Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w+mC and the mini-yellow marker y+mDint2, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
The EY01559 line may have originally contained two insertions, one on 2L and one on 3L. The stock currently in the Bloomington Drosophila Stock Center only contains the 2L insertion, in the milt gene.