A heat shock promoter drives expression of a mutated Sb cDNA.
Amino acid replacement: S737A.
Expression of SbS737A.hs using heat shock at 0 or 3 hours after pupariation (AP), or a double heat shock at both 0 and 3 hours AP has no effect on leg morphogenesis.
97% of flies expressing two copies of SbS737A.hs (using four 1 hour heat shocks at 25, 27, 29 and 31 hours AP) in a Df(3R)sbd105/+ background have a short-bristle phenotype.
SbS737A.hs fails to rescue Sbsbd-2
SbS737A.hs fails to rescue Df(3R)sbd105/Sbsbd-201