiency of hemocytes in the ventral-posterior region and in the posterior end of the elongated germ band.

      Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX; Scer\GAL4srp.Hemo suppresses the reduction in hemocytes seen at stage 12 in Pvr1 homozygous embryos, but fails to suppress the reduction in hemocyte numbers at stage 16 and only mildly suppresses the macrophage aggregation phenotype.

      (Bruckner et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference

      The Pvr1 hemocyte migration phenotype can be suppressed through expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4srp.Hemo. The rescued hemocytes are not only unable to inflitrate the germ band, but also fail to migrate posteriorly from the head along the central nervous system and the dorsal vessel.

      Pvr1 BacA\p35Scer\UAS.cHa (Scer\GAL4Pxn.PS) mutants show a robust wild-type response to laser ablation, indicating that hemocyte chemotaxis towards wounds is Pvr independent.

      (Wood et al., 2006)

      Clustering of macrophages, the presence of engulfed apoptotic hemocytes in macrophages and reduction in hemocyte numbers in Pvr1 embryos are all eliminated by BacA\p35Scer\UAS.cHa; Scer\GAL4srp.Hemo. However, hemocyte distribution is still abnormal in these embryos.

      (Bruckner et al., 2004)
      Complementation and Rescue Data
      Rescued by

      Pvr1 is rescued by Pvrtub.PB

      (Bruckner et al., 2004)

      Pvr1 is rescued by Pvrtub.PB

      (Bruckner et al., 2004)
      Partially rescued by

      Pvr1 is partially rescued by Pvrλ.UASp.Tag:MYC/Scer\GAL4srp.Hemo

      (Bruckner et al., 2004)
      Comments

      Pvrtub.PB completely rescues the macrophage aggregation and distribution phenotypes of stage 16 Pvr1 homozygous embryos.

      Pvrλ.Scer\UAS.T:Hsap\MYC; Scer\GAL4srp.Hemo rescues the macrophage aggregation phenotype of stage 16 Pvr1 homozygous embryos. However, these embryos retain a mild deficiency of hemocytes in the ventral-posterior region and in the posterior end of the elongated germ band.

      (Bruckner et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Isolated in an EMS screen using FRT40/FRT40 males, recovering single chromosomes and testing for lethality and sterility over Df(2L)TE29Aa-1.

      (Bruckner et al., 2004)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pvr1
      (Poukkula et al., 2011, Janssens et al., 2010, Bruckner et al., 2004, Bruckner et al., 2004)
      pvr1
      (Siekhaus et al., 2010, Wood et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)