Insertion within an intron located between two coding exons, in the appropriate predicted reading frame to fuse the GFP exon in the inserted construct to the endogenous protein.
P{PTT-un1} insertion within first intron of the Rm62 gene.
nurse cell & nuclear chromosome (with Rm6201086)
Rm62CB02119/Rm6201086 mutant flies infected with the bunyavirus Rift Valley Fever Virus (RVFV) exhibit a dramatic increase in mortality compared to sibling controls. The survival of uninfected flies is unaffected. Increased mortality is also seen when Rm62CB02119/Rm6201086 mutant flies are infected with another bunyavirus, La Crosse Virus (LACV). No increase in mortality is seen with Drosophila C virus (DCV), Vesicular stomatitis virus (VSV) or Sindbis virus (SINV) infection.
Rm62CB02119/Rm6201086 adults have reduced, thin, or missing dorsocentral and scutellar bristles. Rm62CB02119/Rm6201086 females are sterile; the nurse cell chromosomes of the follicles of these female mutants fails to disperse during the fifth endocycle and these follicles have degenerated by stage 8.
Rm62CB02119/Rm62F mutants cells show defective RNA export as labelled RNA remains associated with the chromosomes in a tight banded pattern instead of diffusing throughout the nucleoplasm and cytoplasm, as occurs in control cells. Furthermore, the export factors sbr and Aly are redistributed within the nuclei of the mutant cells. Rm62CB02119/Rm62F cells also show defects in gene deactivation as evidenced by Br-UTP incorporation experiments.
Some Rm62CB02119/Rm62F transheterozygous mutants remain as third instar larvae for up to 5 days, whereas control animals enter prepupal development after 12 days.
The Rm62CB02119 allele expresses a small amount of normal Rm62.
Protein trap line.