egg chamber & mitochondrion | germ-line clone
germline cyst & mitochondrion | germ-line clone
oocyte & mitochondrion | germ-line clone
Mitochondria aggregate prematurely near and within the oocyte in prefollicular cysts from miltEY01559 germ-line clones. The mitochondria produce a giant Balbiani body that fills the ooplasm of the oocyte at follicle formation. Mitochondrial number remains elevated at stage 5, but normalizes by stage 9. However, these mitochondria concentrate abnormally at microtubule minus ends in the anterior corners of the follicles.
The fusomes of miltEY01559 cysts are indistinguishable from wild type throughout oogenesis and organelles that associate with the Balbiani body, such as Golgi elements, concentrate and move into the oocyte normally.
miltEY01559 females lay eggs with normal mitochondrial DNA content which can form pole cells and that can be fertilized to give rise to viable, fertile adults.