FlyBase Allele Report: Dmel\Gyc89Db[MB03197]

General Information

Symbol

Dmel\Gyc89Db^MB03197

Species

D. melanogaster

Name

FlyBase ID

FBal0192414

Feature type

allele

Associated gene

Dmel\Gyc89Db

Associated Insertion(s)

Mi{ET1}Gyc89Db^MB03197

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{ET1}Gyc89Db^MB03197

Cytology

Description

Insertion within the coding region, 1510bp downstream of the translational start site.

(Vermehren-Schmaedick et al., 2010)

Allele components

Component

Use(s)

Inserted element

Mi{ET1}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:16,480,337..16,480,337 [-]

(Vermehren-Schmaedick et al., 2011, Vermehren-Schmaedick et al., 2010, Bellen, 2007.4.5)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response | third instar larval stage

(Vermehren-Schmaedick et al., 2010)

abnormal taste perception

(Vermehren-Schmaedick et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Gyc-89Db^MB03197 mutant larvae show a similar avoidance to caffeine as wild-type larvae, but do not show a preference for sucrose, as wild-type larvae do.

Gyc-89Db^MB03197 mutants show a similar attraction to 1% agar containing 100mM sucrose as wild-type. They show similar avoidance to 1% agar with 10mm caffeine as in wild-type but fail to show caffeine avoidance to lower levels of caffeine such as 1mM caffeine in 1% agar.

Recordings from s6, a S-type sensilla of the labellum, reveal a significantly reduced response to 2.5mM caffeine in Gyc-89Db^MB03197 mutants. The mean number of impulses recorded is about half that from control flies.

(Vermehren-Schmaedick et al., 2011)

Homozygous third instar larvae do not show defects in general locomotion.

Homozygous third instar larvae show a defect in the normal hypoxia escape response; mutant larvae which have been allowed to burrow into the food and commence feeding take significantly longer than control larvae to leave the food when exposed to low oxygen concentrations.

Mutant larvae shifted to a severe hypoxic environment (11-15% oxygen) respond in the same manner as wild-type controls, with a significant reduction in the number of stops and turns in their locomotion. However, if they are shifted to mildly hypoxic (17-20% oxygen) or hyperoxic (22-30% oxygen) conditions the mutant larvae show no behavioural response and maintain their area-restricted search with a high number of stops and turns (wild-type controls show a significant reduction in the number of stops and turns under these conditions).

(Vermehren-Schmaedick et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gyc89Db^MB03197 has abnormal taste perception phenotype, enhanceable by Gyc89Da^e01821

(Vermehren-Schmaedick et al., 2011)

Suppressed by

Statement

Reference

Gyc89Db^MB03197 has abnormal taste perception phenotype, suppressible by Scer\GAL4^Gyc89Db.3.4/Gyc89Da^UAS.cVa

(Vermehren-Schmaedick et al., 2011)

Gyc89Db^MB03197 has abnormal stress response | third instar larval stage phenotype, suppressible by Gyc89Da^UAS.cVa/Scer\GAL4^Gyc89Da.1.9

(Vermehren-Schmaedick et al., 2010)

Gyc89Db^MB03197 has abnormal stress response | third instar larval stage phenotype, suppressible by Scer\GAL4^Gyc89Db.3.4/Gyc89Da^UAS.cVa

(Vermehren-Schmaedick et al., 2010)

NOT suppressed by

Statement

Reference

Gyc89Db^MB03197 has abnormal taste perception phenotype, non-suppressible by Gyc89Da^UAS.cVa/Scer\GAL4^Gyc89Da.1.9

(Vermehren-Schmaedick et al., 2011)

Enhancer of

Statement

Reference

Gyc89Db^MB03197 is an enhancer of abnormal taste perception phenotype of Gyc89Da^e01821

(Vermehren-Schmaedick et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Larvae double mutant for Gyc-89Da^e01821 and Gyc-89Db^MB03197 show no attraction to sucrose and fail to avoid caffeine.

The preference for sucrose in Gyc-89Db^MB03197 or Gyc-89Db^MB03197/Gyc-89Da^e01821 double mutants can be rescued through expression of Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Db.3.4. In contrast, expression of Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Da.1.9 fails to suppress the sucrose ambivalence phenotype.

Gyc-89Da^e01821/Gyc-89Db^MB03197 double mutants exhibit a significantly reduced response to 2.5mM caffeine, more so than in either single mutant.

(Vermehren-Schmaedick et al., 2011)

The defect in the hypoxia escape response that is seen in Gyc-89Db^MB03197 larvae is rescued by expression of Gyc-89Da^Scer\UAS.cVa under the control of either Scer\GAL4^Gyc-89Da.1.9 or Scer\GAL4^Gyc-89Db.3.4.

The failure to change larval exploratory behaviour that is seen in Gyc-89Db^MB03197 larvae downshifted from 21 to 19% oxygen or upshifted from 21 to 25% oxygen is rescued by expression of Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Db.3.4, but is not rescued by expression of Gyc-89Da^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Da.1.9.

(Vermehren-Schmaedick et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Gyc89Db^MB03197 is rescued by Gyc89Db^UAS.cVa/Scer\GAL4^Gyc89Da.1.9

(Vermehren-Schmaedick et al., 2010)

Gyc89Db^MB03197 is rescued by Gyc89Db^UAS.cVa/Scer\GAL4^Gyc89Db.3.4

(Vermehren-Schmaedick et al., 2010)

Comments

The preference for sucrose in Gyc-89Db^MB03197 or Gyc-89Db^MB03197/Gyc-89Da^e01821 double mutants can be rescued through expression of Gyc-89Db^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Db.3.4. In contrast, expression of Gyc-89Db^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Da.1.9 fails to suppress the sucrose ambivalence phenotype.

The loss of caffeine avoidance behaviour at low concentrations of caffeine in Gyc-89Db^MB03197 mutants is rescued by expressing Gyc-89Db^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Da.1.9.

(Vermehren-Schmaedick et al., 2011)

The defect in the hypoxia escape response that is seen in Gyc-89Db^MB03197 larvae is rescued by expression of Gyc-89Db^Scer\UAS.cVa under the control of either Scer\GAL4^Gyc-89Da.1.9 or Scer\GAL4^Gyc-89Db.3.4.

The failure to change larval exploratory behaviour that is seen in Gyc-89Db^MB03197 larvae downshifted from 21 to 19% oxygen or upshifted from 21 to 25% oxygen is rescued by expression of Gyc-89Db^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Db.3.4, but is not rescued by expression of Gyc-89Db^Scer\UAS.cVa under the control of Scer\GAL4^Gyc-89Da.1.9.

(Vermehren-Schmaedick et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

23519

w¹¹¹⁸; Mi{ET1}Gyc89Db^MB03197

93108

w^*; PBac{RB}Gyc89Da^e01821 Mi{ET1}Gyc89Db^MB03197

Notes on Origin

Discoverer