FB2024_03 , released June 25, 2024
Allele: Dmel\DarkUAS.Tag:polyHis,Tag:MYC
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General Information
Symbol
Dmel\DarkUAS.Tag:polyHis,Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0193514
Feature type
allele
Associated gene
Dmel\Dark
Associated Insertion(s)
Carried in Construct
P{UAS-Dark.WT}
Also Known As
UAS-darkWT, uas-Dark, UAS-ark
Key Links
Transgenic product class
wild_type
(Akdemir et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Akdemir et al., 2006)
Mutagen
in vitro construct
(Akdemir et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Dark.WT}
Cytology
Description

UASt regulatory sequences drive expression of Dark coding sequences with 8xTag:polyHis at the N-terminal end and 6xTag:MYC tags at the carboxy terminal end.

(Akdemir et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Dark
Tagged with
Tag:MYC
Tag:polyHis
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC in the developing eye-antennal discs, under the control of Scer\GAL4GMR.PF does not induce significant apoptosis.

      (Verghese et al., 2012)

      Expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC in hemocytes, under the control of Scer\GAL4Hml.PG only mildly restores apoptosis after Smac mimetic treatment.

      (Akdemir et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      NOT suppressed by
      Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Embryos co-expressing Droncpro.Scer\UAS and DarkScer\UAS.T:Zzzz\His6,T:Hsap\MYC in neurons under the control of Scer\GAL4elav.PU exhibit increased engulfment of apoptotic neurons.

      (Shklyar et al., 2013)

      The combined expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF generates a potent eye ablation phenotype.

      Either a BruceE81/+ or BruceE23/+ heterozygous background has no effect on the eye ablation phenotype found upon co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF.

      (Domingues and Ryoo, 2012)

      Co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS together, under the control of Scer\GAL4GMR.PF induces massive apoptosis in the eye-antennal discs and adult eyes.

      Co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC, Ncpro.Scer\UAS and hpoScer\UAS.cUa in the developing eye, under the control of Scer\GAL4GMR.PF induces massive apoptosis in the eye-antennal disc and adult. This phenotype appears stronger than controls as both photoreceptor and pigment cells are eventually eliminated in animals co-expressing hpoScer\UAS.cUa.

      Co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS with ykiScer\UAS.cHa, all under the control of Scer\GAL4GMR.PF, completely suppresses the overgrowth phenotype of ykiScer\UAS.cHa overexpression, due to induction of apoptosis in response to increased Nc activity.

      (Verghese et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC
      DarkScer\UAS.T:Zzzz\His6,T:Hsap\MYC
      DarkUAS.Tag:polyHis,Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (8)