FlyBase Allele Report: Dmel\Bruce[E81]

General Information

Symbol

Dmel\Bruce^E81

Species

D. melanogaster

Name

FlyBase ID

FBal0162760

Feature type

allele

Associated gene

Dmel\Bruce

Associated Insertion(s)

Carried in Construct

Also Known As

dBruce^E81

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Bruce^E81 contains an in frame deletion that removes the BIR domain.

(Domingues and Ryoo, 2012)

Deletion of 534 bp in-frame = codons 234-411 (predicted to encode the BIR domain).

(Arama et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Nezis et al., 2010)

increased cell death | adult stage

(Hou et al., 2008)

Phenotype Manifest In

egg chamber

(Nezis et al., 2010, Hou et al., 2008)

female germline cyst

(Hou et al., 2008)

germarium region 2

(Hou et al., 2008)

Detailed Description

Statement

Reference

Mutant females have a significant increase in the number of degenerating egg chambers during mid-oogenesis compared to wild type.

(Nezis et al., 2010)

Well-fed Bruce^E81 mutant flies display increased levels of autophagy (punctate Lysotracker staining) in region two of the germarium and in stage eight degenerating egg chambers compared to controls.

In contrast to wild type, the ovaries of well-fed Bruce^E81 mutant flies contain a large number of degenerating stage eight egg chambers.

An increased number of apoptotic (TUNEL positive) cells are seen in region two cysts in well-fed Bruce^E81 mutant flies than are seen in wild type. TUNEL positive cells are also seen in Bruce^E81 mutant stage eight egg chambers.

(Hou et al., 2008)

Bruce^E81 homozygotes are viable but male-sterile. Mutant spermatid nuclei are significantly more condensed and rounded than wild-type, and are scattered throughout the cyst. In addition, there are fewer nuclei, and many nuclei are stained faintly, presumably because they undergo degeneration. Although an indivdualisation complex forms, it is highly reduced and scattered.

(Arama et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Bruce^E81/Bruce[+] is an enhancer of eye phenotype of rpr^GMR.PD

(Domingues and Ryoo, 2012)

NOT Enhancer of

Statement

Reference

Bruce^E81/Bruce[+] is a non-enhancer of pigment cell phenotype of Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Domingues and Ryoo, 2012)

Bruce^E81/Bruce[+] is a non-enhancer of eye phenotype of Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Domingues and Ryoo, 2012)

Bruce^E81/Bruce[+] is a non-enhancer of pigment cell phenotype of Dark^{UAS.Tag:polyHis,Tag:MYC}, Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Domingues and Ryoo, 2012)

Bruce^E81/Bruce[+] is a non-enhancer of eye phenotype of Dark^{UAS.Tag:polyHis,Tag:MYC}, Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Domingues and Ryoo, 2012)

NOT Suppressor of

Statement

Reference

Bruce^E81/Bruce[+] is a non-suppressor of pigment cell phenotype of Dark^{UAS.Tag:polyHis,Tag:MYC}, Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Domingues and Ryoo, 2012)

Bruce^E81/Bruce[+] is a non-suppressor of eye phenotype of Dark^{UAS.Tag:polyHis,Tag:MYC}, Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Domingues and Ryoo, 2012)

Bruce^E81/Bruce[+] is a non-suppressor of pigment cell phenotype of Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Domingues and Ryoo, 2012)

Bruce^E81/Bruce[+] is a non-suppressor of eye phenotype of Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Domingues and Ryoo, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

A Bruce^E81 heterozygous background dominantly enhances the rpr^GMR.PD eye ablation phenotype.

A Bruce^E81 heterozygous background does not affect the pigment cell death phenotype caused by the overexpression of Nc^pro.Scer\UAS in the developing eye under the control of Scer\GAL4^GMR.PF.

A Bruce^E81/+ heterozygous background has no effect on the eye ablation phenotype found upon co-expression of Ark^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} and Nc^pro.Scer\UAS in the developing eye under the control of Scer\GAL4^GMR.PF.

(Domingues and Ryoo, 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Bruce^E81

(Hou et al., 2008)

dBruce^E81

(Domingues and Ryoo, 2012, Nezis et al., 2010)

dbruce^E81

(Rathke et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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