FlyBase Allele Report: Dmel\Ctr1B[UAS.cSa]

General Information

Symbol

Dmel\Ctr1B^UAS.cSa

Species

D. melanogaster

Name

UAS construct a of Selvaraj

FlyBase ID

FBal0191931

Feature type

allele

Associated gene

Dmel\Ctr1B

Associated Insertion(s)

Carried in Construct

P{UAS-Ctr1B.S}

Also Known As

UAS-Ctr1B

Key Links

Transgenic product class

cDNA

(Selvaraj et al., 2005)

wild_type

(Selvaraj et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Selvaraj et al., 2005)

wild_type

(Selvaraj et al., 2005)

Mutagen

in vitro construct

(Selvaraj et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Ctr1B.S}

Cytology

Description

UAS regulatory sequences drive expression of Ctr1B cDNA sequences.

(Selvaraj et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Ctr1B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Act5C.PU

(Steiger et al., 2010, Selvaraj et al., 2005)

lethal, with Scer\GAL4^GMR.PF

(Hua et al., 2010)

partially lethal - majority die | larval stage, with Scer\GAL4^Act5C.PI

(Hua et al., 2010)

partially lethal - majority die | pupal stage, with Scer\GAL4^Act5C.PI

(Hua et al., 2010)

visible | conditional, with Scer\GAL4^GMR.PF

(Egli et al., 2006)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Hua et al., 2010, Steiger et al., 2010)

eye | conditional, with Scer\GAL4^GMR.PF

(Egli et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Steiger et al., 2010)

Detailed Description

Statement

Reference

Ubiquitous and constitutive overexpression of Ctr1B^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PI leads to complete larval and pupal lethality when the flies are raised on normal food. The observed lethality is copper-dependent, since a large fraction of the flies successfully reach the adult stage if they are raised on food containing the copper-specific metal chelator BCS.

Overexpression of Ctr1B^Scer\UAS.cSa in the eye under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype in flies raised on normal food. This phenotype is strongly aggravated in flies raised on 100 υM copper. The strong rough eye phenotype is accompanied by high lethality. A 250υM BCS (a copper chelator) food supplement completely restores the wild-type eye phenotype and wild-type levels of survival.

(Hua et al., 2010)

Strong, ubiquitous expression of Ctr1B^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PU is lethal when the flies are raised on normal food. Such flies can be partially rescued (<50%) by growing them on food supplemented with the copper chelator BCS.

Strong, eye-specific expression of Ctr1B^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF leads to severely disrupted eye morphology (the rigid quasi-crystalline pattern of the ommatidia is lost and ommatidia are fused, leading to an uneven eye surface; in addition, overall eye shape is changed) when raised on normal food. This rough eye phenotype can be fully rescued by the addition of the copper chelator BCS.

(Steiger et al., 2010)

Expression of Ctr1B^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype when the flies are raised on food supplemented with 20μM copper.

(Egli et al., 2006)

Flies with the Ctr1B^Scer\UAS.cSa transgene, under the regulation of Scer\GAL4^Act5C are lethal. In the presence of bathocuproinedisulfonate (a copper chelator), these flies survive.

(Selvaraj et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF has visible | conditional phenotype, suppressible by MTF-1^UAS.cEa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF has visible | conditional phenotype, suppressible by MtnA^UAS.cEa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF has visible | conditional phenotype, suppressible by MtnB^UAS.cEa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

NOT suppressed by

Statement

Reference

Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF has visible | conditional phenotype, non-suppressible by MtnC^UAS.cEa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF has visible | conditional phenotype, non-suppressible by MtnD^UAS.cEa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

Suppressor of

Statement

Reference

Scer\GAL4^Act5C.PU/Ctr1B^UAS.cSa is a suppressor of lethal | third instar larval stage phenotype of MTF-1^140-1R

(Selvaraj et al., 2005)

Scer\GAL4^Act5C.PU/Ctr1B^UAS.cSa is a suppressor of abnormal developmental rate phenotype of MTF-1^140-1R

(Selvaraj et al., 2005)

NOT Suppressor of

Statement

Reference

Ctr1B^UAS.cSa/Scer\GAL4^Tub.PU is a non-suppressor of lethal | larval stage phenotype of Ctr1A²⁵

(Hua et al., 2010)

Ctr1B^UAS.cSa/Scer\GAL4^Tub.PU is a non-suppressor of abnormal developmental rate | larval stage phenotype of Ctr1A²⁵

(Hua et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF has eye | conditional phenotype, suppressible by MtnA^UAS.cEa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF has eye | conditional phenotype, suppressible by MtnB^UAS.cEa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF has eye | conditional phenotype, suppressible by MTF-1^UAS.cEa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

NOT suppressed by

Statement

Reference

Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF has eye | conditional phenotype, non-suppressible by MtnC^UAS.cEa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF has eye | conditional phenotype, non-suppressible by MtnD^UAS.cEa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of Ctr1B^Scer\UAS.cSa under the control of Scer\GAL4^tub fails to suppress the lethality associated with developmental arrest found in Ctr1A²⁵ mutants. This failure could be associated with toxicity caused by excessive copper uptake.

(Hua et al., 2010)

The rough eye phenotype caused by expression of Ctr1B^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF in the presence of 20μM copper is suppressed by co-expression of one of MTF-1^Scer\UAS.cEa, MtnA^Scer\UAS.cEa or MtnB^Scer\UAS.cEa, but is not suppressed by co-expression of one of MtnC^Scer\UAS.cEa or MtnD^Scer\UAS.cEa.

(Egli et al., 2006)

Constitutive expression of Ctr1B^Scer\UAS.cSa, under the regulation of Scer\GAL4^Act5C rescues the developmental arrest and larval lethality of MTF-1^140-1R mutants under copper depletion, with several viable MTF-1^140-1R Ctr1B^Scer\UAS.cSa (Scer\GAL4^Act5C) flies surviving on food containing 50 or even 100υM bathocuproinedisulfonate (a copper chelator). The rescued flies are normal and fertile.

(Selvaraj et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ctr1B^Scer\UAS.cSa

Ctr1B^UAS.cSa

Name Synonyms

UAS construct a of Selvaraj

(Selvaraj et al., 2005)

Secondary FlyBase IDs

References (4)

Report Sections

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